High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo
Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in ch...
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doaj-23552d414d2440e4a3ff55fdfd96d3042020-11-25T02:15:41ZengMDPI AGViruses1999-49152019-10-01111092610.3390/v11100926v11100926High Resolution Analysis of Respiratory Syncytial Virus Infection In VivoWaleed Aljabr0Stuart Armstrong1Natasha Y. Rickett2Georgios Pollakis3Olivier Touzelet4Elaine Cloutman-Green5David A. Matthews6Julian A. Hiscox7King Fahad Medical City, Research Center, 59046 Riyadh 11525, Saudi ArabiaInstitute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UKInstitute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UKInstitute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UKSchool of Medicine, Dentistry & Biomedical Sciences, Queen’s University Belfast, Belfast BT9 7BL, UKGreat Ormond Street Hospital, London WC1N 3JH, UKSchool of Medical Sciences, University of Bristol, Bristol BS8 1TD, UKInstitute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UKHuman respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in children, nasopharyngeal aspirates were taken from children with different viral loads and a combined high throughput RNAseq and label free quantitative proteomics approach was used to characterize the nucleic acid and proteins in these samples. HRSV proteins were identified in the nasopharyngeal aspirates from infected children, and their abundance correlated with viral load (Ct value), confirming HRSV infection. Analysis of the HRSV genome indicated that the children were infected with sub-group A virus and that minor variants in nucleotide frequency occurred in discrete clusters along the HRSV genome, and within a patient clustered distinctly within the glycoprotein gene. Data from the samples were binned into four groups; no-HRSV infection (control), high viral load (Ct < 20), medium viral load (Ct = 20−25), and low viral load (Ct > 25). Cellular proteins associated with the anti-viral response (e.g., ISG15) were identified in the nasopharyngeal aspirates and their abundance was correlated with viral load. These combined approaches have not been used before to study HRSV biology in vivo and can be readily applied to the study the variation of virus host interactions.https://www.mdpi.com/1999-4915/11/10/926respiratory syncytial virusproteomicsrnaseqnasopharyngeal aspiratehost responsequasispeciesclinical samplerespiratory disease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Waleed Aljabr Stuart Armstrong Natasha Y. Rickett Georgios Pollakis Olivier Touzelet Elaine Cloutman-Green David A. Matthews Julian A. Hiscox |
spellingShingle |
Waleed Aljabr Stuart Armstrong Natasha Y. Rickett Georgios Pollakis Olivier Touzelet Elaine Cloutman-Green David A. Matthews Julian A. Hiscox High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo Viruses respiratory syncytial virus proteomics rnaseq nasopharyngeal aspirate host response quasispecies clinical sample respiratory disease |
author_facet |
Waleed Aljabr Stuart Armstrong Natasha Y. Rickett Georgios Pollakis Olivier Touzelet Elaine Cloutman-Green David A. Matthews Julian A. Hiscox |
author_sort |
Waleed Aljabr |
title |
High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo |
title_short |
High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo |
title_full |
High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo |
title_fullStr |
High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo |
title_full_unstemmed |
High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo |
title_sort |
high resolution analysis of respiratory syncytial virus infection in vivo |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2019-10-01 |
description |
Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in children, nasopharyngeal aspirates were taken from children with different viral loads and a combined high throughput RNAseq and label free quantitative proteomics approach was used to characterize the nucleic acid and proteins in these samples. HRSV proteins were identified in the nasopharyngeal aspirates from infected children, and their abundance correlated with viral load (Ct value), confirming HRSV infection. Analysis of the HRSV genome indicated that the children were infected with sub-group A virus and that minor variants in nucleotide frequency occurred in discrete clusters along the HRSV genome, and within a patient clustered distinctly within the glycoprotein gene. Data from the samples were binned into four groups; no-HRSV infection (control), high viral load (Ct < 20), medium viral load (Ct = 20−25), and low viral load (Ct > 25). Cellular proteins associated with the anti-viral response (e.g., ISG15) were identified in the nasopharyngeal aspirates and their abundance was correlated with viral load. These combined approaches have not been used before to study HRSV biology in vivo and can be readily applied to the study the variation of virus host interactions. |
topic |
respiratory syncytial virus proteomics rnaseq nasopharyngeal aspirate host response quasispecies clinical sample respiratory disease |
url |
https://www.mdpi.com/1999-4915/11/10/926 |
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