Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro
The nutritional benefits of pomegranate have attracted great scientific interest. The pomegranate, including the pomegranate peel, has been used worldwide for many years as a fruit with medicinal activity, mostly antioxidant properties. Among chronic diseases, osteoporosis, which is associated with...
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doaj-23462e9724564f5b8000a29c54c258ac2020-11-24T21:46:01ZengMDPI AGNutrients2072-66432015-11-017119265928410.3390/nu7115465nu7115465Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in VitroMélanie Spilmont0Laurent Léotoing1Marie-Jeanne Davicco2Patrice Lebecque3Elisabeth Miot-Noirault4Paul Pilet5Laurent Rios6Yohann Wittrant7Véronique Coxam8Unité de Nutrition Humaine, CRNH Auvergne, UMR 1019, INRA, F-63000 Clermont-Ferrand, FranceUnité de Nutrition Humaine, CRNH Auvergne, UMR 1019, INRA, F-63000 Clermont-Ferrand, FranceUnité de Nutrition Humaine, CRNH Auvergne, UMR 1019, INRA, F-63000 Clermont-Ferrand, FranceUnité de Nutrition Humaine, CRNH Auvergne, UMR 1019, INRA, F-63000 Clermont-Ferrand, FranceImagerie Moléculaire et Thérapie Vectorisée, Université d'Auvergne, Clermont Université, UMR 990, INSERM, BP 10448, F-63000 Clermont-Ferrand, FranceLaboratoire d'Ingénierie Ostéo-Articulaire et Dentaire-LIOAD, Université de Nantes, UMR 791, INSERM, F-44042 Nantes, FranceGREENTECH SA Biopôle Clermont-Limagne, F-63360 Saint-Beauzire, FranceUnité de Nutrition Humaine, CRNH Auvergne, UMR 1019, INRA, F-63000 Clermont-Ferrand, FranceUnité de Nutrition Humaine, CRNH Auvergne, UMR 1019, INRA, F-63000 Clermont-Ferrand, FranceThe nutritional benefits of pomegranate have attracted great scientific interest. The pomegranate, including the pomegranate peel, has been used worldwide for many years as a fruit with medicinal activity, mostly antioxidant properties. Among chronic diseases, osteoporosis, which is associated with bone remodelling impairment leading to progressive bone loss, could eventually benefit from antioxidant compounds because of the involvement of oxidative stress in the pathogenesis of osteopenia. In this study, with in vivo and ex vivo experiments, we investigated whether the consumption of pomegranate peel extract (PGPE) could limit the process of osteopenia. We demonstrated that in ovariectomized (OVX) C57BL/6J mice, PGPE consumption was able to significantly prevent the decrease in bone mineral density (−31.9%; p < 0.001 vs. OVX mice) and bone microarchitecture impairment. Moreover, the exposure of RAW264.7 cells to serum harvested from mice that had been given a PGPE-enriched diet elicited reduced osteoclast differentiation and bone resorption, as shown by the inhibition of the major osteoclast markers. In addition, PGPE appeared to substantially stimulate osteoblastic MC3T3-E1 alkaline phosphatase (ALP) activity at day 7, mineralization at day 21 and the transcription level of osteogenic markers. PGPE may be effective in preventing the bone loss associated with ovariectomy in mice, and offers a promising alternative for the nutritional management of this disease.http://www.mdpi.com/2072-6643/7/11/5465pomegranate peel extractnutritional preventionbone healthcell linesanimal model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mélanie Spilmont Laurent Léotoing Marie-Jeanne Davicco Patrice Lebecque Elisabeth Miot-Noirault Paul Pilet Laurent Rios Yohann Wittrant Véronique Coxam |
spellingShingle |
Mélanie Spilmont Laurent Léotoing Marie-Jeanne Davicco Patrice Lebecque Elisabeth Miot-Noirault Paul Pilet Laurent Rios Yohann Wittrant Véronique Coxam Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro Nutrients pomegranate peel extract nutritional prevention bone health cell lines animal model |
author_facet |
Mélanie Spilmont Laurent Léotoing Marie-Jeanne Davicco Patrice Lebecque Elisabeth Miot-Noirault Paul Pilet Laurent Rios Yohann Wittrant Véronique Coxam |
author_sort |
Mélanie Spilmont |
title |
Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro |
title_short |
Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro |
title_full |
Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro |
title_fullStr |
Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro |
title_full_unstemmed |
Pomegranate Peel Extract Prevents Bone Loss in a Preclinical Model of Osteoporosis and Stimulates Osteoblastic Differentiation in Vitro |
title_sort |
pomegranate peel extract prevents bone loss in a preclinical model of osteoporosis and stimulates osteoblastic differentiation in vitro |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2015-11-01 |
description |
The nutritional benefits of pomegranate have attracted great scientific interest. The pomegranate, including the pomegranate peel, has been used worldwide for many years as a fruit with medicinal activity, mostly antioxidant properties. Among chronic diseases, osteoporosis, which is associated with bone remodelling impairment leading to progressive bone loss, could eventually benefit from antioxidant compounds because of the involvement of oxidative stress in the pathogenesis of osteopenia. In this study, with in vivo and ex vivo experiments, we investigated whether the consumption of pomegranate peel extract (PGPE) could limit the process of osteopenia. We demonstrated that in ovariectomized (OVX) C57BL/6J mice, PGPE consumption was able to significantly prevent the decrease in bone mineral density (−31.9%; p < 0.001 vs. OVX mice) and bone microarchitecture impairment. Moreover, the exposure of RAW264.7 cells to serum harvested from mice that had been given a PGPE-enriched diet elicited reduced osteoclast differentiation and bone resorption, as shown by the inhibition of the major osteoclast markers. In addition, PGPE appeared to substantially stimulate osteoblastic MC3T3-E1 alkaline phosphatase (ALP) activity at day 7, mineralization at day 21 and the transcription level of osteogenic markers. PGPE may be effective in preventing the bone loss associated with ovariectomy in mice, and offers a promising alternative for the nutritional management of this disease. |
topic |
pomegranate peel extract nutritional prevention bone health cell lines animal model |
url |
http://www.mdpi.com/2072-6643/7/11/5465 |
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