Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour

Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour

The process of alveologenesis is incompletely understood, partly due to the lack of applicable real-time imaging methods. Here the authors describe the process of alveologenesis and the behaviour of epithelial cells in real-time, using widefield microscopy and image deconvolution in precision-cut lu...

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Main Authors: Khondoker M. Akram, Laura L. Yates, Róisín Mongey, Stephen Rothery, David C. A. Gaboriau, Jeremy Sanderson, Matthew Hind, Mark Griffiths, Charlotte H. Dean
Format: Article
Language:English
Published: Nature Publishing Group 2019-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-09067-3
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spelling doaj-24ffe26043ce43fea34a19872584d9052021-05-11T11:41:46ZengNature Publishing GroupNature Communications2041-17232019-03-0110111610.1038/s41467-019-09067-3Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviourKhondoker M. Akram0Laura L. Yates1Róisín Mongey2Stephen Rothery3David C. A. Gaboriau4Jeremy Sanderson5Matthew Hind6Mark Griffiths7Charlotte H. Dean8National Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonFacility for Imaging by Light Microscopy, NHLI, Faculty of Medicine, Imperial College LondonFacility for Imaging by Light Microscopy, NHLI, Faculty of Medicine, Imperial College LondonMRC Harwell Institute, Harwell CampusNational Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonNational Heart and Lung Institute, Imperial College LondonThe process of alveologenesis is incompletely understood, partly due to the lack of applicable real-time imaging methods. Here the authors describe the process of alveologenesis and the behaviour of epithelial cells in real-time, using widefield microscopy and image deconvolution in precision-cut lung slices, revealing the dominant role of epithelial cell migration.https://doi.org/10.1038/s41467-019-09067-3
collection DOAJ
language English
format Article
sources DOAJ
author Khondoker M. Akram
Laura L. Yates
Róisín Mongey
Stephen Rothery
David C. A. Gaboriau
Jeremy Sanderson
Matthew Hind
Mark Griffiths
Charlotte H. Dean
spellingShingle Khondoker M. Akram
Laura L. Yates
Róisín Mongey
Stephen Rothery
David C. A. Gaboriau
Jeremy Sanderson
Matthew Hind
Mark Griffiths
Charlotte H. Dean
Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour
Nature Communications
author_facet Khondoker M. Akram
Laura L. Yates
Róisín Mongey
Stephen Rothery
David C. A. Gaboriau
Jeremy Sanderson
Matthew Hind
Mark Griffiths
Charlotte H. Dean
author_sort Khondoker M. Akram
title Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour
title_short Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour
title_full Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour
title_fullStr Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour
title_full_unstemmed Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour
title_sort live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-03-01
description The process of alveologenesis is incompletely understood, partly due to the lack of applicable real-time imaging methods. Here the authors describe the process of alveologenesis and the behaviour of epithelial cells in real-time, using widefield microscopy and image deconvolution in precision-cut lung slices, revealing the dominant role of epithelial cell migration.
url https://doi.org/10.1038/s41467-019-09067-3
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