A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic Rhinosinusitis
Objectives This case-controlled study aimed to identify the association of tumor necrosis factor (TNF)α-1031 and TNFβ+ 252 gene polymorphisms between chronic rhinosinusitis (CRS) and healthy controls. Another purpose of this study was to investigate the associations of these gene polymorphisms with...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Korean Society of Otorhinolaryngology-Head and Neck Surgery
2017-09-01
|
Series: | Clinical and Experimental Otorhinolaryngology |
Subjects: | |
Online Access: | http://www.e-ceo.org/upload/pdf/ceo-2016-01732.pdf |
id |
doaj-232eb6634c5c467d83c6f0a1183a5ec1 |
---|---|
record_format |
Article |
spelling |
doaj-232eb6634c5c467d83c6f0a1183a5ec12020-11-24T23:59:53ZengKorean Society of Otorhinolaryngology-Head and Neck SurgeryClinical and Experimental Otorhinolaryngology1976-87102005-07202017-09-0110324124710.21053/ceo.2016.01732533A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic RhinosinusitisKhairunnisak Misron0Suzina Sheikh Ab Hamid1Azlina Ahmad2Ramiza Ramza Ramli3 Department of Otorhinolaryngology-Head & Neck Surgery, School of Medical Sciences, Universiti Sains Malaysia Health Campus, Kelantan, Malaysia Department of Otorhinolaryngology-Head & Neck Surgery, School of Medical Sciences, Universiti Sains Malaysia Health Campus, Kelantan, Malaysia Basic Science and Oral Biology Unit, School of Dental Sciences, Universiti Sains Malaysia Health Campus, Kelantan, Malaysia Department of Otorhinolaryngology-Head & Neck Surgery, School of Medical Sciences, Universiti Sains Malaysia Health Campus, Kelantan, MalaysiaObjectives This case-controlled study aimed to identify the association of tumor necrosis factor (TNF)α-1031 and TNFβ+ 252 gene polymorphisms between chronic rhinosinusitis (CRS) and healthy controls. Another purpose of this study was to investigate the associations of these gene polymorphisms with factors related to CRS. Methods All deoxyribonucleic acid (DNA) samples were genotyped for TNFα-1031 and TNFβ+252 genes by mean of polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP). The statistical analysis were carried out using chi-square test or Fisher exact test to determine the associations of these gene polymorphisms in CRS. Multiple logistic regression was performed to evaluate the associations of these gene polymorphisms in CRS and its related risk factors. Results The genotype and allele frequencies of TNFα-1031 and TNFβ+252 gene did not show any significant associations between CRS and healthy controls. However, a significantly statistical difference of TNFα-1031 was observed in CRS participants with atopy (P-value, 0.045; odds ratio, 3.66) but not in CRS with asthma or aspirin intolerance. Conclusion Although the presence of TNFα-1031 and TNFβ+252 gene polymorphisms did not render any significant associations between CRS and healthy control, this study suggests that TNFα-1031 gene polymorphisms in CRS patients with atopy may be associated with increase susceptibility towards CRS.http://www.e-ceo.org/upload/pdf/ceo-2016-01732.pdfRhinosinusitisTumour Necrosis FactorsSingle Nucleotide Polymorphism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Khairunnisak Misron Suzina Sheikh Ab Hamid Azlina Ahmad Ramiza Ramza Ramli |
spellingShingle |
Khairunnisak Misron Suzina Sheikh Ab Hamid Azlina Ahmad Ramiza Ramza Ramli A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic Rhinosinusitis Clinical and Experimental Otorhinolaryngology Rhinosinusitis Tumour Necrosis Factors Single Nucleotide Polymorphism |
author_facet |
Khairunnisak Misron Suzina Sheikh Ab Hamid Azlina Ahmad Ramiza Ramza Ramli |
author_sort |
Khairunnisak Misron |
title |
A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic Rhinosinusitis |
title_short |
A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic Rhinosinusitis |
title_full |
A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic Rhinosinusitis |
title_fullStr |
A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic Rhinosinusitis |
title_full_unstemmed |
A Study of Single Nucleotide Polymorphisms of Tumour Necrosis Factor α-1031 And Tumour Necrosis Factor β+ 252 in Chronic Rhinosinusitis |
title_sort |
study of single nucleotide polymorphisms of tumour necrosis factor α-1031 and tumour necrosis factor β+ 252 in chronic rhinosinusitis |
publisher |
Korean Society of Otorhinolaryngology-Head and Neck Surgery |
series |
Clinical and Experimental Otorhinolaryngology |
issn |
1976-8710 2005-0720 |
publishDate |
2017-09-01 |
description |
Objectives This case-controlled study aimed to identify the association of tumor necrosis factor (TNF)α-1031 and TNFβ+ 252 gene polymorphisms between chronic rhinosinusitis (CRS) and healthy controls. Another purpose of this study was to investigate the associations of these gene polymorphisms with factors related to CRS. Methods All deoxyribonucleic acid (DNA) samples were genotyped for TNFα-1031 and TNFβ+252 genes by mean of polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP). The statistical analysis were carried out using chi-square test or Fisher exact test to determine the associations of these gene polymorphisms in CRS. Multiple logistic regression was performed to evaluate the associations of these gene polymorphisms in CRS and its related risk factors. Results The genotype and allele frequencies of TNFα-1031 and TNFβ+252 gene did not show any significant associations between CRS and healthy controls. However, a significantly statistical difference of TNFα-1031 was observed in CRS participants with atopy (P-value, 0.045; odds ratio, 3.66) but not in CRS with asthma or aspirin intolerance. Conclusion Although the presence of TNFα-1031 and TNFβ+252 gene polymorphisms did not render any significant associations between CRS and healthy control, this study suggests that TNFα-1031 gene polymorphisms in CRS patients with atopy may be associated with increase susceptibility towards CRS. |
topic |
Rhinosinusitis Tumour Necrosis Factors Single Nucleotide Polymorphism |
url |
http://www.e-ceo.org/upload/pdf/ceo-2016-01732.pdf |
work_keys_str_mv |
AT khairunnisakmisron astudyofsinglenucleotidepolymorphismsoftumournecrosisfactora1031andtumournecrosisfactorb252inchronicrhinosinusitis AT suzinasheikhabhamid astudyofsinglenucleotidepolymorphismsoftumournecrosisfactora1031andtumournecrosisfactorb252inchronicrhinosinusitis AT azlinaahmad astudyofsinglenucleotidepolymorphismsoftumournecrosisfactora1031andtumournecrosisfactorb252inchronicrhinosinusitis AT ramizaramzaramli astudyofsinglenucleotidepolymorphismsoftumournecrosisfactora1031andtumournecrosisfactorb252inchronicrhinosinusitis AT khairunnisakmisron studyofsinglenucleotidepolymorphismsoftumournecrosisfactora1031andtumournecrosisfactorb252inchronicrhinosinusitis AT suzinasheikhabhamid studyofsinglenucleotidepolymorphismsoftumournecrosisfactora1031andtumournecrosisfactorb252inchronicrhinosinusitis AT azlinaahmad studyofsinglenucleotidepolymorphismsoftumournecrosisfactora1031andtumournecrosisfactorb252inchronicrhinosinusitis AT ramizaramzaramli studyofsinglenucleotidepolymorphismsoftumournecrosisfactora1031andtumournecrosisfactorb252inchronicrhinosinusitis |
_version_ |
1725445685469249536 |