The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells
When silk fibroin particles are used for controlled drug delivery, particle size plays a key role in the location of the carrier on the cells as well as the transport pathway, utilization efficiency, and therapeutic effect of the drugs. In this study, the interactions of different-sized silk fibroin...
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doaj-23bab95f22824432be2a8b9f1def13582020-11-25T03:48:30ZengMDPI AGMaterials1996-19442020-07-01133372337210.3390/ma13153372The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with CellsLongxing Niu0Meijing Shi1Yanfei Feng2Xiaoxiao Sun3Ying Wang4Zhiling Cheng5Mingzhong Li6National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren’ai Road, Industrial Park, Suzhou 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren’ai Road, Industrial Park, Suzhou 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren’ai Road, Industrial Park, Suzhou 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren’ai Road, Industrial Park, Suzhou 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren’ai Road, Industrial Park, Suzhou 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren’ai Road, Industrial Park, Suzhou 215123, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren’ai Road, Industrial Park, Suzhou 215123, ChinaWhen silk fibroin particles are used for controlled drug delivery, particle size plays a key role in the location of the carrier on the cells as well as the transport pathway, utilization efficiency, and therapeutic effect of the drugs. In this study, the interactions of different-sized silk fibroin particles and cell lines were investigated. Silk fibroin microparticles with dry size of 1.9 ± 0.4 μm (2.7 ± 0.3 μm in wet state) and silk fibroin nanoparticles with dry size of 51.5 ± 11.0 nm (174.8 ± 12.5 nm in wet state) were prepared by salting-out method and high-voltage electrospray method, respectively. CdSe/ZnS quantum dots were coupled to the surface of the micro/nanoparticles. Photostability observations indicated that the fluorescence stability of the quantum dots was much higher than that of fluorescein isothiocyanate. In vitro, microparticles and nanoparticles were co-cultured with human umbilical vein endothelial cells EA.hy 926 and cervical cancer cells HeLa, respectively. The fluorescence test and cell viability showed that the EA.hy926 cells tended to be adhered to the microparticle surfaces and the cell proliferation was significantly promoted, while the nanoparticles were more likely to be internalized in HeLa cells and the cell proliferation was notably inhibited. Our findings might provide useful information concerning effective drug delivery that microparticles may be preferred if the drugs need to be delivered to normal cell surface, while nanoparticles may be preferred if the drugs need to be transmitted in tumor cells.https://www.mdpi.com/1996-1944/13/15/3372silk fibroinmicro/nanoparticlesquantum dotsadhesioninternalization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Longxing Niu Meijing Shi Yanfei Feng Xiaoxiao Sun Ying Wang Zhiling Cheng Mingzhong Li |
spellingShingle |
Longxing Niu Meijing Shi Yanfei Feng Xiaoxiao Sun Ying Wang Zhiling Cheng Mingzhong Li The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells Materials silk fibroin micro/nanoparticles quantum dots adhesion internalization |
author_facet |
Longxing Niu Meijing Shi Yanfei Feng Xiaoxiao Sun Ying Wang Zhiling Cheng Mingzhong Li |
author_sort |
Longxing Niu |
title |
The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells |
title_short |
The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells |
title_full |
The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells |
title_fullStr |
The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells |
title_full_unstemmed |
The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells |
title_sort |
interactions of quantum dot-labeled silk fibroin micro/nanoparticles with cells |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2020-07-01 |
description |
When silk fibroin particles are used for controlled drug delivery, particle size plays a key role in the location of the carrier on the cells as well as the transport pathway, utilization efficiency, and therapeutic effect of the drugs. In this study, the interactions of different-sized silk fibroin particles and cell lines were investigated. Silk fibroin microparticles with dry size of 1.9 ± 0.4 μm (2.7 ± 0.3 μm in wet state) and silk fibroin nanoparticles with dry size of 51.5 ± 11.0 nm (174.8 ± 12.5 nm in wet state) were prepared by salting-out method and high-voltage electrospray method, respectively. CdSe/ZnS quantum dots were coupled to the surface of the micro/nanoparticles. Photostability observations indicated that the fluorescence stability of the quantum dots was much higher than that of fluorescein isothiocyanate. In vitro, microparticles and nanoparticles were co-cultured with human umbilical vein endothelial cells EA.hy 926 and cervical cancer cells HeLa, respectively. The fluorescence test and cell viability showed that the EA.hy926 cells tended to be adhered to the microparticle surfaces and the cell proliferation was significantly promoted, while the nanoparticles were more likely to be internalized in HeLa cells and the cell proliferation was notably inhibited. Our findings might provide useful information concerning effective drug delivery that microparticles may be preferred if the drugs need to be delivered to normal cell surface, while nanoparticles may be preferred if the drugs need to be transmitted in tumor cells. |
topic |
silk fibroin micro/nanoparticles quantum dots adhesion internalization |
url |
https://www.mdpi.com/1996-1944/13/15/3372 |
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