Optimization of glycine and thiotriazoline compound analysis by high-performance liquid chromatography

• Main Authors: L. I. Kucherenko, O. V. Khromylova, O. O. Portna, H. I. Tkachenko
• Format: Article
• Language: English
• Published: Zaporozhye State Medical University 2020-08-01
• Series: Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki
• Subjects: glycine; thiotriazoline; tablets; amino acids; hplc
• Online Access: http://pharmed.zsmu.edu.ua/article/view/207180/207482

Today, Ukraine is ranked first in Europe in terms of cerebrovascular morbidity and mortality from stroke. Therefore, the creation of new drugs for the treatment of these pathologies is an urgent task of modern pharmacy. For that reason, the creation of a new combination drug based on glycine – a neurotransmitter amino acid and thiothiazolin – an antioxidant is appropriate and relevant. For the new combination drug, a rational dosage form in the form of tablets was proposed. New standardization methods should be developed for the combination of tablets. Nowadays, physical-chemical methods of research are widely used for standardization of finished dosage forms, both in pharmacy and factory production. Our attention was drawn to the method of high-performance liquid chromatography, which allows us to simultaneously standardize the active substances in one sample. To develop the HPLC methodology, it is first necessary to select the optimal conditions for the analysis of active substances. The purpose of work is a selection of optimal conditions for the simultaneous determination of glycine with thiotriazoline in the model mixture by HPLC method. Materials and methods. In the course of the research, glycine, thiotriazolin were used. The studies were performed using a BISCHOFF HPLC modular system with a Lambda 1010 spectrophotometric detector. The columns used were: Prontosil 120-5-CN, Hypersil ODS-C18-5u. As eluent used: water; 0.05 % aqueous trifluoroacetic acid solution; Bu4NHSO4 3.4 g/l in water; Bu4NHSO4 3.4 g/l, 0.02 M Na2HPO4 in water; Bu4NHSO4 3.4 g/l, 0.05 % trifluoroacetic acid solution in water. Results. As can be seen from the results obtained, it is expedient to use tetrabutylammonium in the conditions of pair chromatography on the reverse phase, among different eluents and phases used, for the subsequent determination of glycine with thiotriazolin, both in the model mixture and in the combined dosage forms with the simultaneous use of acid buffer – 0.05 % solution of trifluoroacetic acid. Conclusions. During the research, the optimal conditions for the simultaneous determination of glycine with thiotriazoline in one sample by HPLC were selected. Іt was found that the determination of the active substances should be carried in the conditions of pair chromatography on the reverse phase when using tetrabutylammonium eluent with the simultaneous use of acid buffer – 0.05 % solution of trifluoroacetic acid