Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds
Defining the most adequate architecture of a bone substitute scaffold is a topic that has received much attention over the last 40 years. However, contradictory results exist on the effect of grain size and microporosity. Therefore, the aim of this study was to determine the effect of these two fact...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
AO Research Institute Davos
2014-10-01
|
Series: | European Cells & Materials |
Subjects: | |
Online Access: | http://www.ecmjournal.org/journal/papers/vol028/pdf/v028a21.pdf |
id |
doaj-2355034a36d2496fb0a4c61996c5f303 |
---|---|
record_format |
Article |
spelling |
doaj-2355034a36d2496fb0a4c61996c5f3032020-11-24T23:55:38Zeng AO Research Institute DavosEuropean Cells & Materials1473-22622014-10-0128299319Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffoldsH LapczynaL GaleaS WüstM Bohner0S JerbanA SweedyN DoebelinN van GarderenS HofmannG BaroudR MüllerB von RechenbergRMS Foundation, Bischmattstrasse 12, CH-2544 Bettlach, SwitzerlandDefining the most adequate architecture of a bone substitute scaffold is a topic that has received much attention over the last 40 years. However, contradictory results exist on the effect of grain size and microporosity. Therefore, the aim of this study was to determine the effect of these two factors on the in vivo behaviour of β-tricalcium phosphate (β-TCP) scaffolds. For that purpose, β-TCP scaffolds were produced with roughly the same macropore size (≈ 150 μm), and porosity (≈ 80 %), but two levels of microporosity (low: 10 % / high: ≈ 25 %) and grain size (small: 1.3 μm /large: ≈ 3.3 μm). The sample architecture was characterised extensively using materialography, Hg porosimetry, micro-computed tomography (μCT), and nitrogen adsorption. The scaffolds were implanted for 2, 4 and 8 weeks in a cylindrical 5-wall cancellous bone defect in sheep. The histological, histomorphometrical and μCT analysis of the samples revealed that all four scaffold types were almost completely resorbed within 8 weeks and replaced by new bone. Despite the three-fold difference in microporosity and grain size, very few biological differences were observed. The only significant effect at p < 0.01 was a slightly faster resorption rate and soft tissue formation between 4 and 8 weeks of implantation when microporosity was increased. Past and present results suggest that the biological response of this particular defect is not very sensitive towards physico-chemical differences of resorbable bone graft substitutes. As bone formed not only in the macropores but also in the micropores, a closer study at the microscopic and localised effects is necessary.http://www.ecmjournal.org/journal/papers/vol028/pdf/v028a21.pdfResorptionbone graftcalcium phosphatemicrostructureporosityscaffoldtricalcium phosphatemicroporegrainsize |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
H Lapczyna L Galea S Wüst M Bohner S Jerban A Sweedy N Doebelin N van Garderen S Hofmann G Baroud R Müller B von Rechenberg |
spellingShingle |
H Lapczyna L Galea S Wüst M Bohner S Jerban A Sweedy N Doebelin N van Garderen S Hofmann G Baroud R Müller B von Rechenberg Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds European Cells & Materials Resorption bone graft calcium phosphate microstructure porosity scaffold tricalcium phosphate micropore grain size |
author_facet |
H Lapczyna L Galea S Wüst M Bohner S Jerban A Sweedy N Doebelin N van Garderen S Hofmann G Baroud R Müller B von Rechenberg |
author_sort |
H Lapczyna |
title |
Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds |
title_short |
Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds |
title_full |
Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds |
title_fullStr |
Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds |
title_full_unstemmed |
Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds |
title_sort |
effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds |
publisher |
AO Research Institute Davos |
series |
European Cells & Materials |
issn |
1473-2262 |
publishDate |
2014-10-01 |
description |
Defining the most adequate architecture of a bone substitute scaffold is a topic that has received much attention over the last 40 years. However, contradictory results exist on the effect of grain size and microporosity. Therefore, the aim of this study was to determine the effect of these two factors on the in vivo behaviour of β-tricalcium phosphate (β-TCP) scaffolds. For that purpose, β-TCP scaffolds were produced with roughly the same macropore size (≈ 150 μm), and porosity (≈ 80 %), but two levels of microporosity (low: 10 % / high: ≈ 25 %) and grain size (small: 1.3 μm /large: ≈ 3.3 μm). The sample architecture was characterised extensively using materialography, Hg porosimetry, micro-computed tomography (μCT), and nitrogen adsorption. The scaffolds were implanted for 2, 4 and 8 weeks in a cylindrical 5-wall cancellous bone defect in sheep. The histological, histomorphometrical and μCT analysis of the samples revealed that all four scaffold types were almost completely resorbed within 8 weeks and replaced by new bone. Despite the three-fold difference in microporosity and grain size, very few biological differences were observed. The only significant effect at p < 0.01 was a slightly faster resorption rate and soft tissue formation between 4 and 8 weeks of implantation when microporosity was increased. Past and present results suggest that the biological response of this particular defect is not very sensitive towards physico-chemical differences of resorbable bone graft substitutes. As bone formed not only in the macropores but also in the micropores, a closer study at the microscopic and localised effects is necessary. |
topic |
Resorption bone graft calcium phosphate microstructure porosity scaffold tricalcium phosphate micropore grain size |
url |
http://www.ecmjournal.org/journal/papers/vol028/pdf/v028a21.pdf |
work_keys_str_mv |
AT hlapczyna effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT lgalea effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT swust effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT mbohner effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT sjerban effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT asweedy effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT ndoebelin effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT nvangarderen effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT shofmann effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT gbaroud effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT rmuller effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds AT bvonrechenberg effectofgrainsizeandmicroporosityontheinvivobehaviourofbtricalciumphosphatescaffolds |
_version_ |
1725461356605341696 |