Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats
Study DesignThis study investigated the effects of transplantation of alginate encapsulated neural stem cells (NSCs) on spinal cord injury in Sprague-Dawley male rats. The neurological functions were assessed for 6 weeks after transplantation along with a histological study and measurement of caspas...
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doaj-239b8b0d526744fc8c6cbd95ffe4a5e72020-11-24T22:31:24ZengKorean Spine SocietyAsian Spine Journal1976-19021976-78462016-08-0110461161810.4184/asj.2016.10.4.611126Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in RatsSeyed Mojtaba Hosseini0Ali Sharafkhah1Omid Koohi-Hosseinabadi2Maryam Semsar-Kazerooni3Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.Study DesignThis study investigated the effects of transplantation of alginate encapsulated neural stem cells (NSCs) on spinal cord injury in Sprague-Dawley male rats. The neurological functions were assessed for 6 weeks after transplantation along with a histological study and measurement of caspase-3 levels.PurposeThe aim of this study was to discover whether NSCs cultured in alginate transplantation improve recovery from spinal cord injury.Overview of LiteratureSpinal cord injury is one of the leading causes of disability and it has no effective treatment. Spinal cord injury can also cause sensory impairment. With an impetus on using stem cells therapy in various central nervous system settings, there is an interest in using stem cells for addressing spinal cord injury. Neural stem cell is one type of stem cells that is able to differentiate to all three neural lineages and it shows promise in spinal injury treatment. Furthermore, a number of studies have shown that culturing NSCs in three-dimensional (3D) scaffolds like alginate could enhance neural differentiation.MethodsThe NSCs were isolated from 14-day-old rat embryos. The isolated NSCs were cultured in growth media containing basic fibroblast growth factor and endothelial growth factor. The cells were characterized by differentiating to three neural lineages and they were cultured in an alginate scaffold. After 7 days the cells were encapsulated and transplanted in a rat model of spinal cord injury.ResultsOur data showed that culturing in an alginate 3D scaffold and transplantation of the NSCs could improve neurological outcome in a rat model of spinal cord injury. The inflammation scores and lesion sizes and also the activity of caspase-3 (for apoptosis evaluation) were less in encapsulated neural stem cell transplantation cases.ConclusionsTransplantation of NSCs that were cultured in an alginate scaffold led to a better clinical and histological outcome for recovery from spinal cord injury in a rat model.http://www.asianspinejournal.org/upload/pdf/asj-10-611.pdfNeural stem cellsAlginateSpinal cord injuries |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seyed Mojtaba Hosseini Ali Sharafkhah Omid Koohi-Hosseinabadi Maryam Semsar-Kazerooni |
spellingShingle |
Seyed Mojtaba Hosseini Ali Sharafkhah Omid Koohi-Hosseinabadi Maryam Semsar-Kazerooni Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats Asian Spine Journal Neural stem cells Alginate Spinal cord injuries |
author_facet |
Seyed Mojtaba Hosseini Ali Sharafkhah Omid Koohi-Hosseinabadi Maryam Semsar-Kazerooni |
author_sort |
Seyed Mojtaba Hosseini |
title |
Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats |
title_short |
Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats |
title_full |
Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats |
title_fullStr |
Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats |
title_full_unstemmed |
Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats |
title_sort |
transplantation of neural stem cells cultured in alginate scaffold for spinal cord injury in rats |
publisher |
Korean Spine Society |
series |
Asian Spine Journal |
issn |
1976-1902 1976-7846 |
publishDate |
2016-08-01 |
description |
Study DesignThis study investigated the effects of transplantation of alginate encapsulated neural stem cells (NSCs) on spinal cord injury in Sprague-Dawley male rats. The neurological functions were assessed for 6 weeks after transplantation along with a histological study and measurement of caspase-3 levels.PurposeThe aim of this study was to discover whether NSCs cultured in alginate transplantation improve recovery from spinal cord injury.Overview of LiteratureSpinal cord injury is one of the leading causes of disability and it has no effective treatment. Spinal cord injury can also cause sensory impairment. With an impetus on using stem cells therapy in various central nervous system settings, there is an interest in using stem cells for addressing spinal cord injury. Neural stem cell is one type of stem cells that is able to differentiate to all three neural lineages and it shows promise in spinal injury treatment. Furthermore, a number of studies have shown that culturing NSCs in three-dimensional (3D) scaffolds like alginate could enhance neural differentiation.MethodsThe NSCs were isolated from 14-day-old rat embryos. The isolated NSCs were cultured in growth media containing basic fibroblast growth factor and endothelial growth factor. The cells were characterized by differentiating to three neural lineages and they were cultured in an alginate scaffold. After 7 days the cells were encapsulated and transplanted in a rat model of spinal cord injury.ResultsOur data showed that culturing in an alginate 3D scaffold and transplantation of the NSCs could improve neurological outcome in a rat model of spinal cord injury. The inflammation scores and lesion sizes and also the activity of caspase-3 (for apoptosis evaluation) were less in encapsulated neural stem cell transplantation cases.ConclusionsTransplantation of NSCs that were cultured in an alginate scaffold led to a better clinical and histological outcome for recovery from spinal cord injury in a rat model. |
topic |
Neural stem cells Alginate Spinal cord injuries |
url |
http://www.asianspinejournal.org/upload/pdf/asj-10-611.pdf |
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