Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortex

Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortex

We studied the effect of kindling, a model of temporal lobe epilepsy, on the frequency-dependent information transfer from the entorhinal cortex to the hippocampus in vitro. In control rats repetitive synaptic activation of layer III projection cells resulted in a frequency dependent depression of t...

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Main Authors: Tengis Gloveli, Joachim Behr, Tamar Dugladze, Zaal Kokaia, Merab Kokaia, Uwe Heinemann
Format: Article
Language:English
Published: Elsevier 2003-08-01
Series:Neurobiology of Disease
Subjects:
Projection cells
Intracellular recording
Kindling
GABAB
Synaptic transmission
In vitro
Online Access:http://www.sciencedirect.com/science/article/pii/S0969996103000391
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spelling doaj-250f3622aef84873b824bcf4130cf1cd2021-03-20T04:48:26ZengElsevierNeurobiology of Disease1095-953X2003-08-01133203212Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortexTengis Gloveli0Joachim Behr1Tamar Dugladze2Zaal Kokaia3Merab Kokaia4Uwe Heinemann5Johannes-Müller-Institute of Physiology at the Charité, Humboldt University Berlin, Tucholskystr. 2, 10117 Berlin, Germany; Neuroscience Research Center at the Charité, Humboldt University Berlin, Schumannstr. 20/21, 10117 Berlin, Germany; Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, BMC A-11, 22184, Lund, SwedenJohannes-Müller-Institute of Physiology at the Charité, Humboldt University Berlin, Tucholskystr. 2, 10117 Berlin, Germany; Neuroscience Research Center at the Charité, Humboldt University Berlin, Schumannstr. 20/21, 10117 Berlin, Germany; Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, BMC A-11, 22184, Lund, SwedenJohannes-Müller-Institute of Physiology at the Charité, Humboldt University Berlin, Tucholskystr. 2, 10117 Berlin, Germany; Neuroscience Research Center at the Charité, Humboldt University Berlin, Schumannstr. 20/21, 10117 Berlin, Germany; Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, BMC A-11, 22184, Lund, SwedenJohannes-Müller-Institute of Physiology at the Charité, Humboldt University Berlin, Tucholskystr. 2, 10117 Berlin, Germany; Neuroscience Research Center at the Charité, Humboldt University Berlin, Schumannstr. 20/21, 10117 Berlin, Germany; Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, BMC A-11, 22184, Lund, SwedenJohannes-Müller-Institute of Physiology at the Charité, Humboldt University Berlin, Tucholskystr. 2, 10117 Berlin, Germany; Neuroscience Research Center at the Charité, Humboldt University Berlin, Schumannstr. 20/21, 10117 Berlin, Germany; Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, BMC A-11, 22184, Lund, SwedenJohannes-Müller-Institute of Physiology at the Charité, Humboldt University Berlin, Tucholskystr. 2, 10117 Berlin, Germany; Neuroscience Research Center at the Charité, Humboldt University Berlin, Schumannstr. 20/21, 10117 Berlin, Germany; Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, BMC A-11, 22184, Lund, SwedenWe studied the effect of kindling, a model of temporal lobe epilepsy, on the frequency-dependent information transfer from the entorhinal cortex to the hippocampus in vitro. In control rats repetitive synaptic activation of layer III projection cells resulted in a frequency dependent depression of the synaptic transfer of action potentials to the hippocampus. One-to-two-days after kindling this effect was strongly reduced. Although no substantial change in synaptic inhibition upon single electrical stimulation was detected in kindled rats, there was a significant depression in the prolonged inhibition following high frequency stimulation. In kindled animals, paired-pulse depression (PPD) of stimulus-evoked IPSCs in layer III neurons was significantly stronger than in control rats. The increase of PPD is most likely caused by an increased presynaptic GABAB receptor-mediated autoinhibition. In kindled animals activation of presynaptic GABAB receptors by baclofen (10 μM) suppressed monosynaptic IPSCs significantly more than in control rats. In contrast, activation of postsynaptic GABAB receptors by baclofen was accompanied by comparable changes of the membrane conductance in both animal groups. Thus, in kindled animals activation of the layer III-CA1 pathway is facilitated by an increased GABAB receptor-mediated autoinhibition leading to an enhanced activation of the monosynaptic EC-CA1 pathway.http://www.sciencedirect.com/science/article/pii/S0969996103000391Projection cellsIntracellular recordingKindlingGABABSynaptic transmissionIn vitro
collection DOAJ
language English
format Article
sources DOAJ
author Tengis Gloveli
Joachim Behr
Tamar Dugladze
Zaal Kokaia
Merab Kokaia
Uwe Heinemann
spellingShingle Tengis Gloveli
Joachim Behr
Tamar Dugladze
Zaal Kokaia
Merab Kokaia
Uwe Heinemann
Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortex
Neurobiology of Disease
Projection cells
Intracellular recording
Kindling
GABAB
Synaptic transmission
In vitro
author_facet Tengis Gloveli
Joachim Behr
Tamar Dugladze
Zaal Kokaia
Merab Kokaia
Uwe Heinemann
author_sort Tengis Gloveli
title Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortex
title_short Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortex
title_full Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortex
title_fullStr Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortex
title_full_unstemmed Kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic GABAb autoreceptors in layer III of the entorhinal cortex
title_sort kindling alters entorhinal cortex-hippocampal interaction by increased efficacy of presynaptic gabab autoreceptors in layer iii of the entorhinal cortex
publisher Elsevier
series Neurobiology of Disease
issn 1095-953X
publishDate 2003-08-01
description We studied the effect of kindling, a model of temporal lobe epilepsy, on the frequency-dependent information transfer from the entorhinal cortex to the hippocampus in vitro. In control rats repetitive synaptic activation of layer III projection cells resulted in a frequency dependent depression of the synaptic transfer of action potentials to the hippocampus. One-to-two-days after kindling this effect was strongly reduced. Although no substantial change in synaptic inhibition upon single electrical stimulation was detected in kindled rats, there was a significant depression in the prolonged inhibition following high frequency stimulation. In kindled animals, paired-pulse depression (PPD) of stimulus-evoked IPSCs in layer III neurons was significantly stronger than in control rats. The increase of PPD is most likely caused by an increased presynaptic GABAB receptor-mediated autoinhibition. In kindled animals activation of presynaptic GABAB receptors by baclofen (10 μM) suppressed monosynaptic IPSCs significantly more than in control rats. In contrast, activation of postsynaptic GABAB receptors by baclofen was accompanied by comparable changes of the membrane conductance in both animal groups. Thus, in kindled animals activation of the layer III-CA1 pathway is facilitated by an increased GABAB receptor-mediated autoinhibition leading to an enhanced activation of the monosynaptic EC-CA1 pathway.
topic Projection cells
Intracellular recording
Kindling
GABAB
Synaptic transmission
In vitro
url http://www.sciencedirect.com/science/article/pii/S0969996103000391
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