Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development
Morphological, biological, and genetic characteristics of a virulent Siphoviridae phage, named vB-EcoS-95, is reported. This phage was isolated from urban sewage. It was found to infect some Escherichia coli strains giving clear plaques. The genome of this phage is composed of 50,910 bp and contains...
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doaj-244e7f8e191949d3ad02788e5e87e5322020-11-25T00:03:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-01-01910.3389/fmicb.2018.03326411311Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic DevelopmentGracja Topka0Sylwia Bloch1Bożena Nejman-Faleńczyk2Tomasz Gąsior3Agata Jurczak-Kurek4Agnieszka Necel5Aleksandra Dydecka6Malwina Richert7Grzegorz Węgrzyn8Alicja Węgrzyn9Department of Molecular BiologyUniversity of Gdańsk, Gdańsk, PolandDepartment of Molecular BiologyUniversity of Gdańsk, Gdańsk, PolandDepartment of Molecular BiologyUniversity of Gdańsk, Gdańsk, PolandLaboratory of Molecular Biology, Institute of Biochemistry and BiophysicsPolish Academy of Sciences, Gdańsk, PolandDepartment of Molecular EvolutionUniversity of Gdańsk, Gdańsk, PolandDepartment of Molecular BiologyUniversity of Gdańsk, Gdańsk, PolandDepartment of Molecular BiologyUniversity of Gdańsk, Gdańsk, PolandLaboratory of Electron MicroscopyUniversity of Gdańsk, Gdańsk, PolandDepartment of Molecular BiologyUniversity of Gdańsk, Gdańsk, PolandLaboratory of Molecular Biology, Institute of Biochemistry and BiophysicsPolish Academy of Sciences, Gdańsk, PolandMorphological, biological, and genetic characteristics of a virulent Siphoviridae phage, named vB-EcoS-95, is reported. This phage was isolated from urban sewage. It was found to infect some Escherichia coli strains giving clear plaques. The genome of this phage is composed of 50,910 bp and contains 89 ORFs. Importantly, none of the predicted ORFs shows any similarity with known pathogenic factors that would prevent its use in medicine. Genome sequence analysis of vB-EcoS-95 revealed 74% similarity to genomic sequence of Shigella phage pSf-1. Compared to pSf-1, phage vb-EcoS-95 does not infect Shigella strains and has an efficient bacteriolytic activity against some E. coli strains. One-step growth analysis revealed that this phage has a very short latent period (4 min), and average burst size of 115 plaque forming units per cell, which points to its high infectivity of host cells and strong lytic activity. The bacteriolytic effect of vB-EcoS-95 was tested also on biofilm-producing strains. These results indicate that vB-EcoS-95 is a newly discovered E. coli phage that may be potentially used to control the formation of biofilms.https://www.frontiersin.org/article/10.3389/fmicb.2018.03326/fullbacteriophagecoliphagelytic developmentgenomic analysisbiofilm |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gracja Topka Sylwia Bloch Bożena Nejman-Faleńczyk Tomasz Gąsior Agata Jurczak-Kurek Agnieszka Necel Aleksandra Dydecka Malwina Richert Grzegorz Węgrzyn Alicja Węgrzyn |
spellingShingle |
Gracja Topka Sylwia Bloch Bożena Nejman-Faleńczyk Tomasz Gąsior Agata Jurczak-Kurek Agnieszka Necel Aleksandra Dydecka Malwina Richert Grzegorz Węgrzyn Alicja Węgrzyn Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development Frontiers in Microbiology bacteriophage coliphage lytic development genomic analysis biofilm |
author_facet |
Gracja Topka Sylwia Bloch Bożena Nejman-Faleńczyk Tomasz Gąsior Agata Jurczak-Kurek Agnieszka Necel Aleksandra Dydecka Malwina Richert Grzegorz Węgrzyn Alicja Węgrzyn |
author_sort |
Gracja Topka |
title |
Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development |
title_short |
Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development |
title_full |
Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development |
title_fullStr |
Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development |
title_full_unstemmed |
Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development |
title_sort |
characterization of bacteriophage vb-ecos-95, isolated from urban sewage and revealing extremely rapid lytic development |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-01-01 |
description |
Morphological, biological, and genetic characteristics of a virulent Siphoviridae phage, named vB-EcoS-95, is reported. This phage was isolated from urban sewage. It was found to infect some Escherichia coli strains giving clear plaques. The genome of this phage is composed of 50,910 bp and contains 89 ORFs. Importantly, none of the predicted ORFs shows any similarity with known pathogenic factors that would prevent its use in medicine. Genome sequence analysis of vB-EcoS-95 revealed 74% similarity to genomic sequence of Shigella phage pSf-1. Compared to pSf-1, phage vb-EcoS-95 does not infect Shigella strains and has an efficient bacteriolytic activity against some E. coli strains. One-step growth analysis revealed that this phage has a very short latent period (4 min), and average burst size of 115 plaque forming units per cell, which points to its high infectivity of host cells and strong lytic activity. The bacteriolytic effect of vB-EcoS-95 was tested also on biofilm-producing strains. These results indicate that vB-EcoS-95 is a newly discovered E. coli phage that may be potentially used to control the formation of biofilms. |
topic |
bacteriophage coliphage lytic development genomic analysis biofilm |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.03326/full |
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