Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, w...

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Main Authors: Jailson F. B. Querido, Jon Agirre, Gerardo A. Marti, Diego M. A. Guérin, Marcelo Sousa Silva
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2013/218593
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spelling doaj-233c8927daa64bacbd6dd4e20e359f742020-11-25T00:10:59ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/218593218593Molecular Techniques for Dicistrovirus Detection without RNA Extraction or PurificationJailson F. B. Querido0Jon Agirre1Gerardo A. Marti2Diego M. A. Guérin3Marcelo Sousa Silva4Centre for Malaria and Tropical Diseases, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, PortugalUnidad de Biofísica (UBF), CSIC-UPV/EHU, Barrio Sarriena S/N, Bizkaia, 48940 Leioa, SpainCentro de Estudios Parasitológicos y de Vectores (CEPAVE), CCT-La Plata/CONICET-UNLP, No. 584, 1900 La Plata, ArgentinaUnidad de Biofísica (UBF), CSIC-UPV/EHU, Barrio Sarriena S/N, Bizkaia, 48940 Leioa, SpainCentre for Malaria and Tropical Diseases, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, PortugalDicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.http://dx.doi.org/10.1155/2013/218593
collection DOAJ
language English
format Article
sources DOAJ
author Jailson F. B. Querido
Jon Agirre
Gerardo A. Marti
Diego M. A. Guérin
Marcelo Sousa Silva
spellingShingle Jailson F. B. Querido
Jon Agirre
Gerardo A. Marti
Diego M. A. Guérin
Marcelo Sousa Silva
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
BioMed Research International
author_facet Jailson F. B. Querido
Jon Agirre
Gerardo A. Marti
Diego M. A. Guérin
Marcelo Sousa Silva
author_sort Jailson F. B. Querido
title Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_short Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_full Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_fullStr Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_full_unstemmed Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_sort molecular techniques for dicistrovirus detection without rna extraction or purification
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2013-01-01
description Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
url http://dx.doi.org/10.1155/2013/218593
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