Biofilm-Forming Potential of Ocular Fluid <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> on Ex Vivo Human Corneas from Attachment to Dispersal Phase
The biofilm-forming potential of <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>, isolated from patients with Endophthalmitis, was monitored using glass cover slips and cadaveric corneas as substrata. Both the ocular fluid isolates exhibited biofilm-forming...
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doaj-24fa760255ee462e926a772c241be30c2021-06-01T00:49:11ZengMDPI AGMicroorganisms2076-26072021-05-0191124112410.3390/microorganisms9061124Biofilm-Forming Potential of Ocular Fluid <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> on Ex Vivo Human Corneas from Attachment to Dispersal PhaseRanjith Konduri0Chinthala Reddy Saiabhilash1Sisinthy Shivaji2Jhaveri Microbiology Centre, Prof. Brien Holden Eye Research Centre, Kallam Anji Reddy Campus, L. V. Prasad Eye Institute, Hyderabad 500034, Telangana, IndiaJhaveri Microbiology Centre, Prof. Brien Holden Eye Research Centre, Kallam Anji Reddy Campus, L. V. Prasad Eye Institute, Hyderabad 500034, Telangana, IndiaJhaveri Microbiology Centre, Prof. Brien Holden Eye Research Centre, Kallam Anji Reddy Campus, L. V. Prasad Eye Institute, Hyderabad 500034, Telangana, IndiaThe biofilm-forming potential of <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>, isolated from patients with Endophthalmitis, was monitored using glass cover slips and cadaveric corneas as substrata. Both the ocular fluid isolates exhibited biofilm-forming potential by the Congo red agar, Crystal violet and 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H-tetra-zolium hydroxide (XTT) methods. Confocal microscopy demonstrated that the thickness of the biofilm increased from 4–120 h of biofilm formation. Scanning electron microscopic studies indicated that the biofilms grown on cover slips and ex vivo corneas of both the isolates go through an adhesion phase at 4 h followed by multilayer clumping of cells with intercellular connections and copious amounts of extracellular polymeric substance. Clumps subsequently formed columns and eventually single cells were visible indicative of dispersal phase. Biofilm formation was more rapid when the cornea was used as a substratum. In the biofilms grown on corneas, clumping of cells, formation of 3D structures and final appearance of single cells indicative of dispersal phase occurred by 48 h compared to 96–120 h when biofilms were grown on cover slips. In the biofilm phase, both were several-fold more resistant to antibiotics compared to planktonic cells. This is the first study on biofilm forming potential of ocular fluid <i>S. aureus</i> and <i>S. epidermidis</i> on cadaveric cornea, from attachment to dispersal phase of biofilm formation.https://www.mdpi.com/2076-2607/9/6/1124biofilmocular fluidantimicrobial resistanceeye<i>S. aureus</i><i>S. epidermidis</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ranjith Konduri Chinthala Reddy Saiabhilash Sisinthy Shivaji |
spellingShingle |
Ranjith Konduri Chinthala Reddy Saiabhilash Sisinthy Shivaji Biofilm-Forming Potential of Ocular Fluid <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> on Ex Vivo Human Corneas from Attachment to Dispersal Phase Microorganisms biofilm ocular fluid antimicrobial resistance eye <i>S. aureus</i> <i>S. epidermidis</i> |
author_facet |
Ranjith Konduri Chinthala Reddy Saiabhilash Sisinthy Shivaji |
author_sort |
Ranjith Konduri |
title |
Biofilm-Forming Potential of Ocular Fluid <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> on Ex Vivo Human Corneas from Attachment to Dispersal Phase |
title_short |
Biofilm-Forming Potential of Ocular Fluid <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> on Ex Vivo Human Corneas from Attachment to Dispersal Phase |
title_full |
Biofilm-Forming Potential of Ocular Fluid <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> on Ex Vivo Human Corneas from Attachment to Dispersal Phase |
title_fullStr |
Biofilm-Forming Potential of Ocular Fluid <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> on Ex Vivo Human Corneas from Attachment to Dispersal Phase |
title_full_unstemmed |
Biofilm-Forming Potential of Ocular Fluid <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> on Ex Vivo Human Corneas from Attachment to Dispersal Phase |
title_sort |
biofilm-forming potential of ocular fluid <i>staphylococcus aureus</i> and <i>staphylococcus epidermidis</i> on ex vivo human corneas from attachment to dispersal phase |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-05-01 |
description |
The biofilm-forming potential of <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>, isolated from patients with Endophthalmitis, was monitored using glass cover slips and cadaveric corneas as substrata. Both the ocular fluid isolates exhibited biofilm-forming potential by the Congo red agar, Crystal violet and 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H-tetra-zolium hydroxide (XTT) methods. Confocal microscopy demonstrated that the thickness of the biofilm increased from 4–120 h of biofilm formation. Scanning electron microscopic studies indicated that the biofilms grown on cover slips and ex vivo corneas of both the isolates go through an adhesion phase at 4 h followed by multilayer clumping of cells with intercellular connections and copious amounts of extracellular polymeric substance. Clumps subsequently formed columns and eventually single cells were visible indicative of dispersal phase. Biofilm formation was more rapid when the cornea was used as a substratum. In the biofilms grown on corneas, clumping of cells, formation of 3D structures and final appearance of single cells indicative of dispersal phase occurred by 48 h compared to 96–120 h when biofilms were grown on cover slips. In the biofilm phase, both were several-fold more resistant to antibiotics compared to planktonic cells. This is the first study on biofilm forming potential of ocular fluid <i>S. aureus</i> and <i>S. epidermidis</i> on cadaveric cornea, from attachment to dispersal phase of biofilm formation. |
topic |
biofilm ocular fluid antimicrobial resistance eye <i>S. aureus</i> <i>S. epidermidis</i> |
url |
https://www.mdpi.com/2076-2607/9/6/1124 |
work_keys_str_mv |
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