Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and Invasion

Background and objective To detect the expression of neutrophil gelatinase-assoeiated lipocalin (NGAL) in the different differentiations of lung cancer tissues and to study the mechanism of invasion of A549 cells affected by NGAL. Methods The expression of NGAL was detected by immunochemistry in lun...

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Main Authors: Jian TANG, Jie LI, Shaojun LI, Jingbo LI, Changhai YU, Chengze WEI
Format: Article
Language:zho
Published: Chinese Anti-Cancer Association; Chinese Antituberculosis Association 2015-04-01
Series:Chinese Journal of Lung Cancer
Subjects:
Online Access:http://dx.doi.org/10.3779/j.issn.1009-3419.2015.04.03
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spelling doaj-25647a13dd44404e80f125dd40eb42282020-11-24T23:08:21ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872015-04-0118418719210.3779/j.issn.1009-3419.2015.04.03Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and InvasionJian TANG0Jie LI1Shaojun LI2Jingbo LI3Changhai YU4Chengze WEI5Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, ChinaDepartment of Chest Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, ChinaDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100048, ChinaDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100048, ChinaDepartment of Cardiothoracic Surgery, the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing 100048, ChinaAffiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, ChinaBackground and objective To detect the expression of neutrophil gelatinase-assoeiated lipocalin (NGAL) in the different differentiations of lung cancer tissues and to study the mechanism of invasion of A549 cells affected by NGAL. Methods The expression of NGAL was detected by immunochemistry in lung cancer tissue and the tissue around edge of the cancer. The effect of NGAL expression on A549 cells was observed by using qRT-PCR and Western blot. The abilities of invasion and metastasis were evaluated by transwell invasion and migration assay, and cell scratch assay in vitro. The protein expression of E-cadherin, Vimentin was measured by immunofluoresence and Western blot. Results The positive expression rate of NGAL was 76.32% (58/76) in the lung cancer, 13.3% (4/30) in adjacent tissue by immunochemistry. NGAL expression levels in the lung cancer tissues were significantly higher than that in adjacent tissues. The rate of migration and invasion in NGAL-siRNA group was 60.4%±6.4% compared to 50.5%±4.4% in the control group, there was a significant difference (P<0.05). Vimentin was suppressed, and E-cadherin was upregulated when NGAL was inhibited. MMP-2 and MMP-9 decreased when NGAL was knocked down. Conclusion The expression level of NGAL is highly expressed in lung cancer. NGAL may be one of important indicators involved in lung cancer infiltrated and transferred. NGAL might be one of potential targets for lung cancer treatment.http://dx.doi.org/10.3779/j.issn.1009-3419.2015.04.03Lung neoplasmsNeutrophil gelatinase-associated lipocalinMigrationInvasionEpithelial-mesenchymal transition
collection DOAJ
language zho
format Article
sources DOAJ
author Jian TANG
Jie LI
Shaojun LI
Jingbo LI
Changhai YU
Chengze WEI
spellingShingle Jian TANG
Jie LI
Shaojun LI
Jingbo LI
Changhai YU
Chengze WEI
Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and Invasion
Chinese Journal of Lung Cancer
Lung neoplasms
Neutrophil gelatinase-associated lipocalin
Migration
Invasion
Epithelial-mesenchymal transition
author_facet Jian TANG
Jie LI
Shaojun LI
Jingbo LI
Changhai YU
Chengze WEI
author_sort Jian TANG
title Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and Invasion
title_short Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and Invasion
title_full Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and Invasion
title_fullStr Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and Invasion
title_full_unstemmed Effect of Inhibiting NGAL Gene Expression on A549 Lung Cancer Cell Migration and Invasion
title_sort effect of inhibiting ngal gene expression on a549 lung cancer cell migration and invasion
publisher Chinese Anti-Cancer Association; Chinese Antituberculosis Association
series Chinese Journal of Lung Cancer
issn 1009-3419
1999-6187
publishDate 2015-04-01
description Background and objective To detect the expression of neutrophil gelatinase-assoeiated lipocalin (NGAL) in the different differentiations of lung cancer tissues and to study the mechanism of invasion of A549 cells affected by NGAL. Methods The expression of NGAL was detected by immunochemistry in lung cancer tissue and the tissue around edge of the cancer. The effect of NGAL expression on A549 cells was observed by using qRT-PCR and Western blot. The abilities of invasion and metastasis were evaluated by transwell invasion and migration assay, and cell scratch assay in vitro. The protein expression of E-cadherin, Vimentin was measured by immunofluoresence and Western blot. Results The positive expression rate of NGAL was 76.32% (58/76) in the lung cancer, 13.3% (4/30) in adjacent tissue by immunochemistry. NGAL expression levels in the lung cancer tissues were significantly higher than that in adjacent tissues. The rate of migration and invasion in NGAL-siRNA group was 60.4%±6.4% compared to 50.5%±4.4% in the control group, there was a significant difference (P<0.05). Vimentin was suppressed, and E-cadherin was upregulated when NGAL was inhibited. MMP-2 and MMP-9 decreased when NGAL was knocked down. Conclusion The expression level of NGAL is highly expressed in lung cancer. NGAL may be one of important indicators involved in lung cancer infiltrated and transferred. NGAL might be one of potential targets for lung cancer treatment.
topic Lung neoplasms
Neutrophil gelatinase-associated lipocalin
Migration
Invasion
Epithelial-mesenchymal transition
url http://dx.doi.org/10.3779/j.issn.1009-3419.2015.04.03
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