Biological and Pathological Studies of Rosmarinic Acid as an Inhibitor of Hemorrhagic Trimeresurus flavoviridis (habu) Venom
In our previous report, rosmarinic acid (RA) was revealed to be an antidote active compound in Argusia argentea (family: Boraginaceae). The plant is locally used in Okinawa in Japan as an antidote for poisoning from snake venom, Trimeresurus flavoviridis (habu). This article presents mechanistic evi...
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2010-10-01
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doaj-239d4c24deb4453fb424f5f0b06437812020-11-25T00:05:30ZengMDPI AGToxins2072-66512010-10-012102478248910.3390/toxins2102478Biological and Pathological Studies of Rosmarinic Acid as an Inhibitor of Hemorrhagic Trimeresurus flavoviridis (habu) VenomMasatake NiwaYoshiaki TakayaTsunemasa NonogakiToshiaki NikaiYumiko KomoriHnin Thanda AungIn our previous report, rosmarinic acid (RA) was revealed to be an antidote active compound in Argusia argentea (family: Boraginaceae). The plant is locally used in Okinawa in Japan as an antidote for poisoning from snake venom, Trimeresurus flavoviridis (habu). This article presents mechanistic evidence of RA’s neutralization of the hemorrhagic effects of snake venom. Anti-hemorrhagic activity was assayed by using several kinds of snake venom. Inhibition against fibrinogen hydrolytic and collagen hydrolytic activities of T. flavoviridis venom were examined by SDS-PAGE. A histopathological study was done by microscopy after administration of venom in the presence or absence of RA. RA was found to markedly neutralize venom-induced hemorrhage, fibrinogenolysis, cytotoxicity and digestion of type IV collagen activity. Moreover, RA inhibited both hemorrhage and neutrophil infiltrations caused by T. flavoviridis venom in pathology sections. These results demonstrate that RA inhibited most of the hemorrhage effects of venom. These findings indicate that rosmarinic acid can be expected to provide therapeutic benefits in neutralization of snake venom accompanied by heat stability. http://www.mdpi.com/2072-6651/2/10/2478/rosmarinic acidsnake venomhemorrhagemetalloproteinaseArgusia argentea |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Masatake Niwa Yoshiaki Takaya Tsunemasa Nonogaki Toshiaki Nikai Yumiko Komori Hnin Thanda Aung |
spellingShingle |
Masatake Niwa Yoshiaki Takaya Tsunemasa Nonogaki Toshiaki Nikai Yumiko Komori Hnin Thanda Aung Biological and Pathological Studies of Rosmarinic Acid as an Inhibitor of Hemorrhagic Trimeresurus flavoviridis (habu) Venom Toxins rosmarinic acid snake venom hemorrhage metalloproteinase Argusia argentea |
author_facet |
Masatake Niwa Yoshiaki Takaya Tsunemasa Nonogaki Toshiaki Nikai Yumiko Komori Hnin Thanda Aung |
author_sort |
Masatake Niwa |
title |
Biological and Pathological Studies of Rosmarinic Acid as an Inhibitor of Hemorrhagic Trimeresurus flavoviridis (habu) Venom |
title_short |
Biological and Pathological Studies of Rosmarinic Acid as an Inhibitor of Hemorrhagic Trimeresurus flavoviridis (habu) Venom |
title_full |
Biological and Pathological Studies of Rosmarinic Acid as an Inhibitor of Hemorrhagic Trimeresurus flavoviridis (habu) Venom |
title_fullStr |
Biological and Pathological Studies of Rosmarinic Acid as an Inhibitor of Hemorrhagic Trimeresurus flavoviridis (habu) Venom |
title_full_unstemmed |
Biological and Pathological Studies of Rosmarinic Acid as an Inhibitor of Hemorrhagic Trimeresurus flavoviridis (habu) Venom |
title_sort |
biological and pathological studies of rosmarinic acid as an inhibitor of hemorrhagic trimeresurus flavoviridis (habu) venom |
publisher |
MDPI AG |
series |
Toxins |
issn |
2072-6651 |
publishDate |
2010-10-01 |
description |
In our previous report, rosmarinic acid (RA) was revealed to be an antidote active compound in Argusia argentea (family: Boraginaceae). The plant is locally used in Okinawa in Japan as an antidote for poisoning from snake venom, Trimeresurus flavoviridis (habu). This article presents mechanistic evidence of RA’s neutralization of the hemorrhagic effects of snake venom. Anti-hemorrhagic activity was assayed by using several kinds of snake venom. Inhibition against fibrinogen hydrolytic and collagen hydrolytic activities of T. flavoviridis venom were examined by SDS-PAGE. A histopathological study was done by microscopy after administration of venom in the presence or absence of RA. RA was found to markedly neutralize venom-induced hemorrhage, fibrinogenolysis, cytotoxicity and digestion of type IV collagen activity. Moreover, RA inhibited both hemorrhage and neutrophil infiltrations caused by T. flavoviridis venom in pathology sections. These results demonstrate that RA inhibited most of the hemorrhage effects of venom. These findings indicate that rosmarinic acid can be expected to provide therapeutic benefits in neutralization of snake venom accompanied by heat stability. |
topic |
rosmarinic acid snake venom hemorrhage metalloproteinase Argusia argentea |
url |
http://www.mdpi.com/2072-6651/2/10/2478/ |
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