Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy

Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy

1H-NMR spectroscopy was applied for the quantitative analysis of cnicin, a bioactive germacranolide type sesquiterpene lactone, in the aerial parts of seven wild-growing Centaurea species collected in Serbia and Montenegro. The analysis was performed by comparison of the integral of the one-proton s...

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Main Authors: IRIS DJORDJEVIC, PEDJA JANACKOVIC, VLATKA VAJS, SLOBODAN MILOSAVLJEVIC, VELE TESEVIC, MILKA JADRANIN, IVAN VUCKOVIC
Format: Article
Language:English
Published: Serbian Chemical Society 2007-12-01
Series:Journal of the Serbian Chemical Society
Subjects:
1H-NMR
Centaurea
cnicin
BHT
Online Access:http://www.shd.org.yu/JSCS/Vol72/No12/JSCS_V72_No12-12.pdf
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spelling doaj-25d7f6299a38489d8daa196762475ce62020-11-24T21:53:59ZengSerbian Chemical Society Journal of the Serbian Chemical Society0352-51392007-12-01721212751280Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy IRIS DJORDJEVICPEDJA JANACKOVICVLATKA VAJSSLOBODAN MILOSAVLJEVICVELE TESEVICMILKA JADRANINIVAN VUCKOVIC1H-NMR spectroscopy was applied for the quantitative analysis of cnicin, a bioactive germacranolide type sesquiterpene lactone, in the aerial parts of seven wild-growing Centaurea species collected in Serbia and Montenegro. The analysis was performed by comparison of the integral of the one-proton signal of cnicin (H-13, δ 5.75) with that of the two-proton singlet (δ 6.98) of 2,6-bis(1,1-dimethylethyl)-4-methylphenol (BHT), used as the internal standard. Cnicin, within concentration the range 1.06–6.12 mg/g, calculated per weight of the fresh plant material was detected in six species, the exception being C. salonitana. This method allows the rapid and simple quantification of cnicin without any pre-purification step.http://www.shd.org.yu/JSCS/Vol72/No12/JSCS_V72_No12-12.pdf1H-NMRCentaureacnicinBHT
collection DOAJ
language English
format Article
sources DOAJ
author IRIS DJORDJEVIC
PEDJA JANACKOVIC
VLATKA VAJS
SLOBODAN MILOSAVLJEVIC
VELE TESEVIC
MILKA JADRANIN
IVAN VUCKOVIC
spellingShingle IRIS DJORDJEVIC
PEDJA JANACKOVIC
VLATKA VAJS
SLOBODAN MILOSAVLJEVIC
VELE TESEVIC
MILKA JADRANIN
IVAN VUCKOVIC
Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy
Journal of the Serbian Chemical Society
1H-NMR
Centaurea
cnicin
BHT
author_facet IRIS DJORDJEVIC
PEDJA JANACKOVIC
VLATKA VAJS
SLOBODAN MILOSAVLJEVIC
VELE TESEVIC
MILKA JADRANIN
IVAN VUCKOVIC
author_sort IRIS DJORDJEVIC
title Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy
title_short Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy
title_full Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy
title_fullStr Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy
title_full_unstemmed Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy
title_sort quantitative analysis of sesquiterpene lactone cnicin in seven centaurea species wild-growing in serbia and montenegro using 1h-nmr spectroscopy
publisher Serbian Chemical Society
series Journal of the Serbian Chemical Society
issn 0352-5139
publishDate 2007-12-01
description 1H-NMR spectroscopy was applied for the quantitative analysis of cnicin, a bioactive germacranolide type sesquiterpene lactone, in the aerial parts of seven wild-growing Centaurea species collected in Serbia and Montenegro. The analysis was performed by comparison of the integral of the one-proton signal of cnicin (H-13, δ 5.75) with that of the two-proton singlet (δ 6.98) of 2,6-bis(1,1-dimethylethyl)-4-methylphenol (BHT), used as the internal standard. Cnicin, within concentration the range 1.06–6.12 mg/g, calculated per weight of the fresh plant material was detected in six species, the exception being C. salonitana. This method allows the rapid and simple quantification of cnicin without any pre-purification step.
topic 1H-NMR
Centaurea
cnicin
BHT
url http://www.shd.org.yu/JSCS/Vol72/No12/JSCS_V72_No12-12.pdf
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AT pedjajanackovic quantitativeanalysisofsesquiterpenelactonecnicininsevencentaureaspecieswildgrowinginserbiaandmontenegrousing1hnmrspectroscopy
AT vlatkavajs quantitativeanalysisofsesquiterpenelactonecnicininsevencentaureaspecieswildgrowinginserbiaandmontenegrousing1hnmrspectroscopy
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