The Significance of TROP2 Expression in Predicting Mutations in Papillary Thyroid Carcinoma
Background Trophoblast antigen 2 (TROP2) is a human trophoblast cell-surface glycoprotein that is overexpressed in several types of epithelial cancers, and is suggested to be associated with an unfavorable prognosis. BRAF mutations are the most common genetic alteration in papillary thyroid carcinom...
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doaj-23fc090db46e4f44ae46a727bb804a432020-11-24T23:58:50ZengKorean Society of Pathologists & the Korean Society for CytopathologyJournal of Pathology and Translational Medicine2383-78372383-78452018-01-01521142010.4132/jptm.2017.10.1716740The Significance of TROP2 Expression in Predicting Mutations in Papillary Thyroid CarcinomaJoon Seog Kong0Hyeon Jin Kim1Min-Jung Kim2Areumnuri Kim3Dalnim Lee4Kanghee Han5Sunhoo Park6Jae Soo Koh7Jae Kyung Myung8 Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea Laboratory of Radiation Exposure and Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea Laboratory of Radiation Exposure and Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea Laboratory of Radiation Exposure and Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea Laboratory of Radiation Exposure and Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea Department of Pathology, Korea Cancer Center Hospital, Seoul, KoreaBackground Trophoblast antigen 2 (TROP2) is a human trophoblast cell-surface glycoprotein that is overexpressed in several types of epithelial cancers, and is suggested to be associated with an unfavorable prognosis. BRAF mutations are the most common genetic alteration in papillary thyroid carcinoma (PTC). We evaluated the correlation between TROP2 expression and BRAF mutation in PTC. Methods First, we carried out pyrosequencing for BRAF mutations and immunohistochemistry for TROP2 expression with a tissue microarray consisting of 52 PTC cases. Membranous staining in at least 5% of tumor cells was designated as positive staining and we analyzed the relationship between TROP2 expression and diverse clinicopathological factors, including BRAF mutation. Second, we tested TROP2 mRNA expression in three thyroid cancer cell lines with BRAF mutations (BCPAP, SNU790, and 8505C) and a normal thyroid cell line. Additionally, we checked TROP2 protein levels in a normal thyroid cell line after introduction of the BRAF V600E mutation. Results In this study, 21 of 26 cases with BRAF mutation showed TROP2 immunoreactivity, whereas all 26 cases without BRAF mutation showed no immunoreactivity for TROP2 with a statistically significant difference (p<.001). Upregulation of TROP2 mRNA was observed in all three thyroid cancer cell lines, but not in the normal thyroid cell line. Interestingly, however, the TROP2 expression was increased in the normal thyroid cell line after introduction of the BRAF V600E mutation. Conclusions Based on these results, we concluded that TROP2 expression is significantly associated with BRAF mutation and that TROP2 immunohistochemistry could be used for predicting BRAF mutations or diagnosing papillary thyroid carcinoma.http://www.jpatholtm.org/upload/pdf/jptm-2017-10-17.pdfTROP2 mutationPapillary thyroid carcinoma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joon Seog Kong Hyeon Jin Kim Min-Jung Kim Areumnuri Kim Dalnim Lee Kanghee Han Sunhoo Park Jae Soo Koh Jae Kyung Myung |
spellingShingle |
Joon Seog Kong Hyeon Jin Kim Min-Jung Kim Areumnuri Kim Dalnim Lee Kanghee Han Sunhoo Park Jae Soo Koh Jae Kyung Myung The Significance of TROP2 Expression in Predicting Mutations in Papillary Thyroid Carcinoma Journal of Pathology and Translational Medicine TROP2 mutation Papillary thyroid carcinoma |
author_facet |
Joon Seog Kong Hyeon Jin Kim Min-Jung Kim Areumnuri Kim Dalnim Lee Kanghee Han Sunhoo Park Jae Soo Koh Jae Kyung Myung |
author_sort |
Joon Seog Kong |
title |
The Significance of TROP2 Expression in Predicting Mutations in Papillary Thyroid Carcinoma |
title_short |
The Significance of TROP2 Expression in Predicting Mutations in Papillary Thyroid Carcinoma |
title_full |
The Significance of TROP2 Expression in Predicting Mutations in Papillary Thyroid Carcinoma |
title_fullStr |
The Significance of TROP2 Expression in Predicting Mutations in Papillary Thyroid Carcinoma |
title_full_unstemmed |
The Significance of TROP2 Expression in Predicting Mutations in Papillary Thyroid Carcinoma |
title_sort |
significance of trop2 expression in predicting mutations in papillary thyroid carcinoma |
publisher |
Korean Society of Pathologists & the Korean Society for Cytopathology |
series |
Journal of Pathology and Translational Medicine |
issn |
2383-7837 2383-7845 |
publishDate |
2018-01-01 |
description |
Background Trophoblast antigen 2 (TROP2) is a human trophoblast cell-surface glycoprotein that is overexpressed in several types of epithelial cancers, and is suggested to be associated with an unfavorable prognosis. BRAF mutations are the most common genetic alteration in papillary thyroid carcinoma (PTC). We evaluated the correlation between TROP2 expression and BRAF mutation in PTC. Methods First, we carried out pyrosequencing for BRAF mutations and immunohistochemistry for TROP2 expression with a tissue microarray consisting of 52 PTC cases. Membranous staining in at least 5% of tumor cells was designated as positive staining and we analyzed the relationship between TROP2 expression and diverse clinicopathological factors, including BRAF mutation. Second, we tested TROP2 mRNA expression in three thyroid cancer cell lines with BRAF mutations (BCPAP, SNU790, and 8505C) and a normal thyroid cell line. Additionally, we checked TROP2 protein levels in a normal thyroid cell line after introduction of the BRAF V600E mutation. Results In this study, 21 of 26 cases with BRAF mutation showed TROP2 immunoreactivity, whereas all 26 cases without BRAF mutation showed no immunoreactivity for TROP2 with a statistically significant difference (p<.001). Upregulation of TROP2 mRNA was observed in all three thyroid cancer cell lines, but not in the normal thyroid cell line. Interestingly, however, the TROP2 expression was increased in the normal thyroid cell line after introduction of the BRAF V600E mutation. Conclusions Based on these results, we concluded that TROP2 expression is significantly associated with BRAF mutation and that TROP2 immunohistochemistry could be used for predicting BRAF mutations or diagnosing papillary thyroid carcinoma. |
topic |
TROP2 mutation Papillary thyroid carcinoma |
url |
http://www.jpatholtm.org/upload/pdf/jptm-2017-10-17.pdf |
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