hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated
RNA binding proteins (RBPs) play a key role in cellular growth, homoeostasis and survival and are tightly regulated. A deep understanding of their spatiotemporal regulation is needed to understand their contribution to physiology and pathology. Here, we have characterized the spatiotemporal expressi...
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doaj-2457c80e01774b2b8bc6697879d5b7c22021-09-24T07:02:10ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2021-09-011510.3389/fnins.2021.724307724307hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally RegulatedMyriam Gagné0Myriam Gagné1Jade-Emmanuelle Deshaies2Hadjara Sidibé3Hadjara Sidibé4Yousri Benchaar5Danielle Arbour6Alicia Dubinski7Alicia Dubinski8Gurleen Litt9Sarah Peyrard10Richard Robitaille11Chantelle F. Sephton12Christine Vande Velde13Christine Vande Velde14Department of Biochemistry, Université de Montréal, Montréal, QC, CanadaCentre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, CanadaCentre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, CanadaCentre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartment of Neurosciences, Université de Montréal, Montréal, QC, CanadaDepartment of Psychiatry and Neuroscience, CERVO Brain Research Centre, Laval University, Quebec City, QC, CanadaDepartment of Neurosciences, Université de Montréal, Montréal, QC, CanadaCentre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartment of Neurosciences, Université de Montréal, Montréal, QC, CanadaCentre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, CanadaCentre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartment of Neurosciences, Université de Montréal, Montréal, QC, CanadaDepartment of Psychiatry and Neuroscience, CERVO Brain Research Centre, Laval University, Quebec City, QC, CanadaCentre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, CanadaDepartment of Neurosciences, Université de Montréal, Montréal, QC, CanadaRNA binding proteins (RBPs) play a key role in cellular growth, homoeostasis and survival and are tightly regulated. A deep understanding of their spatiotemporal regulation is needed to understand their contribution to physiology and pathology. Here, we have characterized the spatiotemporal expression pattern of hnRNP A1 and its splice variant hnRNP A1B in mice. We have found that hnRNP A1B expression is more restricted to the CNS compared to hnRNP A1, and that it can form an SDS-resistant dimer in the CNS. Also, hnRNP A1B expression becomes progressively restricted to motor neurons in the ventral horn of the spinal cord, compared to hnRNP A1 which is more broadly expressed. We also demonstrate that hnRNP A1B is present in neuronal processes, while hnRNP A1 is absent. This finding supports a hypothesis that hnRNP A1B may have a cytosolic function in neurons that is not shared with hnRNP A1. Our results demonstrate that both isoforms are differentially expressed across tissues and have distinct localization profiles, suggesting that the two isoforms may have specific subcellular functions that can uniquely contribute to disease progression.https://www.frontiersin.org/articles/10.3389/fnins.2021.724307/fullRNA binding proteincentral nervous systemmotor neuronamyotrophic lateral sclerosis (ALS)hnRNP |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Myriam Gagné Myriam Gagné Jade-Emmanuelle Deshaies Hadjara Sidibé Hadjara Sidibé Yousri Benchaar Danielle Arbour Alicia Dubinski Alicia Dubinski Gurleen Litt Sarah Peyrard Richard Robitaille Chantelle F. Sephton Christine Vande Velde Christine Vande Velde |
spellingShingle |
Myriam Gagné Myriam Gagné Jade-Emmanuelle Deshaies Hadjara Sidibé Hadjara Sidibé Yousri Benchaar Danielle Arbour Alicia Dubinski Alicia Dubinski Gurleen Litt Sarah Peyrard Richard Robitaille Chantelle F. Sephton Christine Vande Velde Christine Vande Velde hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated Frontiers in Neuroscience RNA binding protein central nervous system motor neuron amyotrophic lateral sclerosis (ALS) hnRNP |
author_facet |
Myriam Gagné Myriam Gagné Jade-Emmanuelle Deshaies Hadjara Sidibé Hadjara Sidibé Yousri Benchaar Danielle Arbour Alicia Dubinski Alicia Dubinski Gurleen Litt Sarah Peyrard Richard Robitaille Chantelle F. Sephton Christine Vande Velde Christine Vande Velde |
author_sort |
Myriam Gagné |
title |
hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated |
title_short |
hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated |
title_full |
hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated |
title_fullStr |
hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated |
title_full_unstemmed |
hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated |
title_sort |
hnrnp a1b, a splice variant of hnrnpa1, is spatially and temporally regulated |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroscience |
issn |
1662-453X |
publishDate |
2021-09-01 |
description |
RNA binding proteins (RBPs) play a key role in cellular growth, homoeostasis and survival and are tightly regulated. A deep understanding of their spatiotemporal regulation is needed to understand their contribution to physiology and pathology. Here, we have characterized the spatiotemporal expression pattern of hnRNP A1 and its splice variant hnRNP A1B in mice. We have found that hnRNP A1B expression is more restricted to the CNS compared to hnRNP A1, and that it can form an SDS-resistant dimer in the CNS. Also, hnRNP A1B expression becomes progressively restricted to motor neurons in the ventral horn of the spinal cord, compared to hnRNP A1 which is more broadly expressed. We also demonstrate that hnRNP A1B is present in neuronal processes, while hnRNP A1 is absent. This finding supports a hypothesis that hnRNP A1B may have a cytosolic function in neurons that is not shared with hnRNP A1. Our results demonstrate that both isoforms are differentially expressed across tissues and have distinct localization profiles, suggesting that the two isoforms may have specific subcellular functions that can uniquely contribute to disease progression. |
topic |
RNA binding protein central nervous system motor neuron amyotrophic lateral sclerosis (ALS) hnRNP |
url |
https://www.frontiersin.org/articles/10.3389/fnins.2021.724307/full |
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