Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets
The purpose of this study was to fabricate pulsatile tubular cardiac tissue using cell sheet based-tissue engineering. First, we fabricated human induced pluripotent stem cell (hiPSc)-derived cardiomyocyte sheets and normal human dermal fibroblast (NHDF) sheets which are harvested from temperature r...
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doaj-25c47678ba6c4b80a8f0db0933671d072020-11-25T00:12:31ZengElsevierRegenerative Therapy2352-32042019-12-0111297305Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheetsShinpei Tsuruyama0Katsuhisa Matsuura1Katsuhisa Sakaguchi2Tatsuya Shimizu3Cell Processing and Culture Department, Regenerative Medicine Manufacturing Systems Division, SHIBUYA CORPORATION, 2-1 Hokuyodai, Kanazawa-shi, Ishikawa, 920-0177, Japan; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, JapanFaculty of Science and Engineering, Waseda University (TWIns), 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo, 162-8666, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan; Corresponding author. Fax: +81 3 3359 6046.The purpose of this study was to fabricate pulsatile tubular cardiac tissue using cell sheet based-tissue engineering. First, we fabricated human induced pluripotent stem cell (hiPSc)-derived cardiomyocyte sheets and normal human dermal fibroblast (NHDF) sheets which are harvested from temperature responsive culture dishes only by lowering the temperature. Then tubular cardiac tissues are formed by wrapping one hiPSc-derived cardiomyocyte sheet and three NHDF sheets around an octagonal column, and both ends of the tubular tissue were covered with fibrin and collagen gel. The octagonal column with the tubular tissue was connected to an in vitro circulation system in a culture box. After four-day culture, the cardiac tissue survived and pulsated spontaneously in the circulation system. Furthermore, the analysis with a Millar catheter inserted into the cardiac tubes revealed significant inner pressure changes generated by their beating. In addition, the tubular cardiac tissue pulsated in response to the electrical stimulation. Although histological analyses demonstrated that cardiac troponin T-positive cells stratified the inner surface of the tubular tissues, gene expression analyses showed an immature state of these cardiomyocytes. Thus, cell sheet-based tissue engineering realized human pulsatile tubular cardiac tissue fabrication and we believe that these tubular cardiac tissues should contribute to future drug screening and regenerative therapy for heart diseases. Keywords: Human induced pluripotent stem cell, Cardiomyocyte, Three-dimensional tissue model, Tissue engineering, Regenerative medicine, Cell sheethttp://www.sciencedirect.com/science/article/pii/S2352320419300690 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shinpei Tsuruyama Katsuhisa Matsuura Katsuhisa Sakaguchi Tatsuya Shimizu |
spellingShingle |
Shinpei Tsuruyama Katsuhisa Matsuura Katsuhisa Sakaguchi Tatsuya Shimizu Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets Regenerative Therapy |
author_facet |
Shinpei Tsuruyama Katsuhisa Matsuura Katsuhisa Sakaguchi Tatsuya Shimizu |
author_sort |
Shinpei Tsuruyama |
title |
Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets |
title_short |
Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets |
title_full |
Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets |
title_fullStr |
Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets |
title_full_unstemmed |
Pulsatile tubular cardiac tissues fabricated by wrapping human iPS cells-derived cardiomyocyte sheets |
title_sort |
pulsatile tubular cardiac tissues fabricated by wrapping human ips cells-derived cardiomyocyte sheets |
publisher |
Elsevier |
series |
Regenerative Therapy |
issn |
2352-3204 |
publishDate |
2019-12-01 |
description |
The purpose of this study was to fabricate pulsatile tubular cardiac tissue using cell sheet based-tissue engineering. First, we fabricated human induced pluripotent stem cell (hiPSc)-derived cardiomyocyte sheets and normal human dermal fibroblast (NHDF) sheets which are harvested from temperature responsive culture dishes only by lowering the temperature. Then tubular cardiac tissues are formed by wrapping one hiPSc-derived cardiomyocyte sheet and three NHDF sheets around an octagonal column, and both ends of the tubular tissue were covered with fibrin and collagen gel. The octagonal column with the tubular tissue was connected to an in vitro circulation system in a culture box. After four-day culture, the cardiac tissue survived and pulsated spontaneously in the circulation system. Furthermore, the analysis with a Millar catheter inserted into the cardiac tubes revealed significant inner pressure changes generated by their beating. In addition, the tubular cardiac tissue pulsated in response to the electrical stimulation. Although histological analyses demonstrated that cardiac troponin T-positive cells stratified the inner surface of the tubular tissues, gene expression analyses showed an immature state of these cardiomyocytes. Thus, cell sheet-based tissue engineering realized human pulsatile tubular cardiac tissue fabrication and we believe that these tubular cardiac tissues should contribute to future drug screening and regenerative therapy for heart diseases. Keywords: Human induced pluripotent stem cell, Cardiomyocyte, Three-dimensional tissue model, Tissue engineering, Regenerative medicine, Cell sheet |
url |
http://www.sciencedirect.com/science/article/pii/S2352320419300690 |
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