On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity
Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV l...
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doaj-2535bb1b946141108c4ea6153d6bbdc62020-11-24T22:09:52ZengMDPI AGMolecules1420-30492018-03-0123479610.3390/molecules23040796molecules23040796On-Demand Bioadhesive Dendrimers with Reduced CytotoxicityFeng Gao0Ivan Djordjevic1Oleksandr Pokholenko2Haobo Zhang3Junying Zhang4Terry W. J. Steele5School of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, ChinaEscuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, MexicoSchool of Materials Science and Engineering, Division of Materials Technology, Nanyang Technological University, Singapore 639798, SingaporeSchool of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, ChinaSchool of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, ChinaSchool of Materials Science and Engineering, Division of Materials Technology, Nanyang Technological University, Singapore 639798, SingaporeTissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic-co-glycolic acid) (PLGA) thin films.http://www.mdpi.com/1420-3049/23/4/796PAMAMdendrimersbioadhesivediazirine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng Gao Ivan Djordjevic Oleksandr Pokholenko Haobo Zhang Junying Zhang Terry W. J. Steele |
spellingShingle |
Feng Gao Ivan Djordjevic Oleksandr Pokholenko Haobo Zhang Junying Zhang Terry W. J. Steele On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity Molecules PAMAM dendrimers bioadhesive diazirine |
author_facet |
Feng Gao Ivan Djordjevic Oleksandr Pokholenko Haobo Zhang Junying Zhang Terry W. J. Steele |
author_sort |
Feng Gao |
title |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_short |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_full |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_fullStr |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_full_unstemmed |
On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity |
title_sort |
on-demand bioadhesive dendrimers with reduced cytotoxicity |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-03-01 |
description |
Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic-co-glycolic acid) (PLGA) thin films. |
topic |
PAMAM dendrimers bioadhesive diazirine |
url |
http://www.mdpi.com/1420-3049/23/4/796 |
work_keys_str_mv |
AT fenggao ondemandbioadhesivedendrimerswithreducedcytotoxicity AT ivandjordjevic ondemandbioadhesivedendrimerswithreducedcytotoxicity AT oleksandrpokholenko ondemandbioadhesivedendrimerswithreducedcytotoxicity AT haobozhang ondemandbioadhesivedendrimerswithreducedcytotoxicity AT junyingzhang ondemandbioadhesivedendrimerswithreducedcytotoxicity AT terrywjsteele ondemandbioadhesivedendrimerswithreducedcytotoxicity |
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1725810369963753472 |