RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> Test for Rapid Detection of Polymyxin Resistance in <i>Acinetobacter baumannii</i>
A homemade and culture-based test, relying on the visual detection of the reduction of the resazurin reagent (a cell viability indicator), has been developed for the rapid detection of polymyxin resistance in <i>Acinetobacter baumannii</i>. Here, we evaluated the industrial version of th...
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doaj-23e048cb5e3346f0bc91ef466e4dc8ea2021-05-31T23:39:51ZengMDPI AGAntibiotics2079-63822021-05-011055855810.3390/antibiotics10050558RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> Test for Rapid Detection of Polymyxin Resistance in <i>Acinetobacter baumannii</i>Maxime Bouvier0Mustafa Sadek1Stefano Pomponio2Fernando D’Emidio3Laurent Poirel4Patrice Nordmann5Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandMedical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandLiofilchem, 64026 Roseto degli Abruzzi, ItalyLiofilchem, 64026 Roseto degli Abruzzi, ItalyMedical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandMedical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandA homemade and culture-based test, relying on the visual detection of the reduction of the resazurin reagent (a cell viability indicator), has been developed for the rapid detection of polymyxin resistance in <i>Acinetobacter baumannii</i>. Here, we evaluated the industrial version of this test, the RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> test (Liofilchem, Italy). A well-characterized panel of 68 clinical <i>A. baumannii</i> strains (36 polymyxin-susceptible, 26 polymyxin-resistant <i>A. baumannii</i>, and 6 colistin-heteroresistant isolates) of worldwide origin was tested. All the colistin-susceptible <i>A. baumannii</i> isolates gave negative results according to the RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> test, except for a single isolate that gave a false-positive result. Out of the 26 colistin-resistant <i>A. baumannii</i> strains, 25 were correctly identified as colistin resistant using the RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> test. Only a single colistin-resistant <i>A. baumannii</i> strain gave a false-negative result. Additionally, the six colistin-heteroresistant isolates tested gave positive results. Altogether, the sensitivity and the specificity of the test were found to be 96% and 97%, respectively. The turn-around-time of this easy-to-perform test was 3-4h, which showed excellent reliability for identification of polymyxin resistance in <i>A. baumannii</i>. The RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> test allows a rapid differentiation between polymyxin-susceptible and -resistant <i>A. baumannii</i> isolates, which may contribute to optimization of the use of polymyxins for treating infections due to multidrug-resistant <i>A. baumannii.</i>https://www.mdpi.com/2079-6382/10/5/558colistin<i>Acinetobacter</i>susceptibility testingdiagnostic test |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maxime Bouvier Mustafa Sadek Stefano Pomponio Fernando D’Emidio Laurent Poirel Patrice Nordmann |
spellingShingle |
Maxime Bouvier Mustafa Sadek Stefano Pomponio Fernando D’Emidio Laurent Poirel Patrice Nordmann RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> Test for Rapid Detection of Polymyxin Resistance in <i>Acinetobacter baumannii</i> Antibiotics colistin <i>Acinetobacter</i> susceptibility testing diagnostic test |
author_facet |
Maxime Bouvier Mustafa Sadek Stefano Pomponio Fernando D’Emidio Laurent Poirel Patrice Nordmann |
author_sort |
Maxime Bouvier |
title |
RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> Test for Rapid Detection of Polymyxin Resistance in <i>Acinetobacter baumannii</i> |
title_short |
RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> Test for Rapid Detection of Polymyxin Resistance in <i>Acinetobacter baumannii</i> |
title_full |
RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> Test for Rapid Detection of Polymyxin Resistance in <i>Acinetobacter baumannii</i> |
title_fullStr |
RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> Test for Rapid Detection of Polymyxin Resistance in <i>Acinetobacter baumannii</i> |
title_full_unstemmed |
RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> Test for Rapid Detection of Polymyxin Resistance in <i>Acinetobacter baumannii</i> |
title_sort |
rapidresa polymyxin <i>acinetobacter</i> np<sup>®</sup> test for rapid detection of polymyxin resistance in <i>acinetobacter baumannii</i> |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2021-05-01 |
description |
A homemade and culture-based test, relying on the visual detection of the reduction of the resazurin reagent (a cell viability indicator), has been developed for the rapid detection of polymyxin resistance in <i>Acinetobacter baumannii</i>. Here, we evaluated the industrial version of this test, the RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> test (Liofilchem, Italy). A well-characterized panel of 68 clinical <i>A. baumannii</i> strains (36 polymyxin-susceptible, 26 polymyxin-resistant <i>A. baumannii</i>, and 6 colistin-heteroresistant isolates) of worldwide origin was tested. All the colistin-susceptible <i>A. baumannii</i> isolates gave negative results according to the RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> test, except for a single isolate that gave a false-positive result. Out of the 26 colistin-resistant <i>A. baumannii</i> strains, 25 were correctly identified as colistin resistant using the RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> test. Only a single colistin-resistant <i>A. baumannii</i> strain gave a false-negative result. Additionally, the six colistin-heteroresistant isolates tested gave positive results. Altogether, the sensitivity and the specificity of the test were found to be 96% and 97%, respectively. The turn-around-time of this easy-to-perform test was 3-4h, which showed excellent reliability for identification of polymyxin resistance in <i>A. baumannii</i>. The RapidResa Polymyxin <i>Acinetobacter</i> NP<sup>®</sup> test allows a rapid differentiation between polymyxin-susceptible and -resistant <i>A. baumannii</i> isolates, which may contribute to optimization of the use of polymyxins for treating infections due to multidrug-resistant <i>A. baumannii.</i> |
topic |
colistin <i>Acinetobacter</i> susceptibility testing diagnostic test |
url |
https://www.mdpi.com/2079-6382/10/5/558 |
work_keys_str_mv |
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