Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis
Norovirus is a major pathogen identified in children with acute gastroenteritis (AGE), little is known about the strain’s diversity and their clinical severity. Stool and/or rectal swabs were collected from children ≤18 years of age recruited at emergency departments (ED), and a provincial nursing a...
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doaj-254769a2f1c5444799962730697ad3752020-11-27T08:12:27ZengMDPI AGMicroorganisms2076-26072020-11-0181873187310.3390/microorganisms8121873Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute GastroenteritisSudha Bhavanam0Stephen B. Freedman1Bonita E. Lee2Ran Zhuo3Yuanyuan Qiu4Linda Chui5Jianling Xie6Samina Ali7Otto G. Vanderkooi8Xiaoli L. Pang9on behalf of the Alberta Provincial Pediatric Enteric Infection Team (APPETITE)10Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaDivisions of Pediatric Emergency Medicine and Gastroenterology, Departments of Pediatrics and Emergency Medicine, Alberta Children’s Hospital, Alberta Children’s Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, AB T3B 6A8, CanadaDepartment of Pediatrics, Faculty of Medicine & Dentistry, Women and Children’s Health Research Institute, Stollery Children’s Hospital, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartments of Pediatrics, Cumming School of Medicine, University of Calgary, Calgary, AB T3B 6A8, CanadaDepartment of Pediatrics, Faculty of Medicine & Dentistry, Women and Children’s Health Research Institute, Stollery Children’s Hospital, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartments of Pediatrics, Microbiology, Immunology and Infectious Diseases, Pathology & Laboratory Medicine and Community Health Sciences, Alberta Children’s Hospital, Alberta Children’s Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, AB T3B 6A8, CanadaDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, CanadaUniversity of Alberta, Edmonton, AB T6G 2R3, CanadaNorovirus is a major pathogen identified in children with acute gastroenteritis (AGE), little is known about the strain’s diversity and their clinical severity. Stool and/or rectal swabs were collected from children ≤18 years of age recruited at emergency departments (ED), and a provincial nursing advice phone line due to AGE symptoms in the province of Alberta, Canada between December 2014 and August 2018. Specimens were tested using a reverse transcription real time PCR and genotyped by Sanger sequencing. The Modified Vesikari Scale score (MVS) was used to evaluate the disease severity. The objectives are to identify the Genogroup and Genotype distribution and to compare illness severity between the GI and GII genogroups and to complete further analyses comparing the GII genotypes identified. GII.4 was the genotype most commonly identified. Children with GII.4 had higher MVS scores (12.0 (10.0, 14.0; <i>p</i> = 0.002)) and more prolonged diarrheal (5 days (3.0, 7.8)) and vomiting (3.2 days (1.7, 5.3; <i>p </i>< 0.001)) durations compared to other non GII.4 strains. The predominant strain varied by year with GII.4 Sydney[P31] predominant in 2014/15, GII.4 Sydney[P16] in 2015/16 and 2017/18, and GII.3[P12] in 2016/17. Genogroup II norovirus strains predominated in children with AGE with variance between years; clinical severity associated with different strains varied with episodes being most severe among GII.4 infected children.https://www.mdpi.com/2076-2607/8/12/1873norovirusgastroenteritisclinical severitygenotypeschildren |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sudha Bhavanam Stephen B. Freedman Bonita E. Lee Ran Zhuo Yuanyuan Qiu Linda Chui Jianling Xie Samina Ali Otto G. Vanderkooi Xiaoli L. Pang on behalf of the Alberta Provincial Pediatric Enteric Infection Team (APPETITE) |
spellingShingle |
Sudha Bhavanam Stephen B. Freedman Bonita E. Lee Ran Zhuo Yuanyuan Qiu Linda Chui Jianling Xie Samina Ali Otto G. Vanderkooi Xiaoli L. Pang on behalf of the Alberta Provincial Pediatric Enteric Infection Team (APPETITE) Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis Microorganisms norovirus gastroenteritis clinical severity genotypes children |
author_facet |
Sudha Bhavanam Stephen B. Freedman Bonita E. Lee Ran Zhuo Yuanyuan Qiu Linda Chui Jianling Xie Samina Ali Otto G. Vanderkooi Xiaoli L. Pang on behalf of the Alberta Provincial Pediatric Enteric Infection Team (APPETITE) |
author_sort |
Sudha Bhavanam |
title |
Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis |
title_short |
Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis |
title_full |
Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis |
title_fullStr |
Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis |
title_full_unstemmed |
Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis |
title_sort |
differences in illness severity among circulating norovirus genotypes in a large pediatric cohort with acute gastroenteritis |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-11-01 |
description |
Norovirus is a major pathogen identified in children with acute gastroenteritis (AGE), little is known about the strain’s diversity and their clinical severity. Stool and/or rectal swabs were collected from children ≤18 years of age recruited at emergency departments (ED), and a provincial nursing advice phone line due to AGE symptoms in the province of Alberta, Canada between December 2014 and August 2018. Specimens were tested using a reverse transcription real time PCR and genotyped by Sanger sequencing. The Modified Vesikari Scale score (MVS) was used to evaluate the disease severity. The objectives are to identify the Genogroup and Genotype distribution and to compare illness severity between the GI and GII genogroups and to complete further analyses comparing the GII genotypes identified. GII.4 was the genotype most commonly identified. Children with GII.4 had higher MVS scores (12.0 (10.0, 14.0; <i>p</i> = 0.002)) and more prolonged diarrheal (5 days (3.0, 7.8)) and vomiting (3.2 days (1.7, 5.3; <i>p </i>< 0.001)) durations compared to other non GII.4 strains. The predominant strain varied by year with GII.4 Sydney[P31] predominant in 2014/15, GII.4 Sydney[P16] in 2015/16 and 2017/18, and GII.3[P12] in 2016/17. Genogroup II norovirus strains predominated in children with AGE with variance between years; clinical severity associated with different strains varied with episodes being most severe among GII.4 infected children. |
topic |
norovirus gastroenteritis clinical severity genotypes children |
url |
https://www.mdpi.com/2076-2607/8/12/1873 |
work_keys_str_mv |
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