Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006
Streptococcus agalactiae is a common agent of clinical and subclinical bovine mastitis and an important cause of human infections, mainly among pregnant women, neonates and nonpregnant adults with underlying diseases. The present study describes the genetic and phenotypic diversity among 392 S. agal...
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doaj-23a070b0ae2c4a2585b27bff5e1b097b2020-11-25T04:00:13ZengElsevierBrazilian Journal of Infectious Diseases1678-439117213113610.1016/j.bjid.2012.09.006S1413-86702013000200003Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006Tatiana Castro Abreu Pinto0Natália Silva Costa1Aline Rosa Vianna Souza2Ligia Guedes da Silva3Ana Beatriz de Almeida Corrêa4Flavio Gimenis Fernandes5Ivi Cristina Menezes Oliveira6Marcos Corrêa de Mattos7Alexandre Soares Rosado8Leslie Claude Benchetrit9Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroStreptococcus agalactiae is a common agent of clinical and subclinical bovine mastitis and an important cause of human infections, mainly among pregnant women, neonates and nonpregnant adults with underlying diseases. The present study describes the genetic and phenotypic diversity among 392 S. agalactiae human and bovine strains isolated between 1980 and 2006 in Brazil. The most prevalent serotypes were Ia, II, III and V and all the strains were susceptible to penicillin, vancomycin and levofloxacin. Resistance to clindamycin, chloramphenicol, erythromycin, rifampicin and tetracycline was observed. Among the erythromycin resistant strains, mefA/E, ermA and, mainly, ermB gene were detected, and a shift of prevalence from the macrolide resistance phenotype to the macrolidelincosamide- streptogramin B resistance phenotype over the years was observed. The 23 macrolide-resistant strains showed 19 different pulsed-field gel electrophoresis profiles. Regarding macrolide resistance, a major concern in S. agalactiae epidemiology, the present study describes an increase in erythromycin resistance from the 80s to the 90s followed by a decrease in the 2000-2006 period. Also, the genetic heterogeneity described points out that erythromycin resistance in Brazil is rather due to horizontal gene transmission than to spreading of specific macrolide-resistant clones.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200003&lng=en&tlng=enStreptococcus agalactiaePulsed field gel electrophoresisEpidemiologySerotypingResistance profile |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tatiana Castro Abreu Pinto Natália Silva Costa Aline Rosa Vianna Souza Ligia Guedes da Silva Ana Beatriz de Almeida Corrêa Flavio Gimenis Fernandes Ivi Cristina Menezes Oliveira Marcos Corrêa de Mattos Alexandre Soares Rosado Leslie Claude Benchetrit |
spellingShingle |
Tatiana Castro Abreu Pinto Natália Silva Costa Aline Rosa Vianna Souza Ligia Guedes da Silva Ana Beatriz de Almeida Corrêa Flavio Gimenis Fernandes Ivi Cristina Menezes Oliveira Marcos Corrêa de Mattos Alexandre Soares Rosado Leslie Claude Benchetrit Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006 Brazilian Journal of Infectious Diseases Streptococcus agalactiae Pulsed field gel electrophoresis Epidemiology Serotyping Resistance profile |
author_facet |
Tatiana Castro Abreu Pinto Natália Silva Costa Aline Rosa Vianna Souza Ligia Guedes da Silva Ana Beatriz de Almeida Corrêa Flavio Gimenis Fernandes Ivi Cristina Menezes Oliveira Marcos Corrêa de Mattos Alexandre Soares Rosado Leslie Claude Benchetrit |
author_sort |
Tatiana Castro Abreu Pinto |
title |
Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006 |
title_short |
Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006 |
title_full |
Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006 |
title_fullStr |
Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006 |
title_full_unstemmed |
Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006 |
title_sort |
distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine streptococcus agalactiae strains isolated in brazil between 1980 and 2006 |
publisher |
Elsevier |
series |
Brazilian Journal of Infectious Diseases |
issn |
1678-4391 |
description |
Streptococcus agalactiae is a common agent of clinical and subclinical bovine mastitis and an important cause of human infections, mainly among pregnant women, neonates and nonpregnant adults with underlying diseases. The present study describes the genetic and phenotypic diversity among 392 S. agalactiae human and bovine strains isolated between 1980 and 2006 in Brazil. The most prevalent serotypes were Ia, II, III and V and all the strains were susceptible to penicillin, vancomycin and levofloxacin. Resistance to clindamycin, chloramphenicol, erythromycin, rifampicin and tetracycline was observed. Among the erythromycin resistant strains, mefA/E, ermA and, mainly, ermB gene were detected, and a shift of prevalence from the macrolide resistance phenotype to the macrolidelincosamide- streptogramin B resistance phenotype over the years was observed. The 23 macrolide-resistant strains showed 19 different pulsed-field gel electrophoresis profiles. Regarding macrolide resistance, a major concern in S. agalactiae epidemiology, the present study describes an increase in erythromycin resistance from the 80s to the 90s followed by a decrease in the 2000-2006 period. Also, the genetic heterogeneity described points out that erythromycin resistance in Brazil is rather due to horizontal gene transmission than to spreading of specific macrolide-resistant clones. |
topic |
Streptococcus agalactiae Pulsed field gel electrophoresis Epidemiology Serotyping Resistance profile |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200003&lng=en&tlng=en |
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