Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: Application to therapeutic drug monitoring
Antiepileptic drugs are the first choice for epilepsy treatment. Monitoring antiepileptic drugs is important to minimize their adverse side effects by choosing the optimum drug dosage. An accurate and high throughput supercritical fluid chromatography-tandem mass spectrometry method has been develop...
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doaj-248669c9b7524643988537eee975213d2020-11-25T01:11:16ZengElsevierArabian Journal of Chemistry1878-53522019-12-0112847754783Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: Application to therapeutic drug monitoringLi Wang0Jingting Wang1Jing Zhang2Qikun Jiang3Longshan Zhao4Tianhong Zhang5School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaDepartment of Pharmacy, Fuzhou General Hospital of Nanjing Command PLA, Fuzhou 350025, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; Corresponding authors at: Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110016, China. Fax: +86 24 23986321.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; Corresponding authors at: Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110016, China. Fax: +86 24 23986321.Antiepileptic drugs are the first choice for epilepsy treatment. Monitoring antiepileptic drugs is important to minimize their adverse side effects by choosing the optimum drug dosage. An accurate and high throughput supercritical fluid chromatography-tandem mass spectrometry method has been developed for the simultaneous quantification of several antiepileptic drugs in human plasma. Plasma samples were extracted with ethyl acetate and the upper organic layer was directly injected into the supercritical fluid chromatography/mass spectrometry (SFC-MS/MS) system without further nitrogen evaporation and subsequent reconstitution. The analytes were eluted on a UPC2TM BEH, 2-EP column (100 × 3 mm, 1.7 μm) at a flow rate of 1.0 mL/min and multi-reaction monitoring (MRM) was performed for determination of the analytes and internal standard (IS) in polarity switching mode. Calibration curves were linear over the concentration ranges of 0.08–40, 0.01–15, 0.01–8 and 0.5–50 μg/mL with lower limit of quantifications of 0.08, 0.01, 0.01 and 0.50 μg/mL for topiramate, carbamazepine, oxcarbazepine and monohydroxycarbamazepine, respectively. This sensitive, accurate, novel method will be very useful for monitoring the above antiepileptic drugs and for pharmacokinetic studies. Keywords: Antiepileptic drugs, SFC-ESI-MS/MS, Human plasma, Therapeutic drug monitoringhttp://www.sciencedirect.com/science/article/pii/S1878535216301605 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Wang Jingting Wang Jing Zhang Qikun Jiang Longshan Zhao Tianhong Zhang |
spellingShingle |
Li Wang Jingting Wang Jing Zhang Qikun Jiang Longshan Zhao Tianhong Zhang Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: Application to therapeutic drug monitoring Arabian Journal of Chemistry |
author_facet |
Li Wang Jingting Wang Jing Zhang Qikun Jiang Longshan Zhao Tianhong Zhang |
author_sort |
Li Wang |
title |
Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: Application to therapeutic drug monitoring |
title_short |
Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: Application to therapeutic drug monitoring |
title_full |
Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: Application to therapeutic drug monitoring |
title_fullStr |
Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: Application to therapeutic drug monitoring |
title_full_unstemmed |
Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: Application to therapeutic drug monitoring |
title_sort |
simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by sfc-esi-ms/ms with polarity switching: application to therapeutic drug monitoring |
publisher |
Elsevier |
series |
Arabian Journal of Chemistry |
issn |
1878-5352 |
publishDate |
2019-12-01 |
description |
Antiepileptic drugs are the first choice for epilepsy treatment. Monitoring antiepileptic drugs is important to minimize their adverse side effects by choosing the optimum drug dosage. An accurate and high throughput supercritical fluid chromatography-tandem mass spectrometry method has been developed for the simultaneous quantification of several antiepileptic drugs in human plasma. Plasma samples were extracted with ethyl acetate and the upper organic layer was directly injected into the supercritical fluid chromatography/mass spectrometry (SFC-MS/MS) system without further nitrogen evaporation and subsequent reconstitution. The analytes were eluted on a UPC2TM BEH, 2-EP column (100 × 3 mm, 1.7 μm) at a flow rate of 1.0 mL/min and multi-reaction monitoring (MRM) was performed for determination of the analytes and internal standard (IS) in polarity switching mode. Calibration curves were linear over the concentration ranges of 0.08–40, 0.01–15, 0.01–8 and 0.5–50 μg/mL with lower limit of quantifications of 0.08, 0.01, 0.01 and 0.50 μg/mL for topiramate, carbamazepine, oxcarbazepine and monohydroxycarbamazepine, respectively. This sensitive, accurate, novel method will be very useful for monitoring the above antiepileptic drugs and for pharmacokinetic studies. Keywords: Antiepileptic drugs, SFC-ESI-MS/MS, Human plasma, Therapeutic drug monitoring |
url |
http://www.sciencedirect.com/science/article/pii/S1878535216301605 |
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