Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses.
As a partner of the European Virus Archive (EVA) FP7 project, our laboratory maintains a large collection of freeze-dried viruses. The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilizati...
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doaj-25eea774cdd9419fbcf3e30b714e79ab2020-11-24T21:48:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e011896310.1371/journal.pone.0118963Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses.Boris PastorinoCecile BarontiErnest A GouldRemi N CharrelXavier de LamballerieAs a partner of the European Virus Archive (EVA) FP7 project, our laboratory maintains a large collection of freeze-dried viruses. The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. However, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. In order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20°C, +4°C and +20°C) for one week, the two latter mimicking suboptimal shipping conditions. The Tissue Culture Infectious Dose 50% (TCID50) assay was used to compare the titres of infectious virus. The results obtained using four relevant and model viruses (enveloped/non enveloped RNA/DNA viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection.http://europepmc.org/articles/PMC4414529?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Boris Pastorino Cecile Baronti Ernest A Gould Remi N Charrel Xavier de Lamballerie |
spellingShingle |
Boris Pastorino Cecile Baronti Ernest A Gould Remi N Charrel Xavier de Lamballerie Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses. PLoS ONE |
author_facet |
Boris Pastorino Cecile Baronti Ernest A Gould Remi N Charrel Xavier de Lamballerie |
author_sort |
Boris Pastorino |
title |
Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses. |
title_short |
Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses. |
title_full |
Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses. |
title_fullStr |
Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses. |
title_full_unstemmed |
Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses. |
title_sort |
effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
As a partner of the European Virus Archive (EVA) FP7 project, our laboratory maintains a large collection of freeze-dried viruses. The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. However, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. In order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20°C, +4°C and +20°C) for one week, the two latter mimicking suboptimal shipping conditions. The Tissue Culture Infectious Dose 50% (TCID50) assay was used to compare the titres of infectious virus. The results obtained using four relevant and model viruses (enveloped/non enveloped RNA/DNA viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection. |
url |
http://europepmc.org/articles/PMC4414529?pdf=render |
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