Genome Analysis of <i>Streptomyces nojiriensis</i> JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus <i>Streptomyces</i>
<i>Streptomyces</i> spp. have been major contributors of novel natural products that are used in many application areas. We found that the nojirimycin (NJ) producer JCM 3382 has antimicrobial activity against <i>Staphylococcus aureus</i> via cellular degradation. Genome analy...
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doaj-25d7e4a1aeb04be6a31f66fdd1c4798b2021-09-26T00:43:12ZengMDPI AGMicroorganisms2076-26072021-08-0191802180210.3390/microorganisms9091802Genome Analysis of <i>Streptomyces nojiriensis</i> JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus <i>Streptomyces</i>Jin-Soo Park0Da-Eun Kim1Sung-Chul Hong2Seung-Young Kim3Hak Cheol Kwon4Chang-Gu Hyun5Jaeyoung Choi6Natural Product Informatics Research Center, Korea Institute of Science and Technology, Gangneung 25451, KoreaNatural Product Informatics Research Center, Korea Institute of Science and Technology, Gangneung 25451, KoreaNatural Product Informatics Research Center, Korea Institute of Science and Technology, Gangneung 25451, KoreaDepartment of Pharmaceutical Engineering & Biotechnology, Sunmoon University, Chungnam 31460, KoreaNatural Product Informatics Research Center, Korea Institute of Science and Technology, Gangneung 25451, KoreaDepartment of Chemistry and Cosmetics, Jeju National University, Jeju 63243, KoreaSmart Farm Research Center, Korea Institute of Science and Technology, Gangneung 25451, Korea<i>Streptomyces</i> spp. have been major contributors of novel natural products that are used in many application areas. We found that the nojirimycin (NJ) producer JCM 3382 has antimicrobial activity against <i>Staphylococcus aureus</i> via cellular degradation. Genome analysis revealed 30 biosynthetic gene clusters, including those responsible for producing antibiotics, including an azasugar NJ. In-depth MS/MS analysis confirmed the production of 1-deoxynojirimycin (DNJ) along with NJ. In addition, the production of tambromycins, setomimycin, and linearmycins was verified by spectroscopic analyses, including LC-MS and NMR. The distribution of the clusters of genes coding for antibiotics in 2061 <i>Streptomyces</i> genomes suggested potential producers of tambromycin, setomimycin, and linearmycin. For a DNJ gene cluster, homologs of <i>gabT1</i> and <i>gutB1</i> were commonly found; however, <i>yktC1</i> was identified in only 112 genomes. The presence of several types of clusters suggests that different strains may produce different types of azasugars. Chemical-profile-inspired comparative genome analysis may facilitate a more accurate assessment of the biosynthetic potential to produce secondary metabolites.https://www.mdpi.com/2076-2607/9/9/1802<i>Streptomyces nojiriensis</i> JCM 3382antibioticsnojirimycinbiosynthetic gene clustersecondary metabolismcomparative genomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin-Soo Park Da-Eun Kim Sung-Chul Hong Seung-Young Kim Hak Cheol Kwon Chang-Gu Hyun Jaeyoung Choi |
spellingShingle |
Jin-Soo Park Da-Eun Kim Sung-Chul Hong Seung-Young Kim Hak Cheol Kwon Chang-Gu Hyun Jaeyoung Choi Genome Analysis of <i>Streptomyces nojiriensis</i> JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus <i>Streptomyces</i> Microorganisms <i>Streptomyces nojiriensis</i> JCM 3382 antibiotics nojirimycin biosynthetic gene cluster secondary metabolism comparative genomics |
author_facet |
Jin-Soo Park Da-Eun Kim Sung-Chul Hong Seung-Young Kim Hak Cheol Kwon Chang-Gu Hyun Jaeyoung Choi |
author_sort |
Jin-Soo Park |
title |
Genome Analysis of <i>Streptomyces nojiriensis</i> JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus <i>Streptomyces</i> |
title_short |
Genome Analysis of <i>Streptomyces nojiriensis</i> JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus <i>Streptomyces</i> |
title_full |
Genome Analysis of <i>Streptomyces nojiriensis</i> JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus <i>Streptomyces</i> |
title_fullStr |
Genome Analysis of <i>Streptomyces nojiriensis</i> JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus <i>Streptomyces</i> |
title_full_unstemmed |
Genome Analysis of <i>Streptomyces nojiriensis</i> JCM 3382 and Distribution of Gene Clusters for Three Antibiotics and an Azasugar across the Genus <i>Streptomyces</i> |
title_sort |
genome analysis of <i>streptomyces nojiriensis</i> jcm 3382 and distribution of gene clusters for three antibiotics and an azasugar across the genus <i>streptomyces</i> |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-08-01 |
description |
<i>Streptomyces</i> spp. have been major contributors of novel natural products that are used in many application areas. We found that the nojirimycin (NJ) producer JCM 3382 has antimicrobial activity against <i>Staphylococcus aureus</i> via cellular degradation. Genome analysis revealed 30 biosynthetic gene clusters, including those responsible for producing antibiotics, including an azasugar NJ. In-depth MS/MS analysis confirmed the production of 1-deoxynojirimycin (DNJ) along with NJ. In addition, the production of tambromycins, setomimycin, and linearmycins was verified by spectroscopic analyses, including LC-MS and NMR. The distribution of the clusters of genes coding for antibiotics in 2061 <i>Streptomyces</i> genomes suggested potential producers of tambromycin, setomimycin, and linearmycin. For a DNJ gene cluster, homologs of <i>gabT1</i> and <i>gutB1</i> were commonly found; however, <i>yktC1</i> was identified in only 112 genomes. The presence of several types of clusters suggests that different strains may produce different types of azasugars. Chemical-profile-inspired comparative genome analysis may facilitate a more accurate assessment of the biosynthetic potential to produce secondary metabolites. |
topic |
<i>Streptomyces nojiriensis</i> JCM 3382 antibiotics nojirimycin biosynthetic gene cluster secondary metabolism comparative genomics |
url |
https://www.mdpi.com/2076-2607/9/9/1802 |
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