Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.

Methods currently in use for the quantitative measurement of sulfated serum bile acids usually lead to low recoveries. The present technique uses two internal standards and involves enzymatic hydrolysis followed by extraction of the non-sulfated fraction in ether at pH 4.6. The sulfated fraction is...

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Main Authors:	G Lepage, C C Roy, A M Weber
Format:	Article
Language:	English
Published:	Elsevier 1981-05-01
Series:	Journal of Lipid Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S002222752037382X

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spelling	doaj-24d761e20fe84f48a7f18ffe88d3a5e72021-04-24T05:50:16ZengElsevierJournal of Lipid Research0022-22751981-05-01224705711Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.G LepageC C RoyA M WeberMethods currently in use for the quantitative measurement of sulfated serum bile acids usually lead to low recoveries. The present technique uses two internal standards and involves enzymatic hydrolysis followed by extraction of the non-sulfated fraction in ether at pH 4.6. The sulfated fraction is then solvolysed before gas-liquid chromatographic analysis of the individual bile acids of both fractions. Using 200 microliters of serum, recoveries of more than 81% of sulfated bile acids were achieved. This method is of particular significance in view of recent evidence showing that certain sulfated bile acids are cholestatic.http://www.sciencedirect.com/science/article/pii/S002222752037382X
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	G Lepage C C Roy A M Weber
spellingShingle	G Lepage C C Roy A M Weber Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns. Journal of Lipid Research
author_facet	G Lepage C C Roy A M Weber
author_sort	G Lepage
title	Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.
title_short	Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.
title_full	Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.
title_fullStr	Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.
title_full_unstemmed	Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.
title_sort	separation of sulfated from non-sulfated serum bile acids without the use of sephadex columns.
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1981-05-01
description	Methods currently in use for the quantitative measurement of sulfated serum bile acids usually lead to low recoveries. The present technique uses two internal standards and involves enzymatic hydrolysis followed by extraction of the non-sulfated fraction in ether at pH 4.6. The sulfated fraction is then solvolysed before gas-liquid chromatographic analysis of the individual bile acids of both fractions. Using 200 microliters of serum, recoveries of more than 81% of sulfated bile acids were achieved. This method is of particular significance in view of recent evidence showing that certain sulfated bile acids are cholestatic.
url	http://www.sciencedirect.com/science/article/pii/S002222752037382X
work_keys_str_mv	AT glepage separationofsulfatedfromnonsulfatedserumbileacidswithouttheuseofsephadexcolumns AT ccroy separationofsulfatedfromnonsulfatedserumbileacidswithouttheuseofsephadexcolumns AT amweber separationofsulfatedfromnonsulfatedserumbileacidswithouttheuseofsephadexcolumns
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Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.

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