RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo Applications

RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo Applications

DNAzymes are catalytically active DNA molecules that are normally isolated through in vitro selection methods, among which RNA-cleaving DNAzymes that catalyze the cleavage of a single RNA linkage embedded within a DNA strand are the most studied group of this DNA enzyme family. Recent advances in DN...

Full description

Bibliographic Details
Main Author: JingJing Zhang
Format: Article
Language:English
Published: MDPI AG 2018-11-01
Series:Catalysts
Subjects:
RNA-cleaving DNAzymes
structural characterization
intracellular imaging
in vivo
cancer therapy
gene regulation
Online Access:https://www.mdpi.com/2073-4344/8/11/550
id doaj-25b0d295712748798601bd6733bf2cf2
record_format Article
spelling doaj-25b0d295712748798601bd6733bf2cf22020-11-25T00:39:33ZengMDPI AGCatalysts2073-43442018-11-0181155010.3390/catal8110550catal8110550RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo ApplicationsJingJing Zhang0Department of Chemistry, University of Illinois at Urbana–Champaign, 601 S. Mathews Ave., Urbana, IL 61801, USADNAzymes are catalytically active DNA molecules that are normally isolated through in vitro selection methods, among which RNA-cleaving DNAzymes that catalyze the cleavage of a single RNA linkage embedded within a DNA strand are the most studied group of this DNA enzyme family. Recent advances in DNA nanotechnology and engineering have generated many RNA-cleaving DNAzymes with unique recognition and catalytic properties. Over the past decade, numerous RNA-cleaving, DNAzymes-based functional probes have been introduced into many research areas, such as in vitro diagnostics, intracellular imaging, and in vivo therapeutics. This review focus on the fundamental insight into RNA-Cleaving DNAzymes and technical tricks for their intracellular and in vivo applications, highlighting the recent progress in the clinical trial of RNA-Cleaving DNAzymes with selected examples. The challenges and opportunities for the future translation of RNA-cleaving DNAzymes for biomedicine are also discussed.https://www.mdpi.com/2073-4344/8/11/550RNA-cleaving DNAzymesstructural characterizationintracellular imagingin vivocancer therapygene regulation
collection DOAJ
language English
format Article
sources DOAJ
author JingJing Zhang
spellingShingle JingJing Zhang
RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo Applications
Catalysts
RNA-cleaving DNAzymes
structural characterization
intracellular imaging
in vivo
cancer therapy
gene regulation
author_facet JingJing Zhang
author_sort JingJing Zhang
title RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo Applications
title_short RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo Applications
title_full RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo Applications
title_fullStr RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo Applications
title_full_unstemmed RNA-Cleaving DNAzymes: Old Catalysts with New Tricks for Intracellular and In Vivo Applications
title_sort rna-cleaving dnazymes: old catalysts with new tricks for intracellular and in vivo applications
publisher MDPI AG
series Catalysts
issn 2073-4344
publishDate 2018-11-01
description DNAzymes are catalytically active DNA molecules that are normally isolated through in vitro selection methods, among which RNA-cleaving DNAzymes that catalyze the cleavage of a single RNA linkage embedded within a DNA strand are the most studied group of this DNA enzyme family. Recent advances in DNA nanotechnology and engineering have generated many RNA-cleaving DNAzymes with unique recognition and catalytic properties. Over the past decade, numerous RNA-cleaving, DNAzymes-based functional probes have been introduced into many research areas, such as in vitro diagnostics, intracellular imaging, and in vivo therapeutics. This review focus on the fundamental insight into RNA-Cleaving DNAzymes and technical tricks for their intracellular and in vivo applications, highlighting the recent progress in the clinical trial of RNA-Cleaving DNAzymes with selected examples. The challenges and opportunities for the future translation of RNA-cleaving DNAzymes for biomedicine are also discussed.
topic RNA-cleaving DNAzymes
structural characterization
intracellular imaging
in vivo
cancer therapy
gene regulation
url https://www.mdpi.com/2073-4344/8/11/550
work_keys_str_mv AT jingjingzhang rnacleavingdnazymesoldcatalystswithnewtricksforintracellularandinvivoapplications
_version_ 1725293800123793408

Similar Items