Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis
Rheumatoid arthritis (RA) is the most common autoimmune inflammatory disease, and single periodontitis-associated bacteria have been suggested in disease manifestation. Here, the oral microbiota was characterized in relation to the early onset of RA (eRA) taking periodontal status into consideration...
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doaj-2560441f39624922a0d96f1beee245712021-08-26T14:05:43ZengMDPI AGMicroorganisms2076-26072021-08-0191657165710.3390/microorganisms9081657Oral Microbiota Identifies Patients in Early Onset Rheumatoid ArthritisAnders Esberg0Linda Johansson1Ingegerd Johansson2Solbritt Rantapää Dahlqvist3Department of Odontology, Umeå University, 901 87 Umeå, SwedenDepartment of Public Health and Medicine, Rheumatology, Umeå University, 901 87 Umeå, SwedenDepartment of Odontology, Umeå University, 901 87 Umeå, SwedenDepartment of Public Health and Medicine, Rheumatology, Umeå University, 901 87 Umeå, SwedenRheumatoid arthritis (RA) is the most common autoimmune inflammatory disease, and single periodontitis-associated bacteria have been suggested in disease manifestation. Here, the oral microbiota was characterized in relation to the early onset of RA (eRA) taking periodontal status into consideration. 16S rRNA gene amplicon sequencing of saliva bacterial DNA from 61 eRA patients without disease-modifying anti-rheumatic drugs and 59 matched controls was performed. Taxonomic classification at 98.5% was conducted against the Human Oral Microbiome Database, microbiota functions were predicted using PICRUSt, and periodontal status linked from the Swedish quality register for clinically assessed caries and periodontitis. The participants were classified into three distinct microbiota-based cluster groups with cluster allocation differences by eRA status. Independently of periodontal status, eRA patients had enriched levels of <i>Prevotella pleuritidis</i>, <i>Treponema denticola</i>, <i>Porphyromonas endodontalis</i> and <i>Filifactor alocis</i> species and in the <i>Porphyromonas</i> and <i>Fusobacterium</i> genera and functions linked to ornithine metabolism, glucosylceramidase, beta-lactamase resistance, biphenyl degradation, fatty acid metabolism and 17-beta-estradiol-17-dehydrogenase metabolism. The results support a deviating oral microbiota composition already in eRA patients compared with healthy controls and highlight a panel of oral bacteria that may be useful in eRA risk assessment in both periodontally healthy and diseased persons.https://www.mdpi.com/2076-2607/9/8/1657rheumatoid arthritisoral microbiotaperiodontitis16S rDNA sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anders Esberg Linda Johansson Ingegerd Johansson Solbritt Rantapää Dahlqvist |
spellingShingle |
Anders Esberg Linda Johansson Ingegerd Johansson Solbritt Rantapää Dahlqvist Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis Microorganisms rheumatoid arthritis oral microbiota periodontitis 16S rDNA sequencing |
author_facet |
Anders Esberg Linda Johansson Ingegerd Johansson Solbritt Rantapää Dahlqvist |
author_sort |
Anders Esberg |
title |
Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis |
title_short |
Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis |
title_full |
Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis |
title_fullStr |
Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis |
title_full_unstemmed |
Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis |
title_sort |
oral microbiota identifies patients in early onset rheumatoid arthritis |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-08-01 |
description |
Rheumatoid arthritis (RA) is the most common autoimmune inflammatory disease, and single periodontitis-associated bacteria have been suggested in disease manifestation. Here, the oral microbiota was characterized in relation to the early onset of RA (eRA) taking periodontal status into consideration. 16S rRNA gene amplicon sequencing of saliva bacterial DNA from 61 eRA patients without disease-modifying anti-rheumatic drugs and 59 matched controls was performed. Taxonomic classification at 98.5% was conducted against the Human Oral Microbiome Database, microbiota functions were predicted using PICRUSt, and periodontal status linked from the Swedish quality register for clinically assessed caries and periodontitis. The participants were classified into three distinct microbiota-based cluster groups with cluster allocation differences by eRA status. Independently of periodontal status, eRA patients had enriched levels of <i>Prevotella pleuritidis</i>, <i>Treponema denticola</i>, <i>Porphyromonas endodontalis</i> and <i>Filifactor alocis</i> species and in the <i>Porphyromonas</i> and <i>Fusobacterium</i> genera and functions linked to ornithine metabolism, glucosylceramidase, beta-lactamase resistance, biphenyl degradation, fatty acid metabolism and 17-beta-estradiol-17-dehydrogenase metabolism. The results support a deviating oral microbiota composition already in eRA patients compared with healthy controls and highlight a panel of oral bacteria that may be useful in eRA risk assessment in both periodontally healthy and diseased persons. |
topic |
rheumatoid arthritis oral microbiota periodontitis 16S rDNA sequencing |
url |
https://www.mdpi.com/2076-2607/9/8/1657 |
work_keys_str_mv |
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