Comparative study of adsorptive role of carbonaceous materials in removal of UV-active impurities of paclitaxel extracts

• Main Authors: Jaber Nasiri, Elaheh Motamedi, Mohammad Reza Naghavi
• Format: Article
• Language: English
• Published: Elsevier 2015-12-01
• Series: Journal of Pharmaceutical Analysis
• Subjects: Paclitaxel; Reduced graphene oxide; Graphite oxide; Purification; Carbon nanotube
• Online Access: http://www.sciencedirect.com/science/article/pii/S2095177915000404

Graphite oxide (GO) and reduced graphene oxide (rGO) nanosheets were synthesized with a low-cost manufacturing method. The morphology and structures of the synthesized samples were studied using X-ray diffraction (XRD), atomic force microscopy (AFM), Fourier-transform infrared (FTIR) and Raman spectroscopy. The efficiencies of GO and rGO as novel candidate adsorbents in the pre-purification of paclitaxel were compared and contrasted with those of commercial graphite (Gt), graphene (G) and multi-wall carbon nanotube (MWCNT). According to UV–vis and HPLC analyses, rGO was evaluated as the best absorbent for the removal of impurities in pre-purification of paclitaxel from plant cell cultures. In contrast, the GO had the poorest proficiency for paclitaxel pre-purification in comparison with the other carbonaceous adsorbents. This is attributed to the existence of many localized defects in the π-structure of GO that is related to weakness of π–π stacking interactions between crude extract impurities and GO.