Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain
While the role of circulating ouabain-like compounds in the cardiovascular and central nervous systems, kidney and other tissues in health and disease is well documented, little is known about its effects in skeletal muscle. In this study, rats were intraperitoneally injected with ouabain (0.1–10 µg...
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doaj-24a01b0695824c38899e76b73214e8ec2020-11-25T02:33:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01212875287510.3390/ijms21082875Skeletal Muscle Na,K-ATPase as a Target for Circulating OuabainVioletta V. Kravtsova0Elena V. Bouzinova1Vladimir V. Matchkov2Igor I. Krivoi3Department of General Physiology, St. Petersburg State University, St. Petersburg 199034, RussiaDepartment of Biomedicine, University of Aarhus, Aarhus C 8000, DenmarkDepartment of Biomedicine, University of Aarhus, Aarhus C 8000, DenmarkDepartment of General Physiology, St. Petersburg State University, St. Petersburg 199034, RussiaWhile the role of circulating ouabain-like compounds in the cardiovascular and central nervous systems, kidney and other tissues in health and disease is well documented, little is known about its effects in skeletal muscle. In this study, rats were intraperitoneally injected with ouabain (0.1–10 µg/kg for 4 days) alone or with subsequent injections of lipopolysaccharide (1 mg/kg). Some rats were also subjected to disuse for 6 h by hindlimb suspension. In the diaphragm muscle, chronic ouabain (1 µg/kg) hyperpolarized resting potential of extrajunctional membrane due to specific increase in electrogenic transport activity of the 2 Na,K-ATPase isozyme and without changes in 1 and 2 Na,K-ATPase protein content. Ouabain (10–20 nM), acutely applied to isolated intact diaphragm muscle from not injected rats, hyperpolarized the membrane to a similar extent. Chronic ouabain administration prevented lipopolysaccharide-induced (diaphragm muscle) or disuse-induced (soleus muscle) depolarization of the extrajunctional membrane. No stimulation of the 1 Na,K-ATPase activity in human red blood cells, purified lamb kidney and <i>Torpedo</i> membrane preparations by low ouabain concentrations was observed. Our results suggest that skeletal muscle electrogenesis is subjected to regulation by circulating ouabain via the 2 Na,K-ATPase isozyme that could be important for adaptation of this tissue to functional impairment.https://www.mdpi.com/1422-0067/21/8/2875skeletal muscleNa,K-ATPase isozymesouabainresting membrane potential |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Violetta V. Kravtsova Elena V. Bouzinova Vladimir V. Matchkov Igor I. Krivoi |
spellingShingle |
Violetta V. Kravtsova Elena V. Bouzinova Vladimir V. Matchkov Igor I. Krivoi Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain International Journal of Molecular Sciences skeletal muscle Na,K-ATPase isozymes ouabain resting membrane potential |
author_facet |
Violetta V. Kravtsova Elena V. Bouzinova Vladimir V. Matchkov Igor I. Krivoi |
author_sort |
Violetta V. Kravtsova |
title |
Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain |
title_short |
Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain |
title_full |
Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain |
title_fullStr |
Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain |
title_full_unstemmed |
Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain |
title_sort |
skeletal muscle na,k-atpase as a target for circulating ouabain |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-04-01 |
description |
While the role of circulating ouabain-like compounds in the cardiovascular and central nervous systems, kidney and other tissues in health and disease is well documented, little is known about its effects in skeletal muscle. In this study, rats were intraperitoneally injected with ouabain (0.1–10 µg/kg for 4 days) alone or with subsequent injections of lipopolysaccharide (1 mg/kg). Some rats were also subjected to disuse for 6 h by hindlimb suspension. In the diaphragm muscle, chronic ouabain (1 µg/kg) hyperpolarized resting potential of extrajunctional membrane due to specific increase in electrogenic transport activity of the 2 Na,K-ATPase isozyme and without changes in 1 and 2 Na,K-ATPase protein content. Ouabain (10–20 nM), acutely applied to isolated intact diaphragm muscle from not injected rats, hyperpolarized the membrane to a similar extent. Chronic ouabain administration prevented lipopolysaccharide-induced (diaphragm muscle) or disuse-induced (soleus muscle) depolarization of the extrajunctional membrane. No stimulation of the 1 Na,K-ATPase activity in human red blood cells, purified lamb kidney and <i>Torpedo</i> membrane preparations by low ouabain concentrations was observed. Our results suggest that skeletal muscle electrogenesis is subjected to regulation by circulating ouabain via the 2 Na,K-ATPase isozyme that could be important for adaptation of this tissue to functional impairment. |
topic |
skeletal muscle Na,K-ATPase isozymes ouabain resting membrane potential |
url |
https://www.mdpi.com/1422-0067/21/8/2875 |
work_keys_str_mv |
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