Desenvolvimento e validação de método analítico para quantificação do fármaco bevacizumabe por cromatografia a líquido de alta eficiência

Desenvolvimento e validação de método analítico para quantificação do fármaco bevacizumabe por cromatografia a líquido de alta eficiência

In this study, an analytical method was developed and validated for quantitation of the drug bevacizumab (Avastin®) by high performance liquid chromatography (HPLC). The HPLC column was a BioSuite 250® HR SEC, 300 x 7.8 mm x 5 µm (Waters, USA). The mobile phase consisted of phosphate buffered saline...

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Bibliographic Details
Main Authors: Elionai Cassiana de Lima Gomes, Armando da Silva Cunha Júnior, Maria Irene Yoshida, Rodrigo Jorge
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2012-01-01
Series:Química Nova
Subjects:
bevacizumab
high performance liquid chromatography
validation
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300029&lng=en&tlng=en
Description
Summary:In this study, an analytical method was developed and validated for quantitation of the drug bevacizumab (Avastin®) by high performance liquid chromatography (HPLC). The HPLC column was a BioSuite 250® HR SEC, 300 x 7.8 mm x 5 µm (Waters, USA). The mobile phase consisted of phosphate buffered saline (PBS). The results revealed that the method was specific, precise, accurate, robust and linear (r² = 0.998) from 5 to 75 µg mL-1. Therefore, this method can be used in drug release studies or in quality control ampoules of the drug.
ISSN:1678-7064

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