Detection and Genotyping of Epstein-Bar Virus Among Paraffin Embedded Tissues of Hodgkin and Non-Hodgkin's Lymphoma Patients in Ahvaz, Iran

• Main Authors: Ala Habibian, Manoochehr Makvandi, Alireza Samarbaf-Zadeh, Niloofar Neisi, Rahim Soleimani-Jelodar, Kamyar Makvandi, Shima Izadi
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2018-08-01
• Series: Acta Medica Iranica
• Subjects: EBNA-1; EBNA-3C; Epstein-Barr virus; Hodgkin lymphoma; Non-hodgkin lymphoma
• Online Access: https://acta.tums.ac.ir/index.php/acta/article/view/6605

Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infected with 90% of the world’s population. EBV is transmitted through saliva and associated with different diseases such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitt`s lymphoma, Hodgkin and Non-Hodgkin`s lymphoma. This study was designed to determine the frequency of EBV DNA and genotyping of this virus in histological tissues of Hodgkin and Non-Hodgkin's lymphoma in Ahvaz, Iran. In this study, 12 samples of Hodgkin and 29 samples of Non-Hodgkin`s lymphoma were examined from Ahvaz Imam Khomeini Hospital. After deparaffinization and DNA extraction, Nested-PCR technique was carried out on Epstein-Barr nuclear antigen 1 (EBNA-1) and Epstein-Barr nuclear antigen 3C (EBNA-3C) regions in order to detect EBV genome and genotype in tumoral tissues. Among 12 cases of Hodgkin`s lymphoma, 3 (25%) cases and among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive for EBV. All 3 EBV positive cases of Hodgkin`s lymphoma and a number of 8 (57%) cases of Non-Hodgkin`s lymphoma belonged to the adolescence age group which proves an association between age and EBV positive in Hodgkin and Non-Hodgkin`s lymphoma according to Fisher`s exact test (P=0.03). All EBV positive cases in Hodgkin and Non-Hodgkin`s lymphoma were EBV-1. The results of this study revealed that EBV played a possible important role in Hodgkin and Non-Hodgkin`s lymphoma especially in adults. Also, the main genotype of virus in the current study was EBV-1.