Pretreating Luffa Sponge (Luffa cylindrica L.) with Concentrated Phosphoric Acid and Subsequent Enzymatic Saccharification
Luffa was evaluated as a potential energy crop. A considerable amount of luffa sponge biomass can be grown in a vertical direction with approximately 70% polysaccharide content and low lignin content. When concentrated H3PO4 was employed to pretreat luffa sponge, hemicelluloses were the most sensiti...
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North Carolina State University
2015-11-01
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doaj-24367086c2a64fe09f8a3bb30dde92652020-11-24T23:48:57ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-11-0111189991210.15376/biores.11.1.899-912Pretreating Luffa Sponge (Luffa cylindrica L.) with Concentrated Phosphoric Acid and Subsequent Enzymatic SaccharificationQing Wang0Fei Shen1Gang Yang2Yanzong Zhang3Shihuai Deng4Yaodong Hu5Jing Zhang6Chun Song7Yongmei Zeng8 Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China; China Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China; China Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China; China Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China; China Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China; ChinaInstitute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China; China Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China; China Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China; ChinaDepartment of Information Consultation and Integration, Library of Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China; ChinaLuffa was evaluated as a potential energy crop. A considerable amount of luffa sponge biomass can be grown in a vertical direction with approximately 70% polysaccharide content and low lignin content. When concentrated H3PO4 was employed to pretreat luffa sponge, hemicelluloses were the most sensitive component, followed by cellulose and lignin. Hemicellulose solubilization and cellulose loss positively responded to the elevated temperature, time, H3PO4 concentration, and dosage for pretreatment. However, lignin solubilization was not affected greatly. Although the initial hydrolysis rate was accelerated by increasing pretreatment temperature, the final glucose conversion was reduced as temperature was raised higher than 50 °C. Prolonging pretreatment time was positively correlated to enzymatic digestibility. When H3PO4 concentration was lower than 80%, the final glucose conversion was increased with increasing H3PO4 concentration. Increasing H3PO4 concentration to 84% for pretreatment caused the final glucose conversion to decrease slightly, although the hydrolysis rate was initially accelerated. Additionally, an improvement of glucose conversion was obtained with increasing the substrate-to-phosphoric acid ratio. However, the improvement was not cost-effective, as the ratio was lower than 1-to-8.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_899_Wang_Pretreating_Luffa_Sponge_SaccharificationPretreatmentLuffa spongeEnzymatic hydrolysisConcentrated phosphoric acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qing Wang Fei Shen Gang Yang Yanzong Zhang Shihuai Deng Yaodong Hu Jing Zhang Chun Song Yongmei Zeng |
spellingShingle |
Qing Wang Fei Shen Gang Yang Yanzong Zhang Shihuai Deng Yaodong Hu Jing Zhang Chun Song Yongmei Zeng Pretreating Luffa Sponge (Luffa cylindrica L.) with Concentrated Phosphoric Acid and Subsequent Enzymatic Saccharification BioResources Pretreatment Luffa sponge Enzymatic hydrolysis Concentrated phosphoric acid |
author_facet |
Qing Wang Fei Shen Gang Yang Yanzong Zhang Shihuai Deng Yaodong Hu Jing Zhang Chun Song Yongmei Zeng |
author_sort |
Qing Wang |
title |
Pretreating Luffa Sponge (Luffa cylindrica L.) with Concentrated Phosphoric Acid and Subsequent Enzymatic Saccharification |
title_short |
Pretreating Luffa Sponge (Luffa cylindrica L.) with Concentrated Phosphoric Acid and Subsequent Enzymatic Saccharification |
title_full |
Pretreating Luffa Sponge (Luffa cylindrica L.) with Concentrated Phosphoric Acid and Subsequent Enzymatic Saccharification |
title_fullStr |
Pretreating Luffa Sponge (Luffa cylindrica L.) with Concentrated Phosphoric Acid and Subsequent Enzymatic Saccharification |
title_full_unstemmed |
Pretreating Luffa Sponge (Luffa cylindrica L.) with Concentrated Phosphoric Acid and Subsequent Enzymatic Saccharification |
title_sort |
pretreating luffa sponge (luffa cylindrica l.) with concentrated phosphoric acid and subsequent enzymatic saccharification |
publisher |
North Carolina State University |
series |
BioResources |
issn |
1930-2126 1930-2126 |
publishDate |
2015-11-01 |
description |
Luffa was evaluated as a potential energy crop. A considerable amount of luffa sponge biomass can be grown in a vertical direction with approximately 70% polysaccharide content and low lignin content. When concentrated H3PO4 was employed to pretreat luffa sponge, hemicelluloses were the most sensitive component, followed by cellulose and lignin. Hemicellulose solubilization and cellulose loss positively responded to the elevated temperature, time, H3PO4 concentration, and dosage for pretreatment. However, lignin solubilization was not affected greatly. Although the initial hydrolysis rate was accelerated by increasing pretreatment temperature, the final glucose conversion was reduced as temperature was raised higher than 50 °C. Prolonging pretreatment time was positively correlated to enzymatic digestibility. When H3PO4 concentration was lower than 80%, the final glucose conversion was increased with increasing H3PO4 concentration. Increasing H3PO4 concentration to 84% for pretreatment caused the final glucose conversion to decrease slightly, although the hydrolysis rate was initially accelerated. Additionally, an improvement of glucose conversion was obtained with increasing the substrate-to-phosphoric acid ratio. However, the improvement was not cost-effective, as the ratio was lower than 1-to-8. |
topic |
Pretreatment Luffa sponge Enzymatic hydrolysis Concentrated phosphoric acid |
url |
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_899_Wang_Pretreating_Luffa_Sponge_Saccharification |
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