Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts

• Main Authors: L. Zhang, Y. Ding, G.Z. Rao, D. Miao
• Format: Article
• Language: English
• Published: Associação Brasileira de Divulgação Científica 2016-01-01
• Series: Brazilian Journal of Medical and Biological Research
• Subjects: Human periodontal ligament fibroblasts; Interleukin-10; Glucose; Osteoprotegerin; Receptor activator of nuclear factor-κB ligand
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605&lng=en&tlng=en

The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.