Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells
Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step in adipocyte lipolysis. The activity of HSL is thought to be primarily regulated by reversible phosphorylation. However, the regulation of HSL activity by pretranslational mechanisms has been poorly studied. The present studies were und...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
1998-08-01
|
Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520321994 |
id |
doaj-2480a4b3323b4aa89f8876426185e4c4 |
---|---|
record_format |
Article |
spelling |
doaj-2480a4b3323b4aa89f8876426185e4c42021-04-26T13:50:07ZengElsevierJournal of Lipid Research0022-22751998-08-0139816881695Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cellsValérie Large0Peter Arner1Signy Reynisdottir2Jacques Grober3Vanessa Van Harmelen4Cecilia Holm5Dominique Langin6To whom correspondence should be addressed.; Department of Medicine, Karolinska Institute, Huddinge University Hospital, Stockholm, SwedenDepartment of Medicine, Karolinska Institute, Huddinge University Hospital, Stockholm, SwedenDepartment of Medicine, Karolinska Institute, Huddinge University Hospital, Stockholm, SwedenINSERM U137, Institut Louis Bugnard, CHU Rangueil, Toulouse, FranceDepartment of Medicine, Karolinska Institute, Huddinge University Hospital, Stockholm, SwedenDepartment of Cell and Molecular Biology, Lund University, Lund, SwedenINSERM U137, Institut Louis Bugnard, CHU Rangueil, Toulouse, FranceHormone-sensitive lipase (HSL) catalyzes the rate-limiting step in adipocyte lipolysis. The activity of HSL is thought to be primarily regulated by reversible phosphorylation. However, the regulation of HSL activity by pretranslational mechanisms has been poorly studied. The present studies were undertaken to explore the relationship between the levels of HSL protein and mRNA expressions and the lipolytic capacity. The study was performed in human abdominal subcutaneous adipocytes with identical sizes but having either a high (HL) or low (LL) lipolytic capacity (n = 16). Basal and maximal lipolysis induced by catecholamines, an adenylyl cyclase activator forskolin, and a cyclic AMP analogue dibutyryl cAMP were 50% lower in LL- in comparison with HL-fat cells (P < 0.05 or better). No differences in drug sensitivity were found. HSL activity and quantity were about 50% lower in LL- compared with HL-fat cells (P < 0.05). Moreover, the mRNA ratio between HSL and γ-actin was 35% lower in LL-compared with HL-fat cells (P < 0.05). There was a strong linear correlation between the protein and enzymatic HSL measurements (r2 = 0.91). In addition, the maximum lipolytic capacity was significantly correlated with HSL activity (r 2 = 0.75) and HSL protein amount (r 2 = 0.64). It is concluded that hormone-sensitive lipase (HSL) expression, measured either as total HSL protein by Western blot analysis or as total amount of activatable HSL enzyme, is a major determinant of the maximum lipolytic capacity of human fat cells. In addition, HSL protein expression is at least, in part, determined by HSL mRNA expression.—Large, V., P. Arner, S. Reynisdottir, J. Grober, V. Van Harmelen, C. Holm, and D. Langin. Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells.http://www.sciencedirect.com/science/article/pii/S0022227520321994β-adrenoceptorscatecholamineswhite adipose tissueobesitylipid mobilization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valérie Large Peter Arner Signy Reynisdottir Jacques Grober Vanessa Van Harmelen Cecilia Holm Dominique Langin |
spellingShingle |
Valérie Large Peter Arner Signy Reynisdottir Jacques Grober Vanessa Van Harmelen Cecilia Holm Dominique Langin Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells Journal of Lipid Research β-adrenoceptors catecholamines white adipose tissue obesity lipid mobilization |
author_facet |
Valérie Large Peter Arner Signy Reynisdottir Jacques Grober Vanessa Van Harmelen Cecilia Holm Dominique Langin |
author_sort |
Valérie Large |
title |
Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells |
title_short |
Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells |
title_full |
Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells |
title_fullStr |
Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells |
title_full_unstemmed |
Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells |
title_sort |
hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1998-08-01 |
description |
Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step in adipocyte lipolysis. The activity of HSL is thought to be primarily regulated by reversible phosphorylation. However, the regulation of HSL activity by pretranslational mechanisms has been poorly studied. The present studies were undertaken to explore the relationship between the levels of HSL protein and mRNA expressions and the lipolytic capacity. The study was performed in human abdominal subcutaneous adipocytes with identical sizes but having either a high (HL) or low (LL) lipolytic capacity (n = 16). Basal and maximal lipolysis induced by catecholamines, an adenylyl cyclase activator forskolin, and a cyclic AMP analogue dibutyryl cAMP were 50% lower in LL- in comparison with HL-fat cells (P < 0.05 or better). No differences in drug sensitivity were found. HSL activity and quantity were about 50% lower in LL- compared with HL-fat cells (P < 0.05). Moreover, the mRNA ratio between HSL and γ-actin was 35% lower in LL-compared with HL-fat cells (P < 0.05). There was a strong linear correlation between the protein and enzymatic HSL measurements (r2 = 0.91). In addition, the maximum lipolytic capacity was significantly correlated with HSL activity (r 2 = 0.75) and HSL protein amount (r 2 = 0.64). It is concluded that hormone-sensitive lipase (HSL) expression, measured either as total HSL protein by Western blot analysis or as total amount of activatable HSL enzyme, is a major determinant of the maximum lipolytic capacity of human fat cells. In addition, HSL protein expression is at least, in part, determined by HSL mRNA expression.—Large, V., P. Arner, S. Reynisdottir, J. Grober, V. Van Harmelen, C. Holm, and D. Langin. Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells. |
topic |
β-adrenoceptors catecholamines white adipose tissue obesity lipid mobilization |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520321994 |
work_keys_str_mv |
AT valerielarge hormonesensitivelipaseexpressionandactivityinrelationtolipolysisinhumanfatcells AT peterarner hormonesensitivelipaseexpressionandactivityinrelationtolipolysisinhumanfatcells AT signyreynisdottir hormonesensitivelipaseexpressionandactivityinrelationtolipolysisinhumanfatcells AT jacquesgrober hormonesensitivelipaseexpressionandactivityinrelationtolipolysisinhumanfatcells AT vanessavanharmelen hormonesensitivelipaseexpressionandactivityinrelationtolipolysisinhumanfatcells AT ceciliaholm hormonesensitivelipaseexpressionandactivityinrelationtolipolysisinhumanfatcells AT dominiquelangin hormonesensitivelipaseexpressionandactivityinrelationtolipolysisinhumanfatcells |
_version_ |
1721507546889453568 |