Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers
Genetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the phenotypic level using morphological characters. The results indicated that there were considerable variations among the different studied cultivars. Culti...
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Sultan Qaboos University
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doaj-23b7abf478e643b3871b67b9ebba1dee2020-11-25T03:25:48ZengSultan Qaboos UniversityJournal of Agricultural and Marine Sciences2410-10602410-10792007-01-01120354210.24200/jams.vol12iss0pp35-42631Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD MarkersAbd-Rahman Mohamed M. Al-Moshileh0Mohamed Zaky El-Shinawy1Mohamed Ibrahim Motawei2Department of Plant Production and Protection, College of Agriculture and Veterinary Medicine, Al-Qassim University, Saudi ArabiaHorticulture Department, Faculty of Agriculture, Ain Shams University, Shobra El-Khima, Cairo, EgyptDepartment of Plant Production and Protection, College of Agriculture and Veterinary Medicine, Al-Qassim University, Saudi ArabiaGenetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the phenotypic level using morphological characters. The results indicated that there were considerable variations among the different studied cultivars. Cultivars Victoria, Frizia and Safaren had the highest chlorophyll content compared to the other cultivars in both seasons. The largest leaf area and leaf dry weight were measured in cultivar Safaren and Mondial in both seasons. Cultivars Aboulx and Mondia produced the highest tuber yield in both seasons. On the other hand, cultivars Victoria and Edward were characterized by their considerably lower yield than other studied cultivars. Specific gravity varied considerably among the different potato cultivars. Thirteen random decamer primers were used to amplify DNA via the polymerase chain reaction and 75 RAPDs were generated. The RAPD profiles obtained were successfully used to differentiate potato cultivars. Based on the pair-wise comparison of amplification products, genetic similarity was estimated. The genetic similarity among all potato cultivars ranged from 50 to 92 %. Cultivars Victoria and Etfadoal presented the least similarity (0.50) while cultivars Mondial and Citrix had the greatest similarity (0.92). Etfadoal cultivar displayed the greatest genetic diversity of all cultivars. A dendrogram was constructed using UPGMA analysis. On the basis of this analysis, the cultivars were grouped into three clusters. The polymorphism detected suggests that RAPD markers are reliable for identification of potato cultivars and could be exploited in genetic mapping of populations to tag economically important traits.https://journals.squ.edu.om/index.php/jams/article/view/647potato cultivars, fingerprint, genetic diversity, rapd markers, morphological characters |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abd-Rahman Mohamed M. Al-Moshileh Mohamed Zaky El-Shinawy Mohamed Ibrahim Motawei |
spellingShingle |
Abd-Rahman Mohamed M. Al-Moshileh Mohamed Zaky El-Shinawy Mohamed Ibrahim Motawei Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers Journal of Agricultural and Marine Sciences potato cultivars, fingerprint, genetic diversity, rapd markers, morphological characters |
author_facet |
Abd-Rahman Mohamed M. Al-Moshileh Mohamed Zaky El-Shinawy Mohamed Ibrahim Motawei |
author_sort |
Abd-Rahman Mohamed M. Al-Moshileh |
title |
Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers |
title_short |
Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers |
title_full |
Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers |
title_fullStr |
Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers |
title_full_unstemmed |
Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers |
title_sort |
evaluation of potato cultivars grown in saudi arabia by morphological characters and rapd markers |
publisher |
Sultan Qaboos University |
series |
Journal of Agricultural and Marine Sciences |
issn |
2410-1060 2410-1079 |
publishDate |
2007-01-01 |
description |
Genetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the phenotypic level using morphological characters. The results indicated that there were considerable variations among the different studied cultivars. Cultivars Victoria, Frizia and Safaren had the highest chlorophyll content compared to the other cultivars in both seasons. The largest leaf area and leaf dry weight were measured in cultivar Safaren and Mondial in both seasons. Cultivars Aboulx and Mondia produced the highest tuber yield in both seasons. On the other hand, cultivars Victoria and Edward were characterized by their considerably lower yield than other studied cultivars. Specific gravity varied considerably among the different potato cultivars. Thirteen random decamer primers were used to amplify DNA via the polymerase chain reaction and 75 RAPDs were generated. The RAPD profiles obtained were successfully used to differentiate potato cultivars. Based on the pair-wise comparison of amplification products, genetic similarity was estimated. The genetic similarity among all potato cultivars ranged from 50 to 92 %. Cultivars Victoria and Etfadoal presented the least similarity (0.50) while cultivars Mondial and Citrix had the greatest similarity (0.92). Etfadoal cultivar displayed the greatest genetic diversity of all cultivars. A dendrogram was constructed using UPGMA analysis. On the basis of this analysis, the cultivars were grouped into three clusters. The polymorphism detected suggests that RAPD markers are reliable for identification of potato cultivars and could be exploited in genetic mapping of populations to tag economically important traits. |
topic |
potato cultivars, fingerprint, genetic diversity, rapd markers, morphological characters |
url |
https://journals.squ.edu.om/index.php/jams/article/view/647 |
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