Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue
Increased plasminogen activator inhibitor-1 (PAI-1) levels are associated with a number of pathophysiological complications; among them is obesity. Resveratrol was proposed to improve obesity-related health problems, but the effect of resveratrol on PAI-1 gene expression in obesity is not completely...
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Online Access: | http://dx.doi.org/10.1155/2013/793525 |
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doaj-23a2c0b75e0d4cf2bff95e5238dfb7282020-11-24T22:02:18ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942013-01-01201310.1155/2013/793525793525Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose TissueIvana Zagotta0Elitsa Y. Dimova1Jan-Bernd Funcke2Martin Wabitsch3Thomas Kietzmann4Pamela Fischer-Posovszky5Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, Eythstraße 24, 89075 Ulm, GermanyDepartment of Biochemistry and Biocenter Oulu, University of Oulu, P.O.B. 3000, 90014 Oulu, FinlandDivision of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, Eythstraße 24, 89075 Ulm, GermanyDivision of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, Eythstraße 24, 89075 Ulm, GermanyDepartment of Biochemistry and Biocenter Oulu, University of Oulu, P.O.B. 3000, 90014 Oulu, FinlandDivision of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, Eythstraße 24, 89075 Ulm, GermanyIncreased plasminogen activator inhibitor-1 (PAI-1) levels are associated with a number of pathophysiological complications; among them is obesity. Resveratrol was proposed to improve obesity-related health problems, but the effect of resveratrol on PAI-1 gene expression in obesity is not completely understood. In this study, we used SGBS adipocytes and a model of human adipose tissue inflammation to examine the effects of resveratrol on the production of PAI-1. Treatment of SGBS adipocytes with resveratrol reduced PAI-1 mRNA and protein in a time- and concentration-dependent manner. Further experiments showed that obesity-associated inflammatory conditions lead to the upregulation of PAI-1 gene expression which was antagonized by resveratrol. Although signaling via PI3K, Sirt1, AMPK, ROS, and Nrf2 appeared to play a significant role in the modulation of PAI-1 gene expression under noninflammatory conditions, those signaling components were not involved in mediating the resveratrol effects on PAI-1 production under inflammatory conditions. Instead, we demonstrate that the resveratrol effects on PAI-1 induction under inflammatory conditions were mediated via inhibition of the NFκB pathway. Together, resveratrol can act as NFκB inhibitor in adipocytes and thus the subsequently reduced PAI-1 expression in inflamed adipose tissue might provide a new insight towards novel treatment options of obesity.http://dx.doi.org/10.1155/2013/793525 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ivana Zagotta Elitsa Y. Dimova Jan-Bernd Funcke Martin Wabitsch Thomas Kietzmann Pamela Fischer-Posovszky |
spellingShingle |
Ivana Zagotta Elitsa Y. Dimova Jan-Bernd Funcke Martin Wabitsch Thomas Kietzmann Pamela Fischer-Posovszky Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue Oxidative Medicine and Cellular Longevity |
author_facet |
Ivana Zagotta Elitsa Y. Dimova Jan-Bernd Funcke Martin Wabitsch Thomas Kietzmann Pamela Fischer-Posovszky |
author_sort |
Ivana Zagotta |
title |
Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue |
title_short |
Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue |
title_full |
Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue |
title_fullStr |
Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue |
title_full_unstemmed |
Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue |
title_sort |
resveratrol suppresses pai-1 gene expression in a human in vitro model of inflamed adipose tissue |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2013-01-01 |
description |
Increased plasminogen activator inhibitor-1 (PAI-1) levels are associated with a number of pathophysiological complications; among them is obesity. Resveratrol was proposed to improve obesity-related health problems, but the effect of resveratrol on PAI-1 gene expression in obesity is not completely understood. In this study, we used SGBS adipocytes and a model of human adipose tissue inflammation to examine the effects of resveratrol on the production of PAI-1. Treatment of SGBS adipocytes with resveratrol reduced PAI-1 mRNA and protein in a time- and concentration-dependent manner. Further experiments showed that obesity-associated inflammatory conditions lead to the upregulation of PAI-1 gene expression which was antagonized by resveratrol. Although signaling via PI3K, Sirt1, AMPK, ROS, and Nrf2 appeared to play a significant role in the modulation of PAI-1 gene expression under noninflammatory conditions, those signaling components were not involved in mediating the resveratrol effects on PAI-1 production under inflammatory conditions. Instead, we demonstrate that the resveratrol effects on PAI-1 induction under inflammatory conditions were mediated via inhibition of the NFκB pathway. Together, resveratrol can act as NFκB inhibitor in adipocytes and thus the subsequently reduced PAI-1 expression in inflamed adipose tissue might provide a new insight towards novel treatment options of obesity. |
url |
http://dx.doi.org/10.1155/2013/793525 |
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