Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains.
Escherichia coli represents a classical intestinal gram-negative commensal. Despite this commensalism, different E. coli strains can mediate disparate immunogenic properties in a given host. Symbiotic E. coli strains such as E. coli Nissle 1917 (EcN) are attributed beneficial properties, e.g., promo...
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doaj-25a28d77ce84464a8ca7a455fcb19ac42021-07-02T17:19:53ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852019-06-01176e300033410.1371/journal.pbio.3000334Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains.Alex SteimleSarah MenzAnnika BenderBrianna BallAlexander N R WeberThomas HagemannAnna LangeJan K MaerzRaphael ParuselLena MichaelisAndrea SchäferHans YaoHanna-Christine LöwSina BeierMehari Tesfazgi MebrhatuKerstin GronbachSamuel WagnerDavid VoehringerMartin SchallerBirgit FehrenbacherIngo B AutenriethTobias A OelschlaegerJulia-Stefanie FrickEscherichia coli represents a classical intestinal gram-negative commensal. Despite this commensalism, different E. coli strains can mediate disparate immunogenic properties in a given host. Symbiotic E. coli strains such as E. coli Nissle 1917 (EcN) are attributed beneficial properties, e.g., promotion of intestinal homeostasis. Therefore, we aimed to identify molecular features derived from symbiotic bacteria that might help to develop innovative therapeutic alternatives for the treatment of intestinal immune disorders. This study was performed using the dextran sodium sulphate (DSS)-induced colitis mouse model, which is routinely used to evaluate potential therapeutics for the treatment of Inflammatory Bowel Diseases (IBDs). We focused on the analysis of flagellin structures of different E. coli strains. EcN flagellin was found to harbor a substantially longer hypervariable region (HVR) compared to other commensal E. coli strains, and this longer HVR mediated symbiotic properties through stronger activation of Toll-like receptor (TLR)5, thereby resulting in interleukin (IL)-22-mediated protection of mice against DSS-induced colitis. Furthermore, using bone-marrow-chimeric mice (BMCM), CD11c+ cells of the colonic lamina propria (LP) were identified as the main mediators of these flagellin-induced symbiotic effects. We propose flagellin from symbiotic E. coli strains as a potential therapeutic to restore intestinal immune homeostasis, e.g., for the treatment of IBD patients.https://doi.org/10.1371/journal.pbio.3000334 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alex Steimle Sarah Menz Annika Bender Brianna Ball Alexander N R Weber Thomas Hagemann Anna Lange Jan K Maerz Raphael Parusel Lena Michaelis Andrea Schäfer Hans Yao Hanna-Christine Löw Sina Beier Mehari Tesfazgi Mebrhatu Kerstin Gronbach Samuel Wagner David Voehringer Martin Schaller Birgit Fehrenbacher Ingo B Autenrieth Tobias A Oelschlaeger Julia-Stefanie Frick |
spellingShingle |
Alex Steimle Sarah Menz Annika Bender Brianna Ball Alexander N R Weber Thomas Hagemann Anna Lange Jan K Maerz Raphael Parusel Lena Michaelis Andrea Schäfer Hans Yao Hanna-Christine Löw Sina Beier Mehari Tesfazgi Mebrhatu Kerstin Gronbach Samuel Wagner David Voehringer Martin Schaller Birgit Fehrenbacher Ingo B Autenrieth Tobias A Oelschlaeger Julia-Stefanie Frick Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains. PLoS Biology |
author_facet |
Alex Steimle Sarah Menz Annika Bender Brianna Ball Alexander N R Weber Thomas Hagemann Anna Lange Jan K Maerz Raphael Parusel Lena Michaelis Andrea Schäfer Hans Yao Hanna-Christine Löw Sina Beier Mehari Tesfazgi Mebrhatu Kerstin Gronbach Samuel Wagner David Voehringer Martin Schaller Birgit Fehrenbacher Ingo B Autenrieth Tobias A Oelschlaeger Julia-Stefanie Frick |
author_sort |
Alex Steimle |
title |
Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains. |
title_short |
Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains. |
title_full |
Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains. |
title_fullStr |
Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains. |
title_full_unstemmed |
Flagellin hypervariable region determines symbiotic properties of commensal Escherichia coli strains. |
title_sort |
flagellin hypervariable region determines symbiotic properties of commensal escherichia coli strains. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Biology |
issn |
1544-9173 1545-7885 |
publishDate |
2019-06-01 |
description |
Escherichia coli represents a classical intestinal gram-negative commensal. Despite this commensalism, different E. coli strains can mediate disparate immunogenic properties in a given host. Symbiotic E. coli strains such as E. coli Nissle 1917 (EcN) are attributed beneficial properties, e.g., promotion of intestinal homeostasis. Therefore, we aimed to identify molecular features derived from symbiotic bacteria that might help to develop innovative therapeutic alternatives for the treatment of intestinal immune disorders. This study was performed using the dextran sodium sulphate (DSS)-induced colitis mouse model, which is routinely used to evaluate potential therapeutics for the treatment of Inflammatory Bowel Diseases (IBDs). We focused on the analysis of flagellin structures of different E. coli strains. EcN flagellin was found to harbor a substantially longer hypervariable region (HVR) compared to other commensal E. coli strains, and this longer HVR mediated symbiotic properties through stronger activation of Toll-like receptor (TLR)5, thereby resulting in interleukin (IL)-22-mediated protection of mice against DSS-induced colitis. Furthermore, using bone-marrow-chimeric mice (BMCM), CD11c+ cells of the colonic lamina propria (LP) were identified as the main mediators of these flagellin-induced symbiotic effects. We propose flagellin from symbiotic E. coli strains as a potential therapeutic to restore intestinal immune homeostasis, e.g., for the treatment of IBD patients. |
url |
https://doi.org/10.1371/journal.pbio.3000334 |
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