On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV l...

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Main Authors:	Feng Gao, Ivan Djordjevic, Oleksandr Pokholenko, Haobo Zhang, Junying Zhang, Terry W. J. Steele
Format:	Article
Language:	English
Published:	MDPI AG 2018-03-01
Series:	Molecules
Subjects:	PAMAM dendrimers bioadhesive diazirine
Online Access:	http://www.mdpi.com/1420-3049/23/4/796

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spelling	doaj-2535bb1b946141108c4ea6153d6bbdc62020-11-24T22:09:52ZengMDPI AGMolecules1420-30492018-03-0123479610.3390/molecules23040796molecules23040796On-Demand Bioadhesive Dendrimers with Reduced CytotoxicityFeng Gao0Ivan Djordjevic1Oleksandr Pokholenko2Haobo Zhang3Junying Zhang4Terry W. J. Steele5School of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, ChinaEscuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, MexicoSchool of Materials Science and Engineering, Division of Materials Technology, Nanyang Technological University, Singapore 639798, SingaporeSchool of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, ChinaSchool of Material Science and Engineering, Beijing University of Chemistry Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, ChinaSchool of Materials Science and Engineering, Division of Materials Technology, Nanyang Technological University, Singapore 639798, SingaporeTissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic-co-glycolic acid) (PLGA) thin films.http://www.mdpi.com/1420-3049/23/4/796PAMAMdendrimersbioadhesivediazirine
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Feng Gao Ivan Djordjevic Oleksandr Pokholenko Haobo Zhang Junying Zhang Terry W. J. Steele
spellingShingle	Feng Gao Ivan Djordjevic Oleksandr Pokholenko Haobo Zhang Junying Zhang Terry W. J. Steele On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity Molecules PAMAM dendrimers bioadhesive diazirine
author_facet	Feng Gao Ivan Djordjevic Oleksandr Pokholenko Haobo Zhang Junying Zhang Terry W. J. Steele
author_sort	Feng Gao
title	On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity
title_short	On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity
title_full	On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity
title_fullStr	On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity
title_full_unstemmed	On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity
title_sort	on-demand bioadhesive dendrimers with reduced cytotoxicity
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2018-03-01
description	Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic-co-glycolic acid) (PLGA) thin films.
topic	PAMAM dendrimers bioadhesive diazirine
url	http://www.mdpi.com/1420-3049/23/4/796
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On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

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