The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys
The brown marmorated stink bug, Halyomorpha halys, is an invasive, phytophagous stink bug of global importance for agriculture. Tissue-specific transcriptomic analysis of the accessory salivary gland, principal salivary gland (PSG) and gut resulted in identification of 234 putative protease and 166...
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doaj-2437b8b9115f487ead203c6557da382b2020-11-25T02:12:17ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-10-011010.3389/fphys.2019.01255492476The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halysSijun Liu0Bryony C. Bonning1Department of Entomology, Iowa State University, Ames, IA, United StatesDepartment of Entomology and Nematology, University of Florida, Gainesville, FL, United StatesThe brown marmorated stink bug, Halyomorpha halys, is an invasive, phytophagous stink bug of global importance for agriculture. Tissue-specific transcriptomic analysis of the accessory salivary gland, principal salivary gland (PSG) and gut resulted in identification of 234 putative protease and 166 putative nuclease sequences. By mapping the previously reported proteomes of H. halys watery saliva (WS) and sheath saliva to protein sequences translated from the assembled transcripts, 22 proteases and two nucleases in the saliva were identified. Of these, 19 proteases and both nucleases were present in the WS. The majority of proteases and nucleases found in WS were derived from the PSG, in line with ultrastructural observations, which suggest active protein synthesis and secretion by this tissue. The highly transcribed digestive proteases and nucleases of H. halys were similar to those of the southern green stink bug, Nezara viridula, indicating that these pentatomid stink bugs utilize a similar suite of proteases and nucleases for digestion of plant material. The comprehensive data set for the H. halys salivary glands and gut generated by this study provides an additional resource for further understanding of the biology of this pestiferous species.https://www.frontiersin.org/article/10.3389/fphys.2019.01255/fullHalyomorpha halysproteasenucleasestink bugsalivary glandgut |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sijun Liu Bryony C. Bonning |
spellingShingle |
Sijun Liu Bryony C. Bonning The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys Frontiers in Physiology Halyomorpha halys protease nuclease stink bug salivary gland gut |
author_facet |
Sijun Liu Bryony C. Bonning |
author_sort |
Sijun Liu |
title |
The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys |
title_short |
The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys |
title_full |
The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys |
title_fullStr |
The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys |
title_full_unstemmed |
The Principal Salivary Gland Is the Primary Source of Digestive Enzymes in the Saliva of the Brown Marmorated Stink Bug, Halyomorpha halys |
title_sort |
principal salivary gland is the primary source of digestive enzymes in the saliva of the brown marmorated stink bug, halyomorpha halys |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2019-10-01 |
description |
The brown marmorated stink bug, Halyomorpha halys, is an invasive, phytophagous stink bug of global importance for agriculture. Tissue-specific transcriptomic analysis of the accessory salivary gland, principal salivary gland (PSG) and gut resulted in identification of 234 putative protease and 166 putative nuclease sequences. By mapping the previously reported proteomes of H. halys watery saliva (WS) and sheath saliva to protein sequences translated from the assembled transcripts, 22 proteases and two nucleases in the saliva were identified. Of these, 19 proteases and both nucleases were present in the WS. The majority of proteases and nucleases found in WS were derived from the PSG, in line with ultrastructural observations, which suggest active protein synthesis and secretion by this tissue. The highly transcribed digestive proteases and nucleases of H. halys were similar to those of the southern green stink bug, Nezara viridula, indicating that these pentatomid stink bugs utilize a similar suite of proteases and nucleases for digestion of plant material. The comprehensive data set for the H. halys salivary glands and gut generated by this study provides an additional resource for further understanding of the biology of this pestiferous species. |
topic |
Halyomorpha halys protease nuclease stink bug salivary gland gut |
url |
https://www.frontiersin.org/article/10.3389/fphys.2019.01255/full |
work_keys_str_mv |
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