ETHANOL PRECIPITATION OF GLYCOSYL HYDROLASES PRODUCED BY Trichoderma harzianum P49P11

• Main Authors: M. A. Mariño, S. Freitas, E. A. Miranda
• Format: Article
• Language: English
• Published: Brazilian Society of Chemical Engineering 2015-06-01
• Series: Brazilian Journal of Chemical Engineering
• Subjects: Trichoderma harzianum; Glycosyl hydrolases; Ethanol precipitation
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322015000200325&lng=en&tlng=en

AbstractThis study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regard less of the temperature (5.0, 15.0, or 25.0 °C). The maximum recovery of cellulase activity as FPase was 77% by precipitation carried out at this same pH and ethanol concentration but at 5.0 °C. Therefore, ethanol precipitation can be considered to be an efficient technique for xylanase concentration and, to a certain extent, also for the cellulase complex.