NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli
Abstract Noncoding RNAs (ncRNAs) can finely control the expression of target genes at the posttranscriptional level in prokaryotes. Regulatory small RNAs (sRNAs) designed to control target gene expression for applications in metabolic engineering and synthetic biology have been successfully develope...
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doaj-24b8ce7a231f4b4cb8b94d155f1c7c7c2020-11-25T03:13:17ZengSpringerOpenAMB Express2191-08552019-09-019111010.1186/s13568-019-0881-7NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coliGuihua Hu0Tao Hu1Yuhua Zhan2Wei Lu3Min Lin4Yunhong Huang5Yongliang Yan6College of Life Sciences, Jiangxi Normal UniversityBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Institute, Chinese Academy of Agricultural SciencesCollege of Life Sciences, Jiangxi Normal UniversityBiotechnology Research Institute, Chinese Academy of Agricultural SciencesAbstract Noncoding RNAs (ncRNAs) can finely control the expression of target genes at the posttranscriptional level in prokaryotes. Regulatory small RNAs (sRNAs) designed to control target gene expression for applications in metabolic engineering and synthetic biology have been successfully developed and used. However, the effect on the heterologous expression of species- or strain-specific ncRNAs in other bacterial strains remains poorly understood. In this work, a Pseudomonas stutzeri species-specific regulatory ncRNA, NfiS, which has been shown to play an important role in the response to oxidative stress as well as osmotic stress in P. stutzeri A1501, was cloned and transferred to the Escherichia coli strain Trans10. Recombinant NfiS-expressing E. coli, namely, Trans10-nfiS, exhibited significant enhancement of tolerance to oxidative stress. To map the possible gene regulatory networks mediated by NfiS in E. coli under oxidative stress, a microarray assay was performed to delineate the transcriptomic differences between Trans10-nfiS and wild-type E. coli under H2O2 shock treatment conditions. In all, 1184 genes were found to be significantly altered, and these genes were divided into mainly five functional categories: stress response, regulation, metabolism related, transport or membrane protein and unknown function. Our results suggest that the P. stutzeri species-specific ncRNA NfiS acts as a regulator that integrates adaptation to H2O2 with other cellular stress responses and helps protect E. coli cells against oxidative damage.http://link.springer.com/article/10.1186/s13568-019-0881-7Pseudomonas stutzeri A1501NfiSRecombinant Escherichia coliOxidative stressRegulatory element |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guihua Hu Tao Hu Yuhua Zhan Wei Lu Min Lin Yunhong Huang Yongliang Yan |
spellingShingle |
Guihua Hu Tao Hu Yuhua Zhan Wei Lu Min Lin Yunhong Huang Yongliang Yan NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli AMB Express Pseudomonas stutzeri A1501 NfiS Recombinant Escherichia coli Oxidative stress Regulatory element |
author_facet |
Guihua Hu Tao Hu Yuhua Zhan Wei Lu Min Lin Yunhong Huang Yongliang Yan |
author_sort |
Guihua Hu |
title |
NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli |
title_short |
NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli |
title_full |
NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli |
title_fullStr |
NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli |
title_full_unstemmed |
NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli |
title_sort |
nfis, a species-specific regulatory noncoding rna of pseudomonas stutzeri, enhances oxidative stress tolerance in escherichia coli |
publisher |
SpringerOpen |
series |
AMB Express |
issn |
2191-0855 |
publishDate |
2019-09-01 |
description |
Abstract Noncoding RNAs (ncRNAs) can finely control the expression of target genes at the posttranscriptional level in prokaryotes. Regulatory small RNAs (sRNAs) designed to control target gene expression for applications in metabolic engineering and synthetic biology have been successfully developed and used. However, the effect on the heterologous expression of species- or strain-specific ncRNAs in other bacterial strains remains poorly understood. In this work, a Pseudomonas stutzeri species-specific regulatory ncRNA, NfiS, which has been shown to play an important role in the response to oxidative stress as well as osmotic stress in P. stutzeri A1501, was cloned and transferred to the Escherichia coli strain Trans10. Recombinant NfiS-expressing E. coli, namely, Trans10-nfiS, exhibited significant enhancement of tolerance to oxidative stress. To map the possible gene regulatory networks mediated by NfiS in E. coli under oxidative stress, a microarray assay was performed to delineate the transcriptomic differences between Trans10-nfiS and wild-type E. coli under H2O2 shock treatment conditions. In all, 1184 genes were found to be significantly altered, and these genes were divided into mainly five functional categories: stress response, regulation, metabolism related, transport or membrane protein and unknown function. Our results suggest that the P. stutzeri species-specific ncRNA NfiS acts as a regulator that integrates adaptation to H2O2 with other cellular stress responses and helps protect E. coli cells against oxidative damage. |
topic |
Pseudomonas stutzeri A1501 NfiS Recombinant Escherichia coli Oxidative stress Regulatory element |
url |
http://link.springer.com/article/10.1186/s13568-019-0881-7 |
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