Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study
Resveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-02-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/4/1453 |
id |
doaj-25d813c5e71c488e9504d994384a0c39 |
---|---|
record_format |
Article |
spelling |
doaj-25d813c5e71c488e9504d994384a0c392020-11-25T02:37:07ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01214145310.3390/ijms21041453ijms21041453Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled StudyLynn M. Martin0Philip J. Johnson1Juliana R. Amorim2Amy E. DeClue3Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USADepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USADepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USADepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USAResveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function have not been reported in horses. The objective of this study was to determine the effects of a commercially available, orally administered resveratrol product on innate immune functions in healthy adult horses. Whole blood was collected from 12 horses prior to and following 3 weeks of treatment with either the manufacturer’s recommended dose of resveratrol or placebo. Phagocytosis, oxidative burst and pathogen associated molecular pattern (PAMP) motif-stimulated leukocyte production of TNF and IL-1β were compared pre- and post-treatment between treatment groups. Phagocytosis and oxidative burst capacity were evaluated via flow cytometry. Tumor necrosis factor and IL-1β were measured using cytotoxicity and ELISA assays, respectively. There were no significant differences in phagocytosis, oxidative burst or stimulated TNF or IL-1β production between resveratrol and placebo treatment groups. Orally administered resveratrol at a routinely recommended dose for a duration of 3 weeks did not significantly affect phagocytic activity, oxidative burst function or PAMP-stimulated leukocyte cytokine production.https://www.mdpi.com/1422-0067/21/4/1453cytokineendotoxinequineinflammationlipopolysaccharidenutraceuticalresveratrolsepsissupplement |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lynn M. Martin Philip J. Johnson Juliana R. Amorim Amy E. DeClue |
spellingShingle |
Lynn M. Martin Philip J. Johnson Juliana R. Amorim Amy E. DeClue Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study International Journal of Molecular Sciences cytokine endotoxin equine inflammation lipopolysaccharide nutraceutical resveratrol sepsis supplement |
author_facet |
Lynn M. Martin Philip J. Johnson Juliana R. Amorim Amy E. DeClue |
author_sort |
Lynn M. Martin |
title |
Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study |
title_short |
Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study |
title_full |
Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study |
title_fullStr |
Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study |
title_full_unstemmed |
Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study |
title_sort |
effects of orally administered resveratrol on tnf, il-1β, leukocyte phagocytic activity and oxidative burst function in horses: a prospective, randomized, double-blinded, placebo-controlled study |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-02-01 |
description |
Resveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function have not been reported in horses. The objective of this study was to determine the effects of a commercially available, orally administered resveratrol product on innate immune functions in healthy adult horses. Whole blood was collected from 12 horses prior to and following 3 weeks of treatment with either the manufacturer’s recommended dose of resveratrol or placebo. Phagocytosis, oxidative burst and pathogen associated molecular pattern (PAMP) motif-stimulated leukocyte production of TNF and IL-1β were compared pre- and post-treatment between treatment groups. Phagocytosis and oxidative burst capacity were evaluated via flow cytometry. Tumor necrosis factor and IL-1β were measured using cytotoxicity and ELISA assays, respectively. There were no significant differences in phagocytosis, oxidative burst or stimulated TNF or IL-1β production between resveratrol and placebo treatment groups. Orally administered resveratrol at a routinely recommended dose for a duration of 3 weeks did not significantly affect phagocytic activity, oxidative burst function or PAMP-stimulated leukocyte cytokine production. |
topic |
cytokine endotoxin equine inflammation lipopolysaccharide nutraceutical resveratrol sepsis supplement |
url |
https://www.mdpi.com/1422-0067/21/4/1453 |
work_keys_str_mv |
AT lynnmmartin effectsoforallyadministeredresveratrolontnfil1bleukocytephagocyticactivityandoxidativeburstfunctioninhorsesaprospectiverandomizeddoubleblindedplacebocontrolledstudy AT philipjjohnson effectsoforallyadministeredresveratrolontnfil1bleukocytephagocyticactivityandoxidativeburstfunctioninhorsesaprospectiverandomizeddoubleblindedplacebocontrolledstudy AT julianaramorim effectsoforallyadministeredresveratrolontnfil1bleukocytephagocyticactivityandoxidativeburstfunctioninhorsesaprospectiverandomizeddoubleblindedplacebocontrolledstudy AT amyedeclue effectsoforallyadministeredresveratrolontnfil1bleukocytephagocyticactivityandoxidativeburstfunctioninhorsesaprospectiverandomizeddoubleblindedplacebocontrolledstudy |
_version_ |
1724796599294492672 |