Comparative analysis of gut microbiota among the male, female and pregnant giant pandas (Ailuropoda Melanoleuca)
The giant panda (GP) was the most endangered species in China, and gut microbiota plays a vital role in host health. To determine the differences of the gut microbiota among the male, female and pregnant GPs, a comparative analysis of gut microbiota in GPs was carried out by 16S rRNA and ITS high-th...
Main Authors: | , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
De Gruyter
2019-07-01
|
Series: | Open Life Sciences |
Subjects: | |
Online Access: | https://doi.org/10.1515/biol-2019-0032 |
id |
doaj-23605cfe8dc144418f86ab5c7f55c061 |
---|---|
record_format |
Article |
spelling |
doaj-23605cfe8dc144418f86ab5c7f55c0612021-09-05T20:42:23ZengDe GruyterOpen Life Sciences2391-54122019-07-0114128829810.1515/biol-2019-0032biol-2019-0032Comparative analysis of gut microbiota among the male, female and pregnant giant pandas (Ailuropoda Melanoleuca)Zhao Siyue0Li Caiwu1Li Guo2Yang Shengzhi3Zhou Yingming4He Yongguo5Wu Daifu6Zhou Yu7Zeng Wen8Li Ti9Qu Yuanyuan10Li Bei11Deng Wenwen12Jin Lei13Yu Xiumei14Huang Yan15Zhang Hemin16Zou Likou17Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaDepartment of Applied Microbiology, College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaDepartment of Applied Microbiology, College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, ChinaDepartment of Applied Microbiology, College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, ChinaDepartment of Applied Microbiology, College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, ChinaDepartment of Applied Microbiology, College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaChina Conservation and Research Center for Giant Panda, 611830, Dujiangyan, Sichuan, ChinaDepartment of Applied Microbiology, College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, ChinaThe giant panda (GP) was the most endangered species in China, and gut microbiota plays a vital role in host health. To determine the differences of the gut microbiota among the male, female and pregnant GPs, a comparative analysis of gut microbiota in GPs was carried out by 16S rRNA and ITS high-throughput sequencing. In 16S rRNA sequencing, 435 OTUs, 17 phyla and 182 genera were totally detected. Firmicutes (53.6%) was the predominant phylum followed by Proteobacteria (37.8%) and Fusobacteria (7.1%). Escherichia/Shigella (35.9%) was the most prevalent genus followed by Streptococcus (25.9%) and Clostridium (11.1%). In ITS sequencing, 920 OTUs, 6 phyla and 322 genera were also detected. Ascomycota (71.3%) was the predominant phylum followed by Basidiomycota (28.4%) and Zygomycota (0.15%). Purpureocillium (4.4%) was the most prevalent genus followed by Cladosporium (2.5%) and Pezicula (2.4%). Comparative analysis indicated that the male GPs harbor a higher abundance of phylum Firmicutes than female GPs with the contribution from genus Streptococcus. Meanwhile, the female GPs harbor a higher abundance of phylum Proteobacteria than male GPs with the contribution from genus Escherichia/ Shigella. In addition, the shift in bacteria from female to pregnant GPs indicated that phylum Firmicutes increased significantly with the contribution from Clostridium in the gut, which may provide an opportunity to study possible associations with low reproduction of the GPs.https://doi.org/10.1515/biol-2019-0032giant pandagut microbiotahigh-throughput sequencinggenderpregnant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhao Siyue Li Caiwu Li Guo Yang Shengzhi Zhou Yingming He Yongguo Wu Daifu Zhou Yu Zeng Wen Li Ti Qu Yuanyuan Li Bei Deng Wenwen Jin Lei Yu Xiumei Huang Yan Zhang Hemin Zou Likou |
spellingShingle |
Zhao Siyue Li Caiwu Li Guo Yang Shengzhi Zhou Yingming He Yongguo Wu Daifu Zhou Yu Zeng Wen Li Ti Qu Yuanyuan Li Bei Deng Wenwen Jin Lei Yu Xiumei Huang Yan Zhang Hemin Zou Likou Comparative analysis of gut microbiota among the male, female and pregnant giant pandas (Ailuropoda Melanoleuca) Open Life Sciences giant panda gut microbiota high-throughput sequencing gender pregnant |
author_facet |
Zhao Siyue Li Caiwu Li Guo Yang Shengzhi Zhou Yingming He Yongguo Wu Daifu Zhou Yu Zeng Wen Li Ti Qu Yuanyuan Li Bei Deng Wenwen Jin Lei Yu Xiumei Huang Yan Zhang Hemin Zou Likou |
author_sort |
Zhao Siyue |
title |
Comparative analysis of gut microbiota among the male, female and pregnant giant pandas (Ailuropoda Melanoleuca) |
title_short |
Comparative analysis of gut microbiota among the male, female and pregnant giant pandas (Ailuropoda Melanoleuca) |
title_full |
Comparative analysis of gut microbiota among the male, female and pregnant giant pandas (Ailuropoda Melanoleuca) |
title_fullStr |
Comparative analysis of gut microbiota among the male, female and pregnant giant pandas (Ailuropoda Melanoleuca) |
title_full_unstemmed |
Comparative analysis of gut microbiota among the male, female and pregnant giant pandas (Ailuropoda Melanoleuca) |
title_sort |
comparative analysis of gut microbiota among the male, female and pregnant giant pandas (ailuropoda melanoleuca) |
publisher |
De Gruyter |
series |
Open Life Sciences |
issn |
2391-5412 |
publishDate |
2019-07-01 |
description |
The giant panda (GP) was the most endangered species in China, and gut microbiota plays a vital role in host health. To determine the differences of the gut microbiota among the male, female and pregnant GPs, a comparative analysis of gut microbiota in GPs was carried out by 16S rRNA and ITS high-throughput sequencing. In 16S rRNA sequencing, 435 OTUs, 17 phyla and 182 genera were totally detected. Firmicutes (53.6%) was the predominant phylum followed by Proteobacteria (37.8%) and Fusobacteria (7.1%). Escherichia/Shigella (35.9%) was the most prevalent genus followed by Streptococcus (25.9%) and Clostridium (11.1%). In ITS sequencing, 920 OTUs, 6 phyla and 322 genera were also detected. Ascomycota (71.3%) was the predominant phylum followed by Basidiomycota (28.4%) and Zygomycota (0.15%). Purpureocillium (4.4%) was the most prevalent genus followed by Cladosporium (2.5%) and Pezicula (2.4%). Comparative analysis indicated that the male GPs harbor a higher abundance of phylum Firmicutes than female GPs with the contribution from genus Streptococcus. Meanwhile, the female GPs harbor a higher abundance of phylum Proteobacteria than male GPs with the contribution from genus Escherichia/ Shigella. In addition, the shift in bacteria from female to pregnant GPs indicated that phylum Firmicutes increased significantly with the contribution from Clostridium in the gut, which may provide an opportunity to study possible associations with low reproduction of the GPs. |
topic |
giant panda gut microbiota high-throughput sequencing gender pregnant |
url |
https://doi.org/10.1515/biol-2019-0032 |
work_keys_str_mv |
AT zhaosiyue comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT licaiwu comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT liguo comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT yangshengzhi comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT zhouyingming comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT heyongguo comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT wudaifu comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT zhouyu comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT zengwen comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT liti comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT quyuanyuan comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT libei comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT dengwenwen comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT jinlei comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT yuxiumei comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT huangyan comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT zhanghemin comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca AT zoulikou comparativeanalysisofgutmicrobiotaamongthemalefemaleandpregnantgiantpandasailuropodamelanoleuca |
_version_ |
1717785706411065344 |