Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin
Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by...
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doaj-23f6472d20e14be49fe6eb0648f587332020-11-25T00:46:29ZengMDPI AGMolecules1420-30492014-01-011911258127210.3390/molecules19011258molecules19011258Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, EmodinChing-Yi Yiu0Shih-Ying Chen1Tsai-Hsiu Yang2Che-Jung Chang3Dong-Bor Yeh4Yi-Jie Chen5Tsuey-Pin Lin6Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, TaiwanDepartment of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, TaiwanDepartment of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, TaiwanDepartment of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan 717, TaiwanDepartment of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan 717, TaiwanDepartment of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, TaiwanDepartment of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, TaiwanPolygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.http://www.mdpi.com/1420-3049/19/1/1258Polygonum cuspidatumemodinantiviral activityEBV |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ching-Yi Yiu Shih-Ying Chen Tsai-Hsiu Yang Che-Jung Chang Dong-Bor Yeh Yi-Jie Chen Tsuey-Pin Lin |
spellingShingle |
Ching-Yi Yiu Shih-Ying Chen Tsai-Hsiu Yang Che-Jung Chang Dong-Bor Yeh Yi-Jie Chen Tsuey-Pin Lin Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin Molecules Polygonum cuspidatum emodin antiviral activity EBV |
author_facet |
Ching-Yi Yiu Shih-Ying Chen Tsai-Hsiu Yang Che-Jung Chang Dong-Bor Yeh Yi-Jie Chen Tsuey-Pin Lin |
author_sort |
Ching-Yi Yiu |
title |
Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin |
title_short |
Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin |
title_full |
Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin |
title_fullStr |
Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin |
title_full_unstemmed |
Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin |
title_sort |
inhibition of epstein-barr virus lytic cycle by an ethyl acetate subfraction separated from polygonum cuspidatum root and its major component, emodin |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2014-01-01 |
description |
Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug. |
topic |
Polygonum cuspidatum emodin antiviral activity EBV |
url |
http://www.mdpi.com/1420-3049/19/1/1258 |
work_keys_str_mv |
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