The effects of wild-type and mutant SOD1 on smooth muscle contraction

• Main Authors: Nikolić-Kokić Aleksandra, Oreščanin-Dušić Zorana, Spasojević Ivan, Blagojević Duško, Stević Zorica, Anđus Pavle, Spasić Mihajlo
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2015-01-01
• Series: Archives of Biological Sciences
• Subjects: FALS; mutation; G93A SOD1; WT SOD1; muscle relaxation
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400023N.pdf
• ISSN: 0354-4664; 1821-4339

In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS. [Projekat Ministarstva nauke Republike Srbije, br. 173014 i br. 175083]