Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri
Abstract Background Arcobacter faecis and A. lanthieri are two newly classified species of genus Arcobacter. The prevalence and distribution of virulence, antibiotic resistance and toxin (VAT) genes in these species are required to assess their potential pathogenic health impacts to humans and anima...
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doaj-23e860729e0442cea8cb4697b0f6c8b32020-11-25T02:44:20ZengBMCBMC Microbiology1471-21802019-01-0119111510.1186/s12866-018-1357-7Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieriMatthew Zambri0Michel Cloutier1Zaky Adam2David R. Lapen3Graham Wilkes4Mark Sunohara5Edward Topp6Guylaine Talbot7Izhar U. H. Khan8Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food CanadaOttawa Research and Development Centre (ORDC), Agriculture and Agri-Food CanadaOttawa Research and Development Centre (ORDC), Agriculture and Agri-Food CanadaOttawa Research and Development Centre (ORDC), Agriculture and Agri-Food CanadaOttawa Research and Development Centre (ORDC), Agriculture and Agri-Food CanadaOttawa Research and Development Centre (ORDC), Agriculture and Agri-Food CanadaLondon Research and Development Centre (LRDC), Agriculture and Agri-Food CanadaSherbrooke Research and Development Centre (SRDC), Agriculture and Agri-Food Canada, SherbrookeOttawa Research and Development Centre (ORDC), Agriculture and Agri-Food CanadaAbstract Background Arcobacter faecis and A. lanthieri are two newly classified species of genus Arcobacter. The prevalence and distribution of virulence, antibiotic resistance and toxin (VAT) genes in these species are required to assess their potential pathogenic health impacts to humans and animals. This study (i) developed species- and gene-specific primer pairs for the detection of six virulence, two antibiotic resistance, and three toxin genes in two target species; (ii) optimized eight single-tube multiplex and three monoplex PCR protocols using the newly developed species- and gene-specific primers; and (iii) conducted specificity and sensitivity evaluations as well as validation of eleven mono- and multiplex PCR assays by testing A. faecis (n= 29) and A. lanthieri (n= 10) strains isolated from various fecal and agricultural water sources to determine the prevalence and distribution of VAT genes and assess the degree of pathogenicity within the two species. Results Detection of all ten and eleven target VAT genes, and expression of cytolethal distending toxin (cdtA, cdtB and cdtC) genes in A. faecis and A. lanthieri reference strains with high frequency in field isolates suggest that they are potentially pathogenic strains. These findings indicate that these two species can pose a health risk to humans and animals. Conclusions The study results show that the developed mono- and multiplex PCR (mPCR) assays are simple, rapid, reliable and sensitive for the simultaneous assessment of the potential pathogenicity and antibiotic resistance profiling of tet(O) and tet(W) genes in these two newly discovered species. Also, these assays can be useful in diagnostic and analytical laboratories to determine the pathotypes and assessment of the virulence and toxin factors associated to human and animal infections.http://link.springer.com/article/10.1186/s12866-018-1357-7Mono- and multiplex PCRArcobacter faecisA. lanthieriPathogenicityVirulenceAntibiotic resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matthew Zambri Michel Cloutier Zaky Adam David R. Lapen Graham Wilkes Mark Sunohara Edward Topp Guylaine Talbot Izhar U. H. Khan |
spellingShingle |
Matthew Zambri Michel Cloutier Zaky Adam David R. Lapen Graham Wilkes Mark Sunohara Edward Topp Guylaine Talbot Izhar U. H. Khan Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri BMC Microbiology Mono- and multiplex PCR Arcobacter faecis A. lanthieri Pathogenicity Virulence Antibiotic resistance |
author_facet |
Matthew Zambri Michel Cloutier Zaky Adam David R. Lapen Graham Wilkes Mark Sunohara Edward Topp Guylaine Talbot Izhar U. H. Khan |
author_sort |
Matthew Zambri |
title |
Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri |
title_short |
Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri |
title_full |
Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri |
title_fullStr |
Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri |
title_full_unstemmed |
Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri |
title_sort |
novel virulence, antibiotic resistance and toxin gene-specific pcr-based assays for rapid pathogenicity assessment of arcobacter faecis and arcobacter lanthieri |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2019-01-01 |
description |
Abstract Background Arcobacter faecis and A. lanthieri are two newly classified species of genus Arcobacter. The prevalence and distribution of virulence, antibiotic resistance and toxin (VAT) genes in these species are required to assess their potential pathogenic health impacts to humans and animals. This study (i) developed species- and gene-specific primer pairs for the detection of six virulence, two antibiotic resistance, and three toxin genes in two target species; (ii) optimized eight single-tube multiplex and three monoplex PCR protocols using the newly developed species- and gene-specific primers; and (iii) conducted specificity and sensitivity evaluations as well as validation of eleven mono- and multiplex PCR assays by testing A. faecis (n= 29) and A. lanthieri (n= 10) strains isolated from various fecal and agricultural water sources to determine the prevalence and distribution of VAT genes and assess the degree of pathogenicity within the two species. Results Detection of all ten and eleven target VAT genes, and expression of cytolethal distending toxin (cdtA, cdtB and cdtC) genes in A. faecis and A. lanthieri reference strains with high frequency in field isolates suggest that they are potentially pathogenic strains. These findings indicate that these two species can pose a health risk to humans and animals. Conclusions The study results show that the developed mono- and multiplex PCR (mPCR) assays are simple, rapid, reliable and sensitive for the simultaneous assessment of the potential pathogenicity and antibiotic resistance profiling of tet(O) and tet(W) genes in these two newly discovered species. Also, these assays can be useful in diagnostic and analytical laboratories to determine the pathotypes and assessment of the virulence and toxin factors associated to human and animal infections. |
topic |
Mono- and multiplex PCR Arcobacter faecis A. lanthieri Pathogenicity Virulence Antibiotic resistance |
url |
http://link.springer.com/article/10.1186/s12866-018-1357-7 |
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