Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, w...
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doaj-233c8927daa64bacbd6dd4e20e359f742020-11-25T00:10:59ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/218593218593Molecular Techniques for Dicistrovirus Detection without RNA Extraction or PurificationJailson F. B. Querido0Jon Agirre1Gerardo A. Marti2Diego M. A. Guérin3Marcelo Sousa Silva4Centre for Malaria and Tropical Diseases, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, PortugalUnidad de Biofísica (UBF), CSIC-UPV/EHU, Barrio Sarriena S/N, Bizkaia, 48940 Leioa, SpainCentro de Estudios Parasitológicos y de Vectores (CEPAVE), CCT-La Plata/CONICET-UNLP, No. 584, 1900 La Plata, ArgentinaUnidad de Biofísica (UBF), CSIC-UPV/EHU, Barrio Sarriena S/N, Bizkaia, 48940 Leioa, SpainCentre for Malaria and Tropical Diseases, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, PortugalDicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.http://dx.doi.org/10.1155/2013/218593 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jailson F. B. Querido Jon Agirre Gerardo A. Marti Diego M. A. Guérin Marcelo Sousa Silva |
spellingShingle |
Jailson F. B. Querido Jon Agirre Gerardo A. Marti Diego M. A. Guérin Marcelo Sousa Silva Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification BioMed Research International |
author_facet |
Jailson F. B. Querido Jon Agirre Gerardo A. Marti Diego M. A. Guérin Marcelo Sousa Silva |
author_sort |
Jailson F. B. Querido |
title |
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification |
title_short |
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification |
title_full |
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification |
title_fullStr |
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification |
title_full_unstemmed |
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification |
title_sort |
molecular techniques for dicistrovirus detection without rna extraction or purification |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus. |
url |
http://dx.doi.org/10.1155/2013/218593 |
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