Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to <i>Verticillium wilt</i>
The Negative on TATA-less (NOT) 2_3_5 domain proteins play key roles in mRNA metabolism and transcription regulation, but few comprehensive studies have focused on this protein family in plants. In our study, a total of 30 <i>NOT2_3_5</i> genes were identified in four cotton genomes: <...
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doaj-24b380f29d5b49c9a1f510ceaa57a3e82021-06-01T01:08:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01225634563410.3390/ijms22115634Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to <i>Verticillium wilt</i>Pei Zhao0Tengfei Qin1Wei Chen2Xiaohui Sang3Yunlei Zhao4Hongmei Wang5State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaHenan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Sciences and Technology, Xinxiang 453003, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaThe Negative on TATA-less (NOT) 2_3_5 domain proteins play key roles in mRNA metabolism and transcription regulation, but few comprehensive studies have focused on this protein family in plants. In our study, a total of 30 <i>NOT2_3_5</i> genes were identified in four cotton genomes: <i>Gossypium. arboretum</i>, <i>G. raimondii</i>, <i>G. hirsutum</i> and <i>G. barbadense.</i> Phylogenetic analysis showed that all the NOT2_3_5 domain proteins were divided into two classes. The <i>NOT2_3_5</i> genes were expanded frequently, and segmental duplication had significant effects in their expansion process. The <i>cis</i>-regulatory elements analysis of <i>NOT2_3_5</i> promoter regions indicated that NOT2_3_5 domain proteins might participate in plant growth and development processes and responds to exogenous stimuli. Expression patterns demonstrated that all of the <i>GhNOT2_3_5</i> genes were expressed in the majority of tissues and fiber development stages, and that these genes were induced by multiple stresses. Quantitative real-time PCR showed that <i>GbNOT2_3_5</i> genes were up-regulated in response to verticillium wilt and the silencing of <i>GbNOT2_3_5-3/8</i> and <i>GbNOT2_3_5-4/9</i> led to more susceptibility to verticillium wilt than controls. Identification and analysis of the NOT2_3_5 protein family will be beneficial for further research on their biological functions.https://www.mdpi.com/1422-0067/22/11/5634NOT2_3_5 domain protein<i>Verticillium wilt</i>cotton |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pei Zhao Tengfei Qin Wei Chen Xiaohui Sang Yunlei Zhao Hongmei Wang |
spellingShingle |
Pei Zhao Tengfei Qin Wei Chen Xiaohui Sang Yunlei Zhao Hongmei Wang Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to <i>Verticillium wilt</i> International Journal of Molecular Sciences NOT2_3_5 domain protein <i>Verticillium wilt</i> cotton |
author_facet |
Pei Zhao Tengfei Qin Wei Chen Xiaohui Sang Yunlei Zhao Hongmei Wang |
author_sort |
Pei Zhao |
title |
Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to <i>Verticillium wilt</i> |
title_short |
Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to <i>Verticillium wilt</i> |
title_full |
Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to <i>Verticillium wilt</i> |
title_fullStr |
Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to <i>Verticillium wilt</i> |
title_full_unstemmed |
Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to <i>Verticillium wilt</i> |
title_sort |
genome-wide study of not2_3_5 protein subfamily in cotton and their necessity in resistance to <i>verticillium wilt</i> |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-05-01 |
description |
The Negative on TATA-less (NOT) 2_3_5 domain proteins play key roles in mRNA metabolism and transcription regulation, but few comprehensive studies have focused on this protein family in plants. In our study, a total of 30 <i>NOT2_3_5</i> genes were identified in four cotton genomes: <i>Gossypium. arboretum</i>, <i>G. raimondii</i>, <i>G. hirsutum</i> and <i>G. barbadense.</i> Phylogenetic analysis showed that all the NOT2_3_5 domain proteins were divided into two classes. The <i>NOT2_3_5</i> genes were expanded frequently, and segmental duplication had significant effects in their expansion process. The <i>cis</i>-regulatory elements analysis of <i>NOT2_3_5</i> promoter regions indicated that NOT2_3_5 domain proteins might participate in plant growth and development processes and responds to exogenous stimuli. Expression patterns demonstrated that all of the <i>GhNOT2_3_5</i> genes were expressed in the majority of tissues and fiber development stages, and that these genes were induced by multiple stresses. Quantitative real-time PCR showed that <i>GbNOT2_3_5</i> genes were up-regulated in response to verticillium wilt and the silencing of <i>GbNOT2_3_5-3/8</i> and <i>GbNOT2_3_5-4/9</i> led to more susceptibility to verticillium wilt than controls. Identification and analysis of the NOT2_3_5 protein family will be beneficial for further research on their biological functions. |
topic |
NOT2_3_5 domain protein <i>Verticillium wilt</i> cotton |
url |
https://www.mdpi.com/1422-0067/22/11/5634 |
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