The Cardiac Syndecan-2 Interactome
The extracellular matrix (ECM) is important in cardiac remodeling and syndecans have gained increased interest in this process due to their ability to convert changes in the ECM to cell signaling. In particular, syndecan-4 has been shown to be important for cardiac remodeling, whereas the role of it...
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2020-08-01
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doaj-25a48045e4be43a29f2e0f26137f34b62020-11-25T03:52:52ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-08-01810.3389/fcell.2020.00792560275The Cardiac Syndecan-2 InteractomeSabrina Bech Mathiesen0Marianne Lunde1Maria Stensland2Marita Martinsen3Tuula A. Nyman4Geir Christensen5Geir Christensen6Cathrine Rein Carlson7Institute for Experimental Medical Research and Oslo University Hospital, University of Oslo, Oslo, NorwayInstitute for Experimental Medical Research and Oslo University Hospital, University of Oslo, Oslo, NorwayDepartment of Immunology, Institute of Clinical Medicine, University of Oslo, Oslo, NorwayInstitute for Experimental Medical Research and Oslo University Hospital, University of Oslo, Oslo, NorwayDepartment of Immunology, Institute of Clinical Medicine, University of Oslo, Oslo, NorwayInstitute for Experimental Medical Research and Oslo University Hospital, University of Oslo, Oslo, NorwayK.G. Jebsen Center for Cardiac Research, University of Oslo, Oslo, NorwayInstitute for Experimental Medical Research and Oslo University Hospital, University of Oslo, Oslo, NorwayThe extracellular matrix (ECM) is important in cardiac remodeling and syndecans have gained increased interest in this process due to their ability to convert changes in the ECM to cell signaling. In particular, syndecan-4 has been shown to be important for cardiac remodeling, whereas the role of its close relative syndecan-2 is largely unknown in the heart. To get more insight into the role of syndecan-2, we here sought to identify interaction partners of syndecan-2 in rat left ventricle. By using three different affinity purification methods combined with mass spectrometry (MS) analysis, we identified 30 novel partners and 9 partners previously described in the literature, which together make up the first cardiac syndecan-2 interactome. Eleven of the novel partners were also verified in HEK293 cells (i.e., AP2A2, CAVIN2, DDX19A, EIF4E, JPH2, MYL12A, NSF, PFDN2, PSMC5, PSMD11, and RRAD). The cardiac syndecan-2 interactome partners formed connections to each other and grouped into clusters mainly involved in cytoskeletal remodeling and protein metabolism, but also into a cluster consisting of a family of novel syndecan-2 interaction partners, the CAVINs. MS analyses revealed that although syndecan-2 was significantly enriched in fibroblast fractions, most of its partners were present in both cardiomyocytes and fibroblasts. Finally, a comparison of the cardiac syndecan-2 and -4 interactomes revealed surprisingly few protein partners in common.https://www.frontiersin.org/article/10.3389/fcell.2020.00792/fullsyndecan-2syndecanproteoglycansinteractomecardiacheart |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sabrina Bech Mathiesen Marianne Lunde Maria Stensland Marita Martinsen Tuula A. Nyman Geir Christensen Geir Christensen Cathrine Rein Carlson |
spellingShingle |
Sabrina Bech Mathiesen Marianne Lunde Maria Stensland Marita Martinsen Tuula A. Nyman Geir Christensen Geir Christensen Cathrine Rein Carlson The Cardiac Syndecan-2 Interactome Frontiers in Cell and Developmental Biology syndecan-2 syndecan proteoglycans interactome cardiac heart |
author_facet |
Sabrina Bech Mathiesen Marianne Lunde Maria Stensland Marita Martinsen Tuula A. Nyman Geir Christensen Geir Christensen Cathrine Rein Carlson |
author_sort |
Sabrina Bech Mathiesen |
title |
The Cardiac Syndecan-2 Interactome |
title_short |
The Cardiac Syndecan-2 Interactome |
title_full |
The Cardiac Syndecan-2 Interactome |
title_fullStr |
The Cardiac Syndecan-2 Interactome |
title_full_unstemmed |
The Cardiac Syndecan-2 Interactome |
title_sort |
cardiac syndecan-2 interactome |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2020-08-01 |
description |
The extracellular matrix (ECM) is important in cardiac remodeling and syndecans have gained increased interest in this process due to their ability to convert changes in the ECM to cell signaling. In particular, syndecan-4 has been shown to be important for cardiac remodeling, whereas the role of its close relative syndecan-2 is largely unknown in the heart. To get more insight into the role of syndecan-2, we here sought to identify interaction partners of syndecan-2 in rat left ventricle. By using three different affinity purification methods combined with mass spectrometry (MS) analysis, we identified 30 novel partners and 9 partners previously described in the literature, which together make up the first cardiac syndecan-2 interactome. Eleven of the novel partners were also verified in HEK293 cells (i.e., AP2A2, CAVIN2, DDX19A, EIF4E, JPH2, MYL12A, NSF, PFDN2, PSMC5, PSMD11, and RRAD). The cardiac syndecan-2 interactome partners formed connections to each other and grouped into clusters mainly involved in cytoskeletal remodeling and protein metabolism, but also into a cluster consisting of a family of novel syndecan-2 interaction partners, the CAVINs. MS analyses revealed that although syndecan-2 was significantly enriched in fibroblast fractions, most of its partners were present in both cardiomyocytes and fibroblasts. Finally, a comparison of the cardiac syndecan-2 and -4 interactomes revealed surprisingly few protein partners in common. |
topic |
syndecan-2 syndecan proteoglycans interactome cardiac heart |
url |
https://www.frontiersin.org/article/10.3389/fcell.2020.00792/full |
work_keys_str_mv |
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