Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus
Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vector...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hanrimwon Publishing Company
2016-08-01
|
Series: | The Plant Pathology Journal |
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968648 |
id |
doaj-25eea7bd4b7644499d004b3176bf10a6 |
---|---|
record_format |
Article |
spelling |
doaj-25eea7bd4b7644499d004b3176bf10a62020-11-24T23:07:17ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542016-08-0132437137610.5423/PPJ.NT.11.2015.0237PPJ.NT.11.2015.0237Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent VirusSang-Ho Park0Hoseong Choi1Semin Kim2Won Kyong Cho3Kook-Hyung Kim4Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaGrapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the reporter gene, green fluorescence protein (GFP), but the expression of GFP was not detected due to the necrotic cell death on the infiltrated leaves. The p19 silencing suppressor of GALV was engineered to inactivate its expression and GFP was successfully expressed with unrelated silencing suppressor, HC-Pro, from soybean mosaic virus. The pGMG vector was used to knock down magnesium chelatase (ChlH) gene in N. benthamaina and the silencing phenotype was clearly observed on systemic leaves. Altogether, the GALV-derived vector is expected to be an attractive tool for useful gene expression and VIGS vectors in grapevine as well as N. benthamiana.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968648Grapevine Algerian latent virusvirus-based expression vectorvirus-induced gene silencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sang-Ho Park Hoseong Choi Semin Kim Won Kyong Cho Kook-Hyung Kim |
spellingShingle |
Sang-Ho Park Hoseong Choi Semin Kim Won Kyong Cho Kook-Hyung Kim Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus The Plant Pathology Journal Grapevine Algerian latent virus virus-based expression vector virus-induced gene silencing |
author_facet |
Sang-Ho Park Hoseong Choi Semin Kim Won Kyong Cho Kook-Hyung Kim |
author_sort |
Sang-Ho Park |
title |
Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus |
title_short |
Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus |
title_full |
Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus |
title_fullStr |
Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus |
title_full_unstemmed |
Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus |
title_sort |
development of virus-induced gene expression and silencing vector derived from grapevine algerian latent virus |
publisher |
Hanrimwon Publishing Company |
series |
The Plant Pathology Journal |
issn |
1598-2254 |
publishDate |
2016-08-01 |
description |
Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the reporter gene, green fluorescence protein (GFP), but the expression of GFP was not detected due to the necrotic cell death on the infiltrated leaves. The p19 silencing suppressor of GALV was engineered to inactivate its expression and GFP was successfully expressed with unrelated silencing suppressor, HC-Pro, from soybean mosaic virus. The pGMG vector was used to knock down magnesium chelatase (ChlH) gene in N. benthamaina and the silencing phenotype was clearly observed on systemic leaves. Altogether, the GALV-derived vector is expected to be an attractive tool for useful gene expression and VIGS vectors in grapevine as well as N. benthamiana. |
topic |
Grapevine Algerian latent virus virus-based expression vector virus-induced gene silencing |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968648 |
work_keys_str_mv |
AT sanghopark developmentofvirusinducedgeneexpressionandsilencingvectorderivedfromgrapevinealgerianlatentvirus AT hoseongchoi developmentofvirusinducedgeneexpressionandsilencingvectorderivedfromgrapevinealgerianlatentvirus AT seminkim developmentofvirusinducedgeneexpressionandsilencingvectorderivedfromgrapevinealgerianlatentvirus AT wonkyongcho developmentofvirusinducedgeneexpressionandsilencingvectorderivedfromgrapevinealgerianlatentvirus AT kookhyungkim developmentofvirusinducedgeneexpressionandsilencingvectorderivedfromgrapevinealgerianlatentvirus |
_version_ |
1725619086496366592 |