Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from War-Injured Patients from the Eastern Ukraine
Recently, a total of 32 carbapenem- and fluoroquinolone-resistant <i>Acinetobacter baumannii</i> (Ab) isolates was isolated from war-injured patients who were treated at German Bundeswehr Hospitals, and preliminarily typed by “DiversiLab” repetitive elements sequence-based (rep-) PCR. Co...
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doaj-249d8b9d4ff04eadb8855a021a9a85db2020-11-25T01:50:13ZengMDPI AGAntibiotics2079-63822020-09-01957957910.3390/antibiotics9090579Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from War-Injured Patients from the Eastern UkrainePaul G. Higgins0Ralf Matthias Hagen1Andreas Podbielski2Hagen Frickmann3Philipp Warnke4Institute for Medical Microbiology, Immunology, and Hygiene, University of Cologne, 50935 Cologne, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, GermanyInstitute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, GermanyInstitute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, GermanyInstitute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, GermanyRecently, a total of 32 carbapenem- and fluoroquinolone-resistant <i>Acinetobacter baumannii</i> (Ab) isolates was isolated from war-injured patients who were treated at German Bundeswehr Hospitals, and preliminarily typed by “DiversiLab” repetitive elements sequence-based (rep-) PCR. Core genome-based sequence typing was also used to provide more detailed epidemiological information. From the clusters observed by rep-PCR, selected Ab strains were subjected to Next Generation Sequencing (NGS) in order to compare them with international outbreak-associated Ab strains and to identify MLST (multi-locus sequence type) lineages, as well as to identify known resistance genes. Accordingly, NGS indicated higher diversity than rep-PCR, but also confirmed likely transmission events. The identified acquired carbapenem-resistant genes comprised <i>bla</i><sub>OXA-23</sub>, <i>bla</i><sub>OXA-72</sub> and <i>bla</i><sub>GES-12</sub>, as well as various other intrinsic and acquired resistance-associated genetic elements. All isolates clustered with the previously identified international clonal lineages IC1, IC2, IC6 and IC7, with corresponding Pasteur sequence types ST1, ST2, ST78 and ST25, respectively. In conclusion, the assessment confirmed a broad spectrum of resistance-associated genes in Ab isolated from war-injured patients from the Eastern Ukraine, and provided the first insights into locally abundant clonal lineages.https://www.mdpi.com/2079-6382/9/9/579<i>Acinetobacter baumannii</i> complexwar injuryUkraineepidemiologysequence typingcarbapenem resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paul G. Higgins Ralf Matthias Hagen Andreas Podbielski Hagen Frickmann Philipp Warnke |
spellingShingle |
Paul G. Higgins Ralf Matthias Hagen Andreas Podbielski Hagen Frickmann Philipp Warnke Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from War-Injured Patients from the Eastern Ukraine Antibiotics <i>Acinetobacter baumannii</i> complex war injury Ukraine epidemiology sequence typing carbapenem resistance |
author_facet |
Paul G. Higgins Ralf Matthias Hagen Andreas Podbielski Hagen Frickmann Philipp Warnke |
author_sort |
Paul G. Higgins |
title |
Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from War-Injured Patients from the Eastern Ukraine |
title_short |
Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from War-Injured Patients from the Eastern Ukraine |
title_full |
Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from War-Injured Patients from the Eastern Ukraine |
title_fullStr |
Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from War-Injured Patients from the Eastern Ukraine |
title_full_unstemmed |
Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from War-Injured Patients from the Eastern Ukraine |
title_sort |
molecular epidemiology of carbapenem-resistant <i>acinetobacter baumannii</i> isolated from war-injured patients from the eastern ukraine |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2020-09-01 |
description |
Recently, a total of 32 carbapenem- and fluoroquinolone-resistant <i>Acinetobacter baumannii</i> (Ab) isolates was isolated from war-injured patients who were treated at German Bundeswehr Hospitals, and preliminarily typed by “DiversiLab” repetitive elements sequence-based (rep-) PCR. Core genome-based sequence typing was also used to provide more detailed epidemiological information. From the clusters observed by rep-PCR, selected Ab strains were subjected to Next Generation Sequencing (NGS) in order to compare them with international outbreak-associated Ab strains and to identify MLST (multi-locus sequence type) lineages, as well as to identify known resistance genes. Accordingly, NGS indicated higher diversity than rep-PCR, but also confirmed likely transmission events. The identified acquired carbapenem-resistant genes comprised <i>bla</i><sub>OXA-23</sub>, <i>bla</i><sub>OXA-72</sub> and <i>bla</i><sub>GES-12</sub>, as well as various other intrinsic and acquired resistance-associated genetic elements. All isolates clustered with the previously identified international clonal lineages IC1, IC2, IC6 and IC7, with corresponding Pasteur sequence types ST1, ST2, ST78 and ST25, respectively. In conclusion, the assessment confirmed a broad spectrum of resistance-associated genes in Ab isolated from war-injured patients from the Eastern Ukraine, and provided the first insights into locally abundant clonal lineages. |
topic |
<i>Acinetobacter baumannii</i> complex war injury Ukraine epidemiology sequence typing carbapenem resistance |
url |
https://www.mdpi.com/2079-6382/9/9/579 |
work_keys_str_mv |
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